Abstract
Heat shock transcription factor (Hsf) exists widely in eukaryotes and responds to various abiotic stresses by regulating the expression of downstream transcription factors, functional enzymes, and molecular chaperones. In this study, TaHsfA2-13 , a heat shock transcription factor belonging to A2 subclass, was cloned from wheat ( Triticum aestivum ) and its function was analyzed. TaHsfA2-13 encodes a protein containing 368 amino acids and has the basic characteristics of Hsfs. Multiple sequence alignment analysis showed that TaHsfA2-13 protein had the highest similarity with TdHsfA2c-like protein from Triticum dicoccoides , which reached 100%. The analysis of tissue expression characteristics revealed that TaHsfA2-13 was highly expressed in root, shoot, and leaf during the seedling stage of wheat. The expression of TaHsfA2-13 could be upregulated by heat stress, low temperature, H 2 O 2 , mannitol, salinity and multiple phytohormones. The TaHsfA2-13 protein was located in the nucleus under the normal growth conditions and showed a transcriptional activation activity in yeast. Further studies found that overexpression of TaHsfA2-13 in Arabidopsis thaliana Col-0 or athsfa2 mutant results in improved tolerance to heat stress, H 2 O 2 , SA and mannitol by regulating the expression of multiple heat shock protein ( Hsp ) genes. In summary, our study identified TaHsfA2-13 from wheat, revealed its regulatory function in varieties of abiotic stresses, and will provide a new target gene to improve stress tolerance for wheat breeding.
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Claims 8
Claim · model-written summary
Wheat TaHsfA2-13 encodes a 368-amino-acid heat shock factor.
- Taxon
- Wheat
Triticum aestivumt0 - Gene
- TaHsfA2-13e0
- Outcome
- protein containing 368 amino acidse0
- Value
- 368 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaHsfA2-13 , a heat shock transcription factor belonging to A2 subclass, was cloned from wheat ( Triticum aestivum ) and its function was analyzed. TaHsfA2-13 encodes a protein containing 368 amino acids and has the basic characteristics of Hsfs.”
t0“Wheat”
Claim · model-written summary
TaHsfA2-13 protein had 100% similarity to TdHsfA2c-like protein.
- Taxon
- WheatTriticum aestivumt0
- Gene
- TaHsfA2-13 proteine0
- Outcome
- similarity with TdHsfA2c-like proteine0
- Direction
- higheste0
- Value
- 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaHsfA2-13 protein had the highest similarity with TdHsfA2c-like protein from Triticum dicoccoides , which reached 100%.”
t0“Wheat”
Claim · model-written summary
TaHsfA2-13 expression was high in wheat seedling root, shoot, and leaf.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- seedling stagee0
- Gene
- TaHsfA2-13e0
- Outcome
- expressed in root, shoot, and leafe0
- Direction
- highlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaHsfA2-13 was highly expressed in root, shoot, and leaf during the seedling stage of wheat.”
t0“Wheat”
Claim · model-written summary
Heat, cold, hydrogen peroxide, mannitol, salinity, and phytohormones upregulated TaHsfA2-13 expression.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- heat stress, low temperature, H 2 O 2 , mannitol, salinity and multiple phytohormonese0
- Gene
- TaHsfA2-13e0
- Outcome
- expression of TaHsfA2-13e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of TaHsfA2-13 could be upregulated by heat stress, low temperature, H 2 O 2 , mannitol, salinity and multiple phytohormones.”
t0“Wheat”
Claim · model-written summary
TaHsfA2-13 protein localized to nuclei under normal conditions.
- Taxon
- WheatTriticum aestivumt0
- Gene
- TaHsfA2-13 proteine0
- Outcome
- located in the nucleuse0
- Qualifier
- normal growth conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The TaHsfA2-13 protein was located in the nucleus under the normal growth conditions and showed a transcriptional activation activity in yeast.”
t0“Wheat”
Claim · model-written summary
TaHsfA2-13 had transcriptional activation activity in yeast.
- Taxon
- WheatTriticum aestivumt0
- Gene
- TaHsfA2-13 proteine0
- Outcome
- transcriptional activation activity in yeaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The TaHsfA2-13 protein was located in the nucleus under the normal growth conditions and showed a transcriptional activation activity in yeast.”
t0“Wheat”
Claim · model-written summary
Overexpression of wheat TaHsfA2-13 improved Arabidopsis tolerance to heat, hydrogen peroxide, SA, and mannitol.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Material
- Col-0 or athsfa2 mutante0
- Treatment
- overexpression of TaHsfA2-13e0
- Gene
- TaHsfA2-13e0
- Outcome
- tolerance to heat stress, H 2 O 2 , SA and mannitole0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of TaHsfA2-13 in Arabidopsis thaliana Col-0 or athsfa2 mutant results in improved tolerance to heat stress, H 2 O 2 , SA and mannitol by regulating the expression of multiple heat shock protein ( Hsp ) genes.”
Claim · model-written summary
TaHsfA2-13 overexpression regulated multiple heat shock protein genes in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- overexpression of TaHsfA2-13e0
- Gene
- TaHsfA2-13e0
- Outcome
- expression of multiple heat shock protein ( Hsp ) genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of TaHsfA2-13 in Arabidopsis thaliana Col-0 or athsfa2 mutant results in improved tolerance to heat stress, H 2 O 2 , SA and mannitol by regulating the expression of multiple heat shock protein ( Hsp ) genes.”
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