Paper · 1983 · Journal of the American Society for Horticultural Science

In Vitro Acclimatization of Aseptically Cultured Plantlets to Humidity

K. Wardle; E.B. Dobbs; K.C. Short

8
claims
13
verbatim quotes
105
citations
2
research areas
✓
Open access · cc-by-nc-nd

Abstract

Abstract A method is described whereby relative humidity is reduced in the atmosphere of agar-solidified tissue cultures. Plantlets of chrysanthemum ( Chrysanthemum X morifolium Ramat.) cultured under low humidity exhibited a high mortality rate and those that survived were smaller and had few roots. Severe retardation of stomatal development occurred under reduced humidity and mature stomata had considerably narrower apertures than did plantlets grown at about 100% relative humidity. Plantlets of cauliflower ( Brassica oleracea L. Botrytis group) cultured under reduced humidity also exhibited reduced growth but, in addition, developed large quantities of surface wax and had lower rates of water loss. The results are discussed in relation to the potential uses of the technique for acclimatization of plantlets prior to transfer to soil.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 8

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Chrysanthemum plantlets cultured under low humidity showed a high mortality rate.

Taxon
chrysanthemumChrysanthemume1
Treatment
cultured under low humiditye1
Outcome
mortality ratee1
Direction
increaseinferrede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Chrysanthemum
Chrysanthemum · Taxon · picture from its Wikimedia Commons categoryLucia Condac · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Plantlets of chrysanthemum ( Chrysanthemum X morifolium Ramat.) cultured under low humidity exhibited a high mortality rate”

✓ located verbatim in the source · abstract, characters 122–245 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Chrysanthemum plantlets that survived low-humidity culture were smaller.

Taxon
chrysanthemumChrysanthemume1
Treatment
cultured under low humiditye1
Outcome
those that survivede2
Direction
smallere2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Chrysanthemum
Chrysanthemum · Taxon · picture from its Wikimedia Commons categoryLucia Condac · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Plantlets of chrysanthemum ( Chrysanthemum X morifolium Ramat.) cultured under low humidity exhibited a high mortality rate”

✓ located verbatim in the source · abstract, characters 122–245 · show in text ↑
e2

“those that survived were smaller and had few roots”

✓ located verbatim in the source · abstract, characters 250–300 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Chrysanthemum plantlets that survived low-humidity culture had few roots.

Taxon
chrysanthemumChrysanthemume1
Treatment
cultured under low humiditye1
Outcome
rootse2
Direction
fewe2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Chrysanthemum
Chrysanthemum · Taxon · picture from its Wikimedia Commons categoryLucia Condac · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Plantlets of chrysanthemum ( Chrysanthemum X morifolium Ramat.) cultured under low humidity exhibited a high mortality rate”

✓ located verbatim in the source · abstract, characters 122–245 · show in text ↑
e2

“those that survived were smaller and had few roots”

✓ located verbatim in the source · abstract, characters 250–300 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Reduced humidity severely retarded stomatal development in the tissue-cultured plantlets.

Treatment
reduced humiditye3
Outcome
stomatal developmente3
Direction
retardatione3

Not reportedTaxonComparator

Verbatim

e3

“Severe retardation of stomatal development occurred under reduced humidity”

✓ located verbatim in the source · abstract, characters 302–376 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Mature stomata under reduced humidity had considerably narrower apertures than stomata of plantlets grown at about 100% relative humidity.

Organ / stage
mature stomatae4
Treatment
reduced humiditye3
Outcome
aperturese4
Direction
narrowere4
Comparator
plantlets grown at about 100% relative humiditye4

Not reportedTaxon

Verbatim

e3

“Severe retardation of stomatal development occurred under reduced humidity”

✓ located verbatim in the source · abstract, characters 302–376 · show in text ↑
e4

“mature stomata had considerably narrower apertures than did plantlets grown at about 100% relative humidity”

✓ located verbatim in the source · abstract, characters 381–488 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cauliflower plantlets cultured under reduced humidity showed reduced growth.

Treatment
cultured under reduced humiditye5
Outcome
growthe5
Direction
reducede5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e5

“Plantlets of cauliflower ( Brassica oleracea L. Botrytis group) cultured under reduced humidity also exhibited reduced growth”

✓ located verbatim in the source · abstract, characters 490–615 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Cauliflower plantlets cultured under reduced humidity developed large quantities of surface wax.

Treatment
cultured under reduced humiditye5
Outcome
surface waxe6
Direction
increaseinferrede6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e5

“Plantlets of cauliflower ( Brassica oleracea L. Botrytis group) cultured under reduced humidity also exhibited reduced growth”

✓ located verbatim in the source · abstract, characters 490–615 · show in text ↑
e6

“developed large quantities of surface wax”

✓ located verbatim in the source · abstract, characters 634–675 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Cauliflower plantlets cultured under reduced humidity had lower rates of water loss.

Treatment
cultured under reduced humiditye5
Outcome
rates of water losse7
Direction
lowere7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e5

“Plantlets of cauliflower ( Brassica oleracea L. Botrytis group) cultured under reduced humidity also exhibited reduced growth”

✓ located verbatim in the source · abstract, characters 490–615 · show in text ↑
e7

“had lower rates of water loss”

✓ located verbatim in the source · abstract, characters 680–709 · show in text ↑

humidity · survival · ChrysanthemumPositive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

humidity · growth · Brassica oleracea (wild cabbage)Positive and negative results

5 papers: 3 positive, 2 negative or no effect

1 paper in the same direction as this paper · 3 in the opposite direction

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

humidity · growth · ChrysanthemumResults agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.