Abstract
The present paper deals with the factors affecting the induction, formation, dif- ferentiation and the frequency of induction of rice pollen plantlets cultured in vitro. We have obtained the following results. 1. If the suitable stage of the development of pollen (late uninuclear stage at the verge of first mitosis) is ascertained, pollen callus may be obtained on a simple medium containing 2,4-D (2mg/1) and sucrose (6%). The gorwth substance, the source of carbon and the osmotic pressure are the necessary conditions for triggering the first division of the pollen. The complex medium consisting of minerals, trace elements, vitamins, sucrose and growth substances is not necessary for the initiation and the early growth of pollen callus, but all these factors in the medium are beneficial for the further deve- lopment of the callus. 2. Sucrose in the medium plays a significant role in the regulation of osmotic pressure. Excessively high concentration of sucrose inhibits callus formation. 3. Coconut milk, casein hydrolysate, mononucleotide and some other organic sup- plements are obviously beneficial to callus formation and the subsequent differentia- tion of the organs. 4. If the culture medium is supplemented with 2-chlorethvl-phosphonic acid (8 ppm, 40 ppm) or when the raceme of the rice is pretreated at 10℃, for 48 hours, the frequency of the formation of the pollen callus will be greatly promoted. 5. The age of the pollen callus affects the ability of the organ differentiation; the older the callus is, the lower the induction frequency of the pollen plantlets. The causes and the mechnisms of the above mentioned results have been dis- cussed.
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Claims 11
Claim · model-written summary
Late uninuclear pollen can form callus on a simple medium with 2,4-D and sucrose.
- Taxon
- RICE
Oryza sativat0 - Organ / stage
- pollene0
- Treatment
- simple medium containing 2,4-D (2mg/1) and sucrose (6%)e0
- Outcome
- pollen callus may be obtainede0
- Qualifier
- late uninuclear stage at the verge of first mitosise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“If the suitable stage of the development of pollen (late uninuclear stage at the verge of first mitosis) is ascertained, pollen callus may be obtained on a simple medium containing 2,4-D (2mg/1) and sucrose (6%).”
t0“ON THE CONDITIONS FOR THE INDUCTION OF RICE POLLEN PLANTLETS AND CERTAIN FACTORS AFFECTING THE FREQUENCY OF INDUCTION”
Claim · model-written summary
Growth substance, carbon source and osmotic pressure were necessary for the first pollen division.
- Taxon
- RICEOryza sativat0
- Outcome
- first division of the pollene0
- Qualifier
- The gorwth substance, the source of carbon and the osmotic pressuree0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gorwth substance, the source of carbon and the osmotic pressure are the necessary conditions for triggering the first division of the pollen.”
t0“ON THE CONDITIONS FOR THE INDUCTION OF RICE POLLEN PLANTLETS AND CERTAIN FACTORS AFFECTING THE FREQUENCY OF INDUCTION”
Claim · model-written summary
Complex medium was unnecessary for callus initiation and early growth.
- Taxon
- RICEOryza sativat0
- Treatment
- complex medium consisting of minerals, trace elements, vitamins, sucrose and growth substancese0
- Outcome
- initiation and the early growth of pollen calluse0
- Direction
- not necessarye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The complex medium consisting of minerals, trace elements, vitamins, sucrose and growth substances is not necessary for the initiation and the early growth of pollen callus, but all these factors in the medium are beneficial for the further deve- lopment of the callus.”
t0“ON THE CONDITIONS FOR THE INDUCTION OF RICE POLLEN PLANTLETS AND CERTAIN FACTORS AFFECTING THE FREQUENCY OF INDUCTION”
Claim · model-written summary
The factors in complex medium benefited further callus development.
- Taxon
- RICEOryza sativat0
- Treatment
- minerals, trace elements, vitamins, sucrose and growth substancese0
- Outcome
- further deve- lopment of the calluse0
- Direction
- beneficiale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The complex medium consisting of minerals, trace elements, vitamins, sucrose and growth substances is not necessary for the initiation and the early growth of pollen callus, but all these factors in the medium are beneficial for the further deve- lopment of the callus.”
t0“ON THE CONDITIONS FOR THE INDUCTION OF RICE POLLEN PLANTLETS AND CERTAIN FACTORS AFFECTING THE FREQUENCY OF INDUCTION”
Claim · model-written summary
Sucrose regulated medium osmotic pressure.
