Abstract
The rice sucrose synthase 1 (RSus1) gene is transcriptionally induced by sucrose, and a region within its promoter, at -1117 to -958 upstream of the transcription initiation site, was found to be essential for enhancing the sucrose-induced expression. Further dissection of this region revealed that a group of nuclear proteins interact with a 39-bp fragment named A-3-2 (-1045 to -1007). A protein that specifically and directly interacted with A-3-2 was isolated from the suspension-cultured cells of rice and was subsequently identified as a purine-rich DNA-binding protein. The amino acid sequence of this protein, OsPurα, exhibited 73% identity with the Arabidopsis Purα-1 protein, and its modeled structure resembled the structure of Pur-α in Drosophila. Recombinant OsPurα expressed and purified from Escherichia coli was demonstrated to have DNA-binding activity and to interact with A-3-2 specifically. Moreover, OsPurα was able to enhance sucrose-induced expression of the β-glucuronidase (GUS) reporter gene, which was transcriptionally fused to two copies of a DNA fragment containing A-3-2 and the cauliflower mosaic virus 35S minimal promoter, in vivo. The level of OsPurα bound to A-3-2 was higher in cells cultured in the presence of sucrose; however, the level of OsPurα mRNA in cells was not affected by sucrose. The results of this study demonstrate that OsPurα participates in the regulation of RSus1 expression in response to sucrose; nevertheless, it may require other partner proteins for full function.
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Claims 10
Claim · model-written summary
A promoter region at -1117 to -958 is essential for sucrose-induced expression of the rice RSus1 gene.
- Taxon
- rice
Oryza sativae1 - Gene
- RSus1e1
- Outcome
- sucrose-induced expressione1
- Qualifier
- a region within its promoter, at -1117 to -958 upstream of the transcription initiation sitee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice sucrose synthase 1 (RSus1) gene is transcriptionally induced by sucrose, and a region within its promoter, at -1117 to -958 upstream of the transcription initiation site, was found to be essential for enhancing the sucrose-induced expression”
Claim · model-written summary
A purine-rich DNA-binding protein from rice suspension-cultured cells specifically and directly interacted with A-3-2.
- Taxon
- riceOryza sativae2
- Material
- suspension-cultured cellse2
- Outcome
- specifically and directly interacted with A-3-2e2
- Qualifier
- identified as a purine-rich DNA-binding proteine2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“A protein that specifically and directly interacted with A-3-2 was isolated from the suspension-cultured cells of rice and was subsequently identified as a purine-rich DNA-binding protein”
Claim · model-written summary
OsPurα shares 73% amino acid sequence identity with the Arabidopsis Purα-1 protein.
- Taxon
- riceOryza sativae1
- Gene
- OsPurαe3
- Outcome
- amino acid sequencee3
- Value
- 73%e3
- Comparator
- Arabidopsis Purα-1 proteine3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice sucrose synthase 1 (RSus1) gene is transcriptionally induced by sucrose, and a region within its promoter, at -1117 to -958 upstream of the transcription initiation site, was found to be essential for enhancing the sucrose-induced expression”
e3“The amino acid sequence of this protein, OsPurα, exhibited 73% identity with the Arabidopsis Purα-1 protein”
Claim · model-written summary
The modeled structure of OsPurα resembles Pur-α in Drosophila.
- Taxon
- riceOryza sativae1
- Outcome
- modeled structuree4
- Comparator
- Pur-α in Drosophilae4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice sucrose synthase 1 (RSus1) gene is transcriptionally induced by sucrose, and a region within its promoter, at -1117 to -958 upstream of the transcription initiation site, was found to be essential for enhancing the sucrose-induced expression”
e4“its modeled structure resembled the structure of Pur-α in Drosophila”
Claim · model-written summary
Recombinant OsPurα has DNA-binding activity.
- Taxon
- riceOryza sativae1
- Gene
- OsPurαe5
- Outcome
- DNA-binding activitye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice sucrose synthase 1 (RSus1) gene is transcriptionally induced by sucrose, and a region within its promoter, at -1117 to -958 upstream of the transcription initiation site, was found to be essential for enhancing the sucrose-induced expression”
e5“Recombinant OsPurα expressed and purified from Escherichia coli was demonstrated to have DNA-binding activity and to interact with A-3-2 specifically”
Claim · model-written summary
Recombinant OsPurα interacts with A-3-2 specifically.
- Taxon
- riceOryza sativae1
- Gene
- OsPurαe5
- Outcome
- interact with A-3-2 specificallye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice sucrose synthase 1 (RSus1) gene is transcriptionally induced by sucrose, and a region within its promoter, at -1117 to -958 upstream of the transcription initiation site, was found to be essential for enhancing the sucrose-induced expression”
e5“Recombinant OsPurα expressed and purified from Escherichia coli was demonstrated to have DNA-binding activity and to interact with A-3-2 specifically”
Claim · model-written summary
OsPurα enhanced sucrose-induced expression of a GUS reporter fused to A-3-2 in vivo.
- Taxon
- riceOryza sativae1
- Treatment
- OsPurαe6
- Outcome
- sucrose-induced expressione6
- Direction
- enhancee6
- Setting
- in vivoe6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice sucrose synthase 1 (RSus1) gene is transcriptionally induced by sucrose, and a region within its promoter, at -1117 to -958 upstream of the transcription initiation site, was found to be essential for enhancing the sucrose-induced expression”
e6“OsPurα was able to enhance sucrose-induced expression of the β-glucuronidase (GUS) reporter gene, which was transcriptionally fused to two copies of a DNA fragment containing A-3-2 and the cauliflower mosaic virus 35S minimal promoter, in vivo”
Claim · model-written summary
The level of OsPurα bound to A-3-2 was higher in cells cultured with sucrose.
- Taxon
- riceOryza sativae1
- Treatment
- in the presence of sucrosee7
- Outcome
- The level of OsPurα bound to A-3-2e7
- Direction
- highere7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice sucrose synthase 1 (RSus1) gene is transcriptionally induced by sucrose, and a region within its promoter, at -1117 to -958 upstream of the transcription initiation site, was found to be essential for enhancing the sucrose-induced expression”
e7“The level of OsPurα bound to A-3-2 was higher in cells cultured in the presence of sucrose”
Claim · model-written summary
Sucrose did not affect OsPurα mRNA level in cells.
- Taxon
- riceOryza sativae1
- Treatment
- sucrosee7
- Outcome
- the level of OsPurα mRNA in cellse7
- Direction
- not affectede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice sucrose synthase 1 (RSus1) gene is transcriptionally induced by sucrose, and a region within its promoter, at -1117 to -958 upstream of the transcription initiation site, was found to be essential for enhancing the sucrose-induced expression”
e7“the level of OsPurα mRNA in cells was not affected by sucrose”
Claim · model-written summary
OsPurα participates in regulating RSus1 expression in response to sucrose, possibly with other partner proteins.
- Taxon
- riceOryza sativae1
- Gene
- OsPurαe6
- Outcome
- regulation of RSus1 expressione6
- Qualifier
- it may require other partner proteins for full functione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice sucrose synthase 1 (RSus1) gene is transcriptionally induced by sucrose, and a region within its promoter, at -1117 to -958 upstream of the transcription initiation site, was found to be essential for enhancing the sucrose-induced expression”
e6“OsPurα participates in the regulation of RSus1 expression in response to sucrose; nevertheless, it may require other partner proteins for full function”
The same questions in other papers
sucrose · molecular: expression · Oryza sativa (rice)Positive and negative results
3 papers in the same direction as this paper · 14 in the opposite direction
- positive
- positive
- positive
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.