Abstract
Salt stress seriously reduced the yield and quality of watermelon and restricted the sustainable development of the watermelon industry. However, the molecular mechanism of watermelon in response to salt stress is still unclear. In this study, 150 mmol·L−1 NaCl was used to deal with the seedlings of salt-tolerant and salt-sensitive watermelon varieties. Physiological characteristics showed that salt stress significantly reduced the biomass of watermelon seedlings and the accumulation of K+ in roots and leaves and significantly increased the content of Na+, Cl−, and malondialdehyde (MDA). Compared with the salt-sensitive variety, the salt-tolerant variety had higher K+ accumulation, lower Cl−, Cl− accumulation, and MDA content in roots and leaves. Then, RNA-seq was performed on roots and leaves in normal culture and under 150 mmol·L−1 NaCl treatment. A total of 21,069 genes were identified by RNA-seq analysis, of which 1412 were genes encoding transcription factors (TFs). In the comparison groups of roots and leaves, 122 and 123 shared differentially expressed genes (DEGs) were obtained, respectively. Gene ontology (GO) annotation and KEGG enrichment results showed that there were many identical GO terms and KEGG pathways in roots and leaves, especially the pathways that related to sugar or energy (ATP or NADP+/NADPH). In addition, some DEGs related to salt tolerance were identified, such as plant hormone indole-3-acetic acid (IAA) and gibberellin (GA) signal transduction pathway-related genes, K+/Na+/Ca2+-related genes, lignin biosynthesis-related genes, etc. At the same time, we also identified some TFs related to salt tolerance, such as AP2-EREBP, bZIP, bHLH, MYB, NAC, OFP, TCP, and WRKY and found that these TFs had high correlation coefficients with salt tolerance-related genes, indicating that they might have a potential regulatory relationship. Interestingly, one TCP TF (Cla97C09G174040) co-exists both in roots and leaves, and it is speculated that it may be regulated by miR319 to improve the salt tolerance of watermelon.
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Claims 12
Claim · model-written summary
Salt stress significantly reduced seedling biomass and root and leaf K+ accumulation.
- Taxon
- Watermelon
Citrullus lanatust0 - Treatment
- salt stresse0
- Outcome
- biomass of watermelon seedlings and the accumulation of K+ in roots and leavese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus)”
e0“salt stress significantly reduced the biomass of watermelon seedlings and the accumulation of K+ in roots and leaves”
Claim · model-written summary
Salt stress significantly increased Na+, Cl− and MDA contents.
- Taxon
- WatermelonCitrullus lanatust0
- Treatment
- salt stresse1
- Outcome
- content of Na+, Cl−, and malondialdehyde (MDA)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus)”
e1“salt stress significantly reduced the biomass of watermelon seedlings and the accumulation of K+ in roots and leaves”
e0“significantly increased the content of Na+, Cl−, and malondialdehyde (MDA)”
Claim · model-written summary
The salt-tolerant variety had higher K+ accumulation than the sensitive variety.
- Taxon
- WatermelonCitrullus lanatust0
- Material
- salt-tolerant varietye0
- Outcome
- K+ accumulatione0
- Direction
- highere0
- Comparator
- salt-sensitive varietye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus)”
e0“Compared with the salt-sensitive variety, the salt-tolerant variety had higher K+ accumulation”
Claim · model-written summary
The tolerant variety had lower Cl− accumulation and MDA content in roots and leaves.
- Taxon
- WatermelonCitrullus lanatust0
- Material
- salt-tolerant varietye0
- Outcome
- Cl− accumulation, and MDA contente0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus)”
e0“the salt-tolerant variety had higher K+ accumulation, lower Cl−, Cl− accumulation, and MDA content in roots and leaves.”
Claim · model-written summary
RNA sequencing identified 21,069 genes, including 1412 encoding transcription factors.
- Taxon
- WatermelonCitrullus lanatust0
- Outcome
- genese0
- Value
- 21,069 genese0
- Qualifier
- 1412 were genes encoding transcription factors (TFs)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus)”
e0“A total of 21,069 genes were identified by RNA-seq analysis, of which 1412 were genes encoding transcription factors (TFs).”
Claim · model-written summary
Roots had 122 shared differentially expressed genes.
- Taxon
- WatermelonCitrullus lanatust0
- Organ / stage
- rootse0
- Outcome
- shared differentially expressed genes (DEGs)e0
- Value
- 122e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus)”
e0“In the comparison groups of roots and leaves, 122 and 123 shared differentially expressed genes (DEGs) were obtained, respectively.”
Claim · model-written summary
Leaves had 123 shared differentially expressed genes.
- Taxon
- WatermelonCitrullus lanatust0
- Organ / stage
- leavese0
- Outcome
- shared differentially expressed genes (DEGs)e0
- Value
- 123e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus)”
e0“In the comparison groups of roots and leaves, 122 and 123 shared differentially expressed genes (DEGs) were obtained, respectively.”
Claim · model-written summary
Root and leaf analyses shared many GO terms and KEGG pathways, especially sugar and energy pathways.
- Taxon
- WatermelonCitrullus lanatust0
- Organ / stage
- roots and leavese0
- Outcome
- identical GO terms and KEGG pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus)”
e0“there were many identical GO terms and KEGG pathways in roots and leaves, especially the pathways that related to sugar or energy (ATP or NADP+/NADPH).”
Claim · model-written summary
The study identified DEGs related to hormone signaling, ion relations and lignin biosynthesis.
- Taxon
- WatermelonCitrullus lanatust0
- Outcome
- DEGs related to salt tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus)”
e0“some DEGs related to salt tolerance were identified, such as plant hormone indole-3-acetic acid (IAA) and gibberellin (GA) signal transduction pathway-related genes, K+/Na+/Ca2+-related genes, lignin biosynthesis-related genes”
Claim · model-written summary
Several transcription factor families correlated strongly with salt-tolerance genes.
- Taxon
- WatermelonCitrullus lanatust0
- Gene
- AP2-EREBPe0
- Outcome
- high correlation coefficients with salt tolerance-related genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus)”
e0“TFs related to salt tolerance, such as AP2-EREBP, bZIP, bHLH, MYB, NAC, OFP, TCP, and WRKY and found that these TFs had high correlation coefficients with salt tolerance-related genes”
Claim · model-written summary
One TCP transcription factor occurred in both roots and leaves.
- Taxon
- WatermelonCitrullus lanatust0
- Gene
- Cla97C09G174040e0
- Outcome
- co-exists both in roots and leavese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus)”
e0“one TCP TF (Cla97C09G174040) co-exists both in roots and leaves”
Claim · model-written summary
The authors speculate that miR319 regulates Cla97C09G174040 to improve salt tolerance.
- Taxon
- WatermelonCitrullus lanatust0
- Gene
- miR319e0
- Outcome
- salt tolerance of watermelone0
- Qualifier
- it is speculatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus)”
e0“it is speculated that it may be regulated by miR319 to improve the salt tolerance of watermelon.”
The same questions in other papers
salinity · physiology: oxidative · Citrullus lanatus (watermelon)Positive and negative results
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- positive
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1 paper more in this group
See all 5 papers →salinity · physiology: ion balance · Citrullus lanatus (watermelon)Results agree
3 papers in the same direction as this paper
- negative
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.