Abstract
Abstract Culture conditions, allowing the unlimited growth and maintenance in shaken suspensions of a kinetin dependent strain of tobacco pith cells, were determined. Cell clusters from 1 to 25 cells were selected from the cultures to study the cell multiplication after addition of specific growth factors to the basal medium. Cell division was found to be strictly dependent upon the presence in the medium of both kinetin and auxin. In complete medium the generation period was 45 to 49 hours. Kinetics of the total protein synthesis in the cell suspensions have been measured either by estimation of protein nitrogen or by 35 S incorporation into the proteins. After 3 days of culture, growth was exponential, as expressed by cell number or protein synthesis, which varied proportionally. Evidence was also found that the initial incorporation rate of radioactivity into the proteins was more rapid than expected from the increment of the protein net weight. It seems therefore reasonable to assume that a significant amount of protein turn over does exist during the initial period of growth. This phenomenon was observed even when no cell division occurred. Kinetin significantly activated protein synthesis, whether or not auxin was present in the medium.
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Claims 6
Claim · model-written summary
Tobacco cell division required both kinetin and auxin.
- Taxon
- tobacco
Nicotiana tabacumt0 - Treatment
- both kinetin and auxine0
- Outcome
- Cell divisione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cell division was found to be strictly dependent upon the presence in the medium of both kinetin and auxin”
t0“tobacco pith cells”
Claim · model-written summary
Generation period in complete medium was 45 to 49 hours.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- generation periode0
- Value
- 45 to 49 hourse0
- Qualifier
- complete mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In complete medium the generation period was 45 to 49 hours”
t0“tobacco pith cells”
Claim · model-written summary
After three days, cell number and protein synthesis grew exponentially and proportionally.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- cell number or protein synthesise0
- Direction
- exponentiale0
- Qualifier
- After 3 days of culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 3 days of culture, growth was exponential, as expressed by cell number or protein synthesis, which varied proportionally”
t0“tobacco pith cells”
Claim · model-written summary
Initial radioactive incorporation into proteins was faster than protein net weight gain.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- initial incorporation rate of radioactivity into the proteinse0
- Direction
- more rapide0
- Comparator
- increment of the protein net weighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“initial incorporation rate of radioactivity into the proteins was more rapid than expected from the increment of the protein net weight”
t0“tobacco pith cells”
Claim · model-written summary
Protein turnover was inferred during initial growth even without cell division.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- protein turn overe0
- Qualifier
- initial period of growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a significant amount of protein turn over does exist during the initial period of growth. This phenomenon was observed even when no cell division occurred”
t0“tobacco pith cells”
Claim · model-written summary
Kinetin significantly activated protein synthesis with or without auxin.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Kinetine0
- Outcome
- protein synthesise0
- Direction
- activatede0
- Qualifier
- whether or not auxin was present in the mediume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Kinetin significantly activated protein synthesis, whether or not auxin was present in the medium”
t0“tobacco pith cells”
The same questions in other papers
kinetin · quality: protein · Nicotiana tabacum (tobacco)Results differ
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.