Abstract
Efficient phosphate (Pi) uptake and utilisation are essential for promoting crop yield. However, the underlying molecular mechanism is still poorly understood in complex crop species such as hexaploid wheat. Here we report that TaPHT1;9-4B and its transcriptional regulator TaMYB4-7D function in Pi acquisition, translocation and plant growth in bread wheat. TaPHT1;9-4B, a high-affinity Pi transporter highly upregulated in roots by Pi deficiency, was identified using quantitative proteomics. Disruption of TaPHT1;9-4B function by BSMV-VIGS or CRISPR editing impaired wheat tolerance to Pi deprivation, whereas transgenic expression of TaPHT1;9-4B in rice improved Pi uptake and plant growth. Using yeast-one-hybrid assay, we isolated TaMYB4-7D, a R2R3 MYB transcription factor that could activate TaPHT1;9-4B expression by binding to its promoter. Silencing TaMYB4-7D decreased TaPHT1;9-4B expression, Pi uptake and plant growth. Four promoter haplotypes were identified for TaPHT1;9-4B, with Hap3 showing significant positive associations with TaPHT1;9-4B transcript level, growth performance and phosphorus (P) content in wheat plants. A functional marker was therefore developed for tagging Hap3. Collectively, our data shed new light on the molecular mechanism controlling Pi acquisition and utilisation in bread wheat. TaPHT1;9-4B and TaMYB4-7D may aid further research towards the development of P efficient crop cultivars.
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Claims 7
Claim · model-written summary
TaPHT1;9-4B, a high-affinity phosphate transporter identified by quantitative proteomics, is highly upregulated in roots by phosphate deficiency.
- Taxon
- bread wheat
Triticum aestivumt0 - Treatment
- Pi deficiencye0
- Gene
- TaPHT1;9-4Be0
- Outcome
- highly upregulated in rootse0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in bread wheat”
e0“TaPHT1;9-4B, a high-affinity Pi transporter highly upregulated in roots by Pi deficiency, was identified using quantitative proteomics”
Claim · model-written summary
Disrupting TaPHT1;9-4B function by BSMV-VIGS or CRISPR editing impaired wheat tolerance to phosphate deprivation.
- Taxon
- bread wheatTriticum aestivumt0
- Treatment
- Disruption of TaPHT1;9-4B function by BSMV-VIGS or CRISPR editinge1
- Gene
- TaPHT1;9-4Be1
- Outcome
- tolerance to Pi deprivatione1
- Direction
- impairede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in bread wheat”
e1“Disruption of TaPHT1;9-4B function by BSMV-VIGS or CRISPR editing impaired wheat tolerance to Pi deprivation”
Claim · model-written summary
Transgenic expression of TaPHT1;9-4B in rice improved phosphate uptake and plant growth.
- Taxon
- rice
Oryza sativae2 - Treatment
- transgenic expression of TaPHT1;9-4Be2
- Gene
- TaPHT1;9-4Be2
- Outcome
- Pi uptake and plant growthe2
- Direction
- improvede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“transgenic expression of TaPHT1;9-4B in rice improved Pi uptake and plant growth”
Claim · model-written summary
TaMYB4-7D, an R2R3 MYB transcription factor, activates TaPHT1;9-4B expression by binding its promoter.
- Taxon
- bread wheatTriticum aestivumt0
- Gene
- TaMYB4-7De3
- Outcome
- activate TaPHT1;9-4B expressione3
- Qualifier
- by binding to its promotere3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in bread wheat”
e3“we isolated TaMYB4-7D, a R2R3 MYB transcription factor that could activate TaPHT1;9-4B expression by binding to its promoter”
Claim · model-written summary
Silencing TaMYB4-7D decreased TaPHT1;9-4B expression, phosphate uptake and plant growth.
- Taxon
- bread wheatTriticum aestivumt0
- Treatment
- Silencing TaMYB4-7De4
- Gene
- TaMYB4-7De4
- Outcome
- TaPHT1;9-4B expression, Pi uptake and plant growthe4
- Direction
- decreasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in bread wheat”
e4“Silencing TaMYB4-7D decreased TaPHT1;9-4B expression, Pi uptake and plant growth”
Claim · model-written summary
Promoter haplotype Hap3 of TaPHT1;9-4B shows significant positive associations with transcript level, growth performance and phosphorus content in wheat plants.
- Taxon
- wheatTriticum aestivume5
- Material
- Hap3e5
- Gene
- TaPHT1;9-4Be5
- Outcome
- transcript level, growth performance and phosphorus (P) contente5
- Direction
- positive associationse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Four promoter haplotypes were identified for TaPHT1;9-4B, with Hap3 showing significant positive associations with TaPHT1;9-4B transcript level, growth performance and phosphorus (P) content in wheat plants”
Claim · model-written summary
A functional marker was developed for tagging the Hap3 haplotype.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- Hap3e6
- Outcome
- functional markere6
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in bread wheat”
e6“A functional marker was therefore developed for tagging Hap3”
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