Abstract
Heat stress caused by increasing global temperature has become a major factor limiting yield in wheat. Heat shock transcription factors (Hsfs), as the primary regulators in plant responses to heat stress, play essential roles in modulating both basal and acquired thermotolerance in plants. However, the underlying molecular mechanisms remain to be elucidated. By analysing the wheat transcriptome after subjecting wheat to heat treatments for different time intervals, we identified gene TaHsfA2h that showed a significant positive regulatory response to heat stress. Heat stress tolerance was enhanced by overexpression of TaHsfA2h and constrained by its RNA interference. RNA-seq analysis demonstrated that the overexpression of TaHsfA2h significantly enhanced the expression levels of genes involved in ABA and ROS signalling pathways. Additionally, we identified TaABF5b, a critical regulatory factor in the ABA signalling pathway, as being capable of modulating the expression of TaHsfA2h. Notably, TaHsfA2h interacted with TaHsfC2a both in vivo and in vitro. Similarly, overexpression of TaHsfC2a significantly enhanced heat stress tolerance, whereas knockout dramatically reduced tolerance. The presence of TaHsfC2a significantly enhanced the regulatory activity of TaHsfA2h. TaHsfA2h and TaHsfC2a can co-regulate the expression levels of heat stress tolerance-related genes, including TaNCED2B, TaPOD4 and TaHSP26, thereby enhancing wheat's tolerance to heat stress. Overall, our findings revealed a positive regulatory function of the ABF5b-HsfA2h/HsfC2a-NCED2b/POD4/HSP26 module on wheat heat stress tolerance. This discovery further expanded the functionality of a plant heat stress response model, providing a theoretical foundation for the development of heat-tolerant wheat varieties.
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Claims 11
Claim · model-written summary
TaHsfA2h responded positively to heat stress.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- heat stresse0
- Gene
- TaHsfA2he0
- Outcome
- regulatory response to heat stresse0
- Direction
- positivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gene TaHsfA2h that showed a significant positive regulatory response to heat stress”
t0“wheat”
Claim · model-written summary
TaHsfA2h overexpression enhanced heat tolerance.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- overexpression of TaHsfA2he0
- Gene
- TaHsfA2he0
- Outcome
- Heat stress tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Heat stress tolerance was enhanced by overexpression of TaHsfA2h”
t0“wheat”
Claim · model-written summary
TaHsfA2h RNA interference constrained heat tolerance.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- RNA interferencee0
- Gene
- TaHsfA2he0
- Outcome
- Heat stress tolerancee0
- Direction
- constrainede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Heat stress tolerance was enhanced by overexpression of TaHsfA2h and constrained by its RNA interference”
t0“wheat”
Claim · model-written summary
TaHsfA2h overexpression increased ABA and ROS pathway gene expression.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- overexpression of TaHsfA2he0
- Gene
- TaHsfA2he0
- Outcome
- expression levels of genes involved in ABA and ROS signalling pathwayse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of TaHsfA2h significantly enhanced the expression levels of genes involved in ABA and ROS signalling pathways”
t0“wheat”
Claim · model-written summary
TaABF5b can modulate TaHsfA2h expression.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaABF5be0
- Outcome
- expression of TaHsfA2he0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaABF5b, a critical regulatory factor in the ABA signalling pathway, as being capable of modulating the expression of TaHsfA2h”
t0“wheat”
Claim · model-written summary
TaHsfA2h interacted with TaHsfC2a in vivo and in vitro.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaHsfA2he0
- Outcome
- interacted with TaHsfC2ae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaHsfA2h interacted with TaHsfC2a both in vivo and in vitro”
t0“wheat”
Claim · model-written summary
TaHsfC2a overexpression enhanced heat tolerance.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- overexpression of TaHsfC2ae0
- Gene
- TaHsfC2ae0
- Outcome
- heat stress tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of TaHsfC2a significantly enhanced heat stress tolerance”
t0“wheat”
Claim · model-written summary
TaHsfC2a knockout reduced heat tolerance.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of TaHsfC2a significantly enhanced heat stress tolerance, whereas knockout dramatically reduced tolerance”
t0“wheat”
Claim · model-written summary
TaHsfC2a enhanced TaHsfA2h regulatory activity.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- presence of TaHsfC2ae0
- Gene
- TaHsfC2ae0
- Outcome
- regulatory activity of TaHsfA2he0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of TaHsfC2a significantly enhanced the regulatory activity of TaHsfA2h”
t0“wheat”
Claim · model-written summary
TaHsfA2h and TaHsfC2a co regulate TaNCED2B, TaPOD4 and TaHSP26 expression.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaHsfA2h and TaHsfC2ae0
- Outcome
- expression levels of heat stress tolerance-related genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaHsfA2h and TaHsfC2a can co-regulate the expression levels of heat stress tolerance-related genes, including TaNCED2B, TaPOD4 and TaHSP26”
t0“wheat”
Claim · model-written summary
The regulatory module positively regulates wheat heat tolerance.
- Taxon
- wheatTriticum aestivume0
- Gene
- ABF5b-HsfA2h/HsfC2a-NCED2b/POD4/HSP26 modulee0
- Outcome
- wheat heat stress tolerancee0
- Direction
- positivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“positive regulatory function of the ABF5b-HsfA2h/HsfC2a-NCED2b/POD4/HSP26 module on wheat heat stress tolerance”
The same questions in other papers
loss of function · tolerance: abiotic · Triticum aestivum (wheat)Positive and negative results
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- positive
- positive
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9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.