- Taxon
- RICEOryza sativat0
- Treatment
- Sucrosee0
- Outcome
- osmotic pressuree0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose in the medium plays a significant role in the regulation of osmotic pressure. Excessively high concentration of sucrose inhibits callus formation.”
t0“ON THE CONDITIONS FOR THE INDUCTION OF RICE POLLEN PLANTLETS AND CERTAIN FACTORS AFFECTING THE FREQUENCY OF INDUCTION”
Claim · model-written summary
Excessively high sucrose inhibited callus formation.
- Taxon
- RICEOryza sativat0
- Treatment
- Excessively high concentration of sucrosee0
- Outcome
- callus formatione0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose in the medium plays a significant role in the regulation of osmotic pressure. Excessively high concentration of sucrose inhibits callus formation.”
t0“ON THE CONDITIONS FOR THE INDUCTION OF RICE POLLEN PLANTLETS AND CERTAIN FACTORS AFFECTING THE FREQUENCY OF INDUCTION”
Claim · model-written summary
Coconut milk, casein hydrolysate and mononucleotide benefited callus formation.
- Taxon
- RICEOryza sativat0
- Treatment
- Coconut milk, casein hydrolysate, mononucleotidee0
- Outcome
- callus formatione0
- Direction
- beneficiale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Coconut milk, casein hydrolysate, mononucleotide and some other organic sup- plements are obviously beneficial to callus formation and the subsequent differentia- tion of the organs.”
t0“ON THE CONDITIONS FOR THE INDUCTION OF RICE POLLEN PLANTLETS AND CERTAIN FACTORS AFFECTING THE FREQUENCY OF INDUCTION”
Claim · model-written summary
The organic supplements benefited subsequent organ differentiation.
- Taxon
- RICEOryza sativat0
- Treatment
- Coconut milk, casein hydrolysate, mononucleotidee0
- Outcome
- subsequent differentia- tion of the organse0
- Direction
- beneficiale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Coconut milk, casein hydrolysate, mononucleotide and some other organic sup- plements are obviously beneficial to callus formation and the subsequent differentia- tion of the organs.”
t0“ON THE CONDITIONS FOR THE INDUCTION OF RICE POLLEN PLANTLETS AND CERTAIN FACTORS AFFECTING THE FREQUENCY OF INDUCTION”
Claim · model-written summary
2-chlorethvl-phosphonic acid supplementation greatly promoted pollen callus frequency.
- Taxon
- riceOryza sativae0
- Treatment
- 2-chlorethvl-phosphonic acid (8 ppm, 40 ppm)e0
- Outcome
- frequency of the formation of the pollen calluse0
- Direction
- greatly promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“If the culture medium is supplemented with 2-chlorethvl-phosphonic acid (8 ppm, 40 ppm) or when the raceme of the rice is pretreated at 10℃, for 48 hours, the frequency of the formation of the pollen callus will be greatly promoted.”
Claim · model-written summary
Pretreating the rice raceme at 10℃ for 48 hours greatly promoted pollen callus frequency.
- Taxon
- riceOryza sativae0
- Treatment
- pretreated at 10℃, for 48 hourse0
- Outcome
- frequency of the formation of the pollen calluse0
- Direction
- greatly promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“If the culture medium is supplemented with 2-chlorethvl-phosphonic acid (8 ppm, 40 ppm) or when the raceme of the rice is pretreated at 10℃, for 48 hours, the frequency of the formation of the pollen callus will be greatly promoted.”
Claim · model-written summary
Older pollen callus gave lower plantlet induction frequency.
- Taxon
- RICEOryza sativat0
- Organ / stage
- pollen calluse0
- Outcome
- induction frequency of the pollen plantletse0
- Direction
- lowere0
- Qualifier
- older the callus ise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The age of the pollen callus affects the ability of the organ differentiation; the older the callus is, the lower the induction frequency of the pollen plantlets.”
t0“ON THE CONDITIONS FOR THE INDUCTION OF RICE POLLEN PLANTLETS AND CERTAIN FACTORS AFFECTING THE FREQUENCY OF INDUCTION”
The same questions in other papers
micronutrient · growth · Oryza sativa (rice)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
sucrose · growth · Oryza sativa (rice)Results agree
30 papers in the same direction as this paper
- positive
- positive
- positive
27 papers more in this group
See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.