Abstract
WRKYs are one of the largest transcription factor (TF) families and play an important role in plant resistance to various stresses. TaWRKY133, a group I WRKY protein, responds to a variety of abiotic stresses, including PEG treatment. The TaWRKY133 protein is located in the nucleus of tobacco epidermal cells, and both its N-terminal and C-terminal domains exhibit transcriptional activation activity. Overexpression of TaWRKY133 reduced drought tolerance in Arabidopsis thaliana, as reflected by a lower germination rate, shorter roots, higher stomatal aperture, poorer growth and lower antioxidant enzyme activities under drought treatment. Moreover, expression levels of stress-related genes (DREB2A, RD29A, RD29B, ABF1, ABA2, ABI1, SOD (Cu/Zn), POD1 and CAT1) were downregulated in transgenic Arabidopsis under drought stress. Gene silencing of TaWRKY133 enhanced the drought tolerance of wheat, as reflected in better growth, higher antioxidant enzyme activities, and higher expression levels of stress-related genes including DREB1, DREB3, ABF, ERF3, SOD (Fe), POD, CAT and P5CS. In conclusion, these results suggest that TaWRKY133 might reduce drought tolerance in plants by regulating the expression of stress-related genes.
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Claims 8
Claim · model-written summary
TaWRKY133 responded to abiotic stresses including PEG.
- Taxon
- PlantsViridiplantaet0
- Treatment
- PEG treatmente0
- Gene
- TaWRKY133e0
- Outcome
- responds to a variety of abiotic stressese0
Verbatim
e0“TaWRKY133, a group I WRKY protein, responds to a variety of abiotic stresses, including PEG treatment”
t0“Transgenic Plants”
Claim · model-written summary
TaWRKY133 was localized to nuclei of tobacco epidermal cells.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- epidermal cellse0
- Gene
- TaWRKY133e0
- Outcome
- located in the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The TaWRKY133 protein is located in the nucleus of tobacco epidermal cells”
Claim · model-written summary
Both TaWRKY133 terminal domains showed transcriptional activation.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- TaWRKY133e2
- Outcome
- transcriptional activation activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both its N-terminal and C-terminal domains exhibit transcriptional activation activity”
e1“tobacco epidermal cells”
e2“TaWRKY133 protein”
Claim · model-written summary
TaWRKY133 overexpression reduced Arabidopsis drought tolerance, germination, roots, growth and antioxidant activity while increasing stomatal aperture.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- Overexpression of TaWRKY133e0
- Gene
- TaWRKY133e0
- Outcome
- drought tolerancee0
- Direction
- reducede0
- Qualifier
- lower germination rate, shorter roots, higher stomatal aperture, poorer growth and lower antioxidant enzyme activitiese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of TaWRKY133 reduced drought tolerance in Arabidopsis thaliana, as reflected by a lower germination rate, shorter roots, higher stomatal aperture, poorer growth and lower antioxidant enzyme activities under drought treatment”
Claim · model-written summary
Stress-related gene expression was downregulated in transgenic Arabidopsis under drought.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- expression levels of stress-related genese0
- Direction
- downregulatede0
- Qualifier
- under drought stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression levels of stress-related genes (DREB2A, RD29A, RD29B, ABF1, ABA2, ABI1, SOD (Cu/Zn), POD1 and CAT1) were downregulated in transgenic Arabidopsis under drought stress”
Claim · model-written summary
TaWRKY133 silencing enhanced wheat drought tolerance, growth and antioxidant activity.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- Gene silencing of TaWRKY133e0
- Outcome
- drought tolerancee0
- Direction
- enhancede0
- Qualifier
- better growth, higher antioxidant enzyme activitiese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gene silencing of TaWRKY133 enhanced the drought tolerance of wheat, as reflected in better growth, higher antioxidant enzyme activities”
Claim · model-written summary
TaWRKY133 silencing increased stress-related gene expression in wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Gene silencing of TaWRKY133e0
- Outcome
- expression levels of stress-related genese0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gene silencing of TaWRKY133 enhanced the drought tolerance of wheat, as reflected in better growth, higher antioxidant enzyme activities, and higher expression levels of stress-related genes including DREB1, DREB3, ABF, ERF3, SOD (Fe), POD, CAT and P5CS”
Claim · model-written summary
The authors suggest TaWRKY133 reduces drought tolerance via stress-related gene regulation.
Verbatim
e0“TaWRKY133 might reduce drought tolerance in plants by regulating the expression of stress-related genes”
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