Abstract
Abiotic stresses are major constraints limiting crop growth and production. Heat shock factors (Hsfs) play significant roles in mediating plant resistance to various environmental stresses, including heat, drought and salinity. In this study, we explored the biological functions and underlying mechanisms of wheat TaHsfA6f in plant tolerance to various abiotic stresses. Gene expression profiles showed that TaHsfA6f has relatively high expression levels in wheat leaves at the reproductive stage. Transcript levels of TaHsfA6f were substantially up-regulated by heat, dehydration, salinity, low temperature, and multiple phytohormones, but was not induced by brassinosteroids (BR). Subcellular localization analyses revealed that TaHsfA6f is localized to the nucleus. Overexpression of the TaHsfA6f gene in Arabidopsis results in improved tolerance to heat, drought and salt stresses, enhanced sensitivity to exogenous abscisic acid (ABA), and increased accumulation of ABA. Furthermore, RNA-sequencing data demonstrated that TaHsfA6f functions through up-regulation of a number of genes involved in ABA metabolism and signaling, and other stress-associated genes. Collectively, these results provide evidence that TaHsfA6f participates in the regulation of multiple abiotic stresses, and that TaHsfA6f could serve as a valuable gene for genetic modification of crop abiotic stress tolerance.
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Claims 9
Claim · model-written summary
TaHsfA6f expression was relatively high in reproductive-stage wheat leaves.
- Taxon
- wheat
Triticum aestivume0 - Organ / stage
- leaves at the reproductive stagee0
- Gene
- TaHsfA6fe0
- Outcome
- expression levelse0
- Direction
- relatively highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaHsfA6f has relatively high expression levels in wheat leaves at the reproductive stage.”
Claim · model-written summary
Heat, dehydration, salinity, low temperature and multiple phytohormones up-regulated TaHsfA6f transcripts.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- heat, dehydration, salinity, low temperature, and multiple phytohormonese0
- Gene
- TaHsfA6fe0
- Outcome
- Transcript levels of TaHsfA6fe0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcript levels of TaHsfA6f were substantially up-regulated by heat, dehydration, salinity, low temperature, and multiple phytohormones”
t0“Wheat”
Claim · model-written summary
Brassinosteroids did not induce TaHsfA6f transcripts.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- brassinosteroidse0
- Gene
- TaHsfA6ft0
- Outcome
- Transcript levels of TaHsfA6fe1
- Direction
- not inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but was not induced by brassinosteroids (BR).”
t0“Wheat Heat Shock Factor TaHsfA6f”
e1“Transcript levels of TaHsfA6f”
Claim · model-written summary
TaHsfA6f localized to the nucleus.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaHsfA6fe0
- Outcome
- localized to the nucleuse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Subcellular localization analyses revealed that TaHsfA6f is localized to the nucleus.”
t0“Wheat”
Claim · model-written summary
TaHsfA6f overexpression improved Arabidopsis tolerance to heat, drought and salt.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Overexpression of the TaHsfA6f genee0
- Gene
- TaHsfA6fe0
- Outcome
- tolerance to heat, drought and salt stressese0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of the TaHsfA6f gene in Arabidopsis results in improved tolerance to heat, drought and salt stresses”
Claim · model-written summary
TaHsfA6f overexpression enhanced Arabidopsis sensitivity to exogenous ABA.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Overexpression of the TaHsfA6f genee0
- Gene
- TaHsfA6fe0
- Outcome
- sensitivity to exogenous abscisic acid (ABA)e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of the TaHsfA6f gene in Arabidopsis results in improved tolerance to heat, drought and salt stresses, enhanced sensitivity to exogenous abscisic acid (ABA), and increased accumulation of ABA.”
Claim · model-written summary
TaHsfA6f overexpression increased ABA accumulation in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Overexpression of the TaHsfA6f genee0
- Gene
- TaHsfA6fe0
- Outcome
- accumulation of ABAe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of the TaHsfA6f gene in Arabidopsis results in improved tolerance to heat, drought and salt stresses, enhanced sensitivity to exogenous abscisic acid (ABA), and increased accumulation of ABA.”
Claim · model-written summary
RNA sequencing indicated TaHsfA6f up-regulates genes for ABA metabolism, signaling and other stress responses.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaHsfA6fe0
- Outcome
- up-regulation of a number of genes involved in ABA metabolism and signaling, and other stress-associated genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-sequencing data demonstrated that TaHsfA6f functions through up-regulation of a number of genes involved in ABA metabolism and signaling, and other stress-associated genes.”
t0“Wheat”
Claim · model-written summary
The authors propose TaHsfA6f for genetic modification of crop abiotic stress tolerance.
Verbatim
e0“TaHsfA6f could serve as a valuable gene for genetic modification of crop abiotic stress tolerance.”
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