Abstract
BACKGROUND: NAC (NAM, ATAF, and CUC) transcription factors play important roles in plant biological processes, including phytohormone homeostasis, plant development, and in responses to various environmental stresses. METHODS: TaNAC29 was introduced into Arabidopsis using the Agrobacterium tumefaciens-mediated floral dipping method. TaNAC29-overexpression plants were subjected to salt and drought stresses for examining gene functions. To investigate tolerant mechanisms involved in the salt and drought responses, expression of related marker genes analyses were conducted, and related physiological indices were also measured. Expressions of genes were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: A novel NAC transcription factor gene, designated TaNAC29, was isolated from bread wheat (Triticum aestivum). Sequence alignment suggested that TaNAC29 might be located on chromosome 2BS. TaNAC29 was localized to the nucleus in wheat protoplasts, and proved to have transcriptional activation activities in yeast. TaNAC29 was expressed at a higher level in the leaves, and expression levels were much higher in senescent leaves, indicating that TaNAC29 might be involved in the senescence process. TaNAC29 transcripts were increased following treatments with salt, PEG6000, H2O2, and abscisic acid (ABA). To examine TaNAC29 function, transgenic Arabidopsis plants overexpressing TaNAC29 were generated. Germination and root length assays of transgenic plants demonstrated that TaNAC29 overexpression plants had enhanced tolerances to high salinity and dehydration, and exhibited an ABA-hypersensitive response. When grown in the greenhouse, TaNAC29-overexpression plants showed the same tolerance response to salt and drought stresses at both the vegetative and reproductive period, and had delayed bolting and flowering in the reproductive period. Moreover, TaNAC29 overexpression plants accumulated lesser malondialdehyde (MDA), H2O2, while had higher superoxide dismutase (SOD) and catalase (CAT) activities under high salinity and/or dehydration stress. CONCLUSIONS: Our results demonstrate that TaNAC29 plays important roles in the senescence process and response to salt and drought stresses. ABA signal pathway and antioxidant enzyme systems are involved in TaNAC29-mediated stress tolerance mechanisms.
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Claims 13
Claim · model-written summary
TaNAC29 was isolated from bread wheat.
- Taxon
- bread wheat (Triticum aestivum)
Triticum aestivume0 - Gene
- TaNAC29e0
- Outcome
- was isolatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A novel NAC transcription factor gene, designated TaNAC29, was isolated from bread wheat (Triticum aestivum)”
Claim · model-written summary
Sequence alignment suggested TaNAC29 lies on chromosome 2BS.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaNAC29e0
- Outcome
- located on chromosome 2BSe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequence alignment suggested that TaNAC29 might be located on chromosome 2BS”
t0“from wheat”
Claim · model-written summary
TaNAC29 localized to the nucleus in wheat protoplasts.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaNAC29e0
- Outcome
- localized to the nucleuse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC29 was localized to the nucleus in wheat protoplasts”
Claim · model-written summary
TaNAC29 showed transcriptional activation in yeast.
Not reportedDirection
Verbatim
e0“TaNAC29 was localized to the nucleus in wheat protoplasts, and proved to have transcriptional activation activities in yeast”
Claim · model-written summary
TaNAC29 expression was higher in leaves and much higher in senescent leaves.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- senescent leavese0
- Gene
- TaNAC29e0
- Outcome
- expression levelse0
- Direction
- much highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC29 was expressed at a higher level in the leaves, and expression levels were much higher in senescent leaves”
t0“from wheat”
Claim · model-written summary
Salt, PEG6000, H2O2 and ABA increased TaNAC29 transcripts.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- salt, PEG6000, H2O2, and abscisic acid (ABA)e0
- Gene
- TaNAC29e0
- Outcome
- TaNAC29 transcriptse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC29 transcripts were increased following treatments with salt, PEG6000, H2O2, and abscisic acid (ABA)”
t0“from wheat”
Claim · model-written summary
TaNAC29 overexpression enhanced Arabidopsis tolerance to high salinity and dehydration.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- TaNAC29 overexpressione0
- Gene
- TaNAC29e0
- Outcome
- tolerances to high salinity and dehydratione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC29 overexpression plants had enhanced tolerances to high salinity and dehydration”
t0“in transgenic Arabidopsis”
Claim · model-written summary
TaNAC29 overexpression produced an ABA hypersensitive response.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- TaNAC29 overexpressione0
- Outcome
- ABA-hypersensitive responsee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC29 overexpression plants had enhanced tolerances to high salinity and dehydration, and exhibited an ABA-hypersensitive response”
t0“in transgenic Arabidopsis”
Claim · model-written summary
Greenhouse TaNAC29 overexpression plants tolerated salt and drought during vegetative and reproductive periods.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- vegetative and reproductive periode0
- Treatment
- TaNAC29-overexpressione0
- Outcome
- tolerance response to salt and drought stressese0
- Setting
- greenhousee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When grown in the greenhouse, TaNAC29-overexpression plants showed the same tolerance response to salt and drought stresses at both the vegetative and reproductive period”
t0“in transgenic Arabidopsis”
Claim · model-written summary
TaNAC29 overexpression delayed bolting and flowering.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- reproductive periode0
- Treatment
- transgenict0
- Gene
- TaNAC29t0
- Outcome
- bolting and floweringe0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“had delayed bolting and flowering in the reproductive period”
t0“TaNAC29, a NAC transcription factor from wheat, enhances salt and drought tolerance in transgenic Arabidopsis”
Claim · model-written summary
Under salinity or dehydration, overexpression plants accumulated less MDA and H2O2.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- TaNAC29 overexpressione0
- Outcome
- malondialdehyde (MDA), H2O2e0
- Direction
- lessere0
- Qualifier
- under high salinity and/or dehydration stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC29 overexpression plants accumulated lesser malondialdehyde (MDA), H2O2, while had higher superoxide dismutase (SOD) and catalase (CAT) activities under high salinity and/or dehydration stress”
t0“in transgenic Arabidopsis”
Claim · model-written summary
Under salinity or dehydration, overexpression plants had higher SOD and CAT activities.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- TaNAC29 overexpressione0
- Outcome
- superoxide dismutase (SOD) and catalase (CAT) activitiese0
- Direction
- highere0
- Qualifier
- under high salinity and/or dehydration stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC29 overexpression plants accumulated lesser malondialdehyde (MDA), H2O2, while had higher superoxide dismutase (SOD) and catalase (CAT) activities under high salinity and/or dehydration stress”
t0“in transgenic Arabidopsis”
Claim · model-written summary
Authors implicated ABA signaling and antioxidant enzymes in TaNAC29-mediated tolerance.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- TaNAC29e0
- Outcome
- stress tolerance mechanismse0
- Qualifier
- ABA signal pathway and antioxidant enzyme systemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA signal pathway and antioxidant enzyme systems are involved in TaNAC29-mediated stress tolerance mechanisms”
t0“in transgenic Arabidopsis”
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