Abstract
Abstract A method is described whereby relative humidity is reduced in the atmosphere of agar-solidified tissue cultures. Plantlets of chrysanthemum ( Chrysanthemum X morifolium Ramat.) cultured under low humidity exhibited a high mortality rate and those that survived were smaller and had few roots. Severe retardation of stomatal development occurred under reduced humidity and mature stomata had considerably narrower apertures than did plantlets grown at about 100% relative humidity. Plantlets of cauliflower ( Brassica oleracea L. Botrytis group) cultured under reduced humidity also exhibited reduced growth but, in addition, developed large quantities of surface wax and had lower rates of water loss. The results are discussed in relation to the potential uses of the technique for acclimatization of plantlets prior to transfer to soil.
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Claims 8
Claim · model-written summary
Chrysanthemum plantlets cultured under low humidity showed a high mortality rate.
- Taxon
- chrysanthemum
Chrysanthemume1 - Treatment
- cultured under low humiditye1
- Outcome
- mortality ratee1
- Direction
- increaseinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e1“Plantlets of chrysanthemum ( Chrysanthemum X morifolium Ramat.) cultured under low humidity exhibited a high mortality rate”
Claim · model-written summary
Chrysanthemum plantlets that survived low-humidity culture were smaller.
- Taxon
- chrysanthemum
Chrysanthemume1 - Treatment
- cultured under low humiditye1
- Outcome
- those that survivede2
- Direction
- smallere2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpeg?width=500)
Verbatim
e1“Plantlets of chrysanthemum ( Chrysanthemum X morifolium Ramat.) cultured under low humidity exhibited a high mortality rate”
e2“those that survived were smaller and had few roots”
Claim · model-written summary
Chrysanthemum plantlets that survived low-humidity culture had few roots.
- Taxon
- chrysanthemum
Chrysanthemume1 - Treatment
- cultured under low humiditye1
- Outcome
- rootse2
- Direction
- fewe2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpeg?width=500)
Verbatim
e1“Plantlets of chrysanthemum ( Chrysanthemum X morifolium Ramat.) cultured under low humidity exhibited a high mortality rate”
e2“those that survived were smaller and had few roots”
Claim · model-written summary
Reduced humidity severely retarded stomatal development in the tissue-cultured plantlets.
Not reportedTaxonComparator
Verbatim
e3“Severe retardation of stomatal development occurred under reduced humidity”
Claim · model-written summary
Mature stomata under reduced humidity had considerably narrower apertures than stomata of plantlets grown at about 100% relative humidity.
- Organ / stage
- mature stomatae4
- Treatment
- reduced humiditye3
- Outcome
- aperturese4
- Direction
- narrowere4
- Comparator
- plantlets grown at about 100% relative humiditye4
Not reportedTaxon
Verbatim
e3“Severe retardation of stomatal development occurred under reduced humidity”
e4“mature stomata had considerably narrower apertures than did plantlets grown at about 100% relative humidity”
Claim · model-written summary
Cauliflower plantlets cultured under reduced humidity showed reduced growth.
- Taxon
- cauliflower
Brassica oleracea var. botrytise5 - Treatment
- cultured under reduced humiditye5
- Outcome
- growthe5
- Direction
- reducede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e5“Plantlets of cauliflower ( Brassica oleracea L. Botrytis group) cultured under reduced humidity also exhibited reduced growth”
Claim · model-written summary
Cauliflower plantlets cultured under reduced humidity developed large quantities of surface wax.
- Taxon
- cauliflower
Brassica oleracea var. botrytise5 - Treatment
- cultured under reduced humiditye5
- Outcome
- surface waxe6
- Direction
- increaseinferrede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e5“Plantlets of cauliflower ( Brassica oleracea L. Botrytis group) cultured under reduced humidity also exhibited reduced growth”
e6“developed large quantities of surface wax”
Claim · model-written summary
Cauliflower plantlets cultured under reduced humidity had lower rates of water loss.
- Taxon
- cauliflower
Brassica oleracea var. botrytise5 - Treatment
- cultured under reduced humiditye5
- Outcome
- rates of water losse7
- Direction
- lowere7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e5“Plantlets of cauliflower ( Brassica oleracea L. Botrytis group) cultured under reduced humidity also exhibited reduced growth”
e7“had lower rates of water loss”
The same questions in other papers
humidity · survival · ChrysanthemumPositive and negative results
1 in the opposite direction
- positive
humidity · growth · Brassica oleracea (wild cabbage)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →humidity · growth · ChrysanthemumResults agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.