Abstract
The basic leucine zipper (bZIP) is a plant-specific transcription factor family that plays crucial roles in response to biotic and abiotic stresses. However, little is known about the function of bZIP genes in soybean. In this study, we isolated a bZIP gene, GmbZIP19, from soybean. A subcellular localization study of GmbZIP19 revealed its nucleus localization. We showed that GmbZIP19 expression was significantly induced by ABA (abscisic acid), JA (jasmonic acid) and SA (salicylic acid), but reduced under salt and drought stress conditions. Further, GmbZIP19 overexpression Arabidopsis lines showed increased resistance to S. sclerotiorum and Pseudomonas syringae associated with upregulated ABA-, JA-, ETH- (ethephon-)and SA-induced marker genes expression, but exhibited sensitivity to salt and drought stresses in association with destroyed stomatal closure and downregulated the salt and drought stresses marker genes’ expression. We generated a soybean transient GmbZIP19 overexpression line, performed a Chromatin immunoprecipitation assay and found that GmbZIP19 bound to promoters of ABA-, JA-, ETH-, and SA-induced marker genes in soybean. The yeast one-hybrid verified the combination. The current study suggested that GmbZIP19 is a positive regulator of pathogen resistance and a negative regulator of salt and drought stress tolerance.
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Claims 13
Claim · model-written summary
GmbZIP19 was isolated from soybean.
- Taxon
- soybean
Glycine maxe0 - Gene
- GmbZIP19e0
- Outcome
- isolated a bZIP genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated a bZIP gene, GmbZIP19, from soybean”
Claim · model-written summary
GmbZIP19 localized to the nucleus.
- Taxon
- soybeanGlycine maxe1
- Gene
- GmbZIP19e0
- Outcome
- nucleus localizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmbZIP19 revealed its nucleus localization”
e1“we isolated a bZIP gene, GmbZIP19, from soybean”
Claim · model-written summary
ABA induced GmbZIP19 expression.
- Taxon
- soybeanGlycine maxe1
- Treatment
- ABA (abscisic acid)e0
- Gene
- GmbZIP19e0
- Outcome
- GmbZIP19 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmbZIP19 expression was significantly induced by ABA (abscisic acid), JA (jasmonic acid) and SA (salicylic acid)”
e1“we isolated a bZIP gene, GmbZIP19, from soybean”
Claim · model-written summary
JA induced GmbZIP19 expression.
- Taxon
- soybeanGlycine maxe1
- Treatment
- JA (jasmonic acid)e0
- Gene
- GmbZIP19e0
- Outcome
- GmbZIP19 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmbZIP19 expression was significantly induced by ABA (abscisic acid), JA (jasmonic acid) and SA (salicylic acid)”
e1“we isolated a bZIP gene, GmbZIP19, from soybean”
Claim · model-written summary
SA induced GmbZIP19 expression.
- Taxon
- soybeanGlycine maxe1
- Treatment
- SA (salicylic acid)e0
- Gene
- GmbZIP19e0
- Outcome
- GmbZIP19 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmbZIP19 expression was significantly induced by ABA (abscisic acid), JA (jasmonic acid) and SA (salicylic acid)”
e1“we isolated a bZIP gene, GmbZIP19, from soybean”
Claim · model-written summary
GmbZIP19 overexpression increased Arabidopsis resistance to S. sclerotiorum.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- GmbZIP19 overexpressione0
- Interactor
- S. sclerotiorume0pathogen
- Gene
- GmbZIP19e0
- Outcome
- resistance to S. sclerotiorume0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmbZIP19 overexpression Arabidopsis lines showed increased resistance to S. sclerotiorum and Pseudomonas syringae”
Claim · model-written summary
GmbZIP19 overexpression increased Arabidopsis resistance to Pseudomonas syringae.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- GmbZIP19 overexpressione0
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee0pathogen - Gene
- GmbZIP19e0
- Outcome
- Pseudomonas syringaee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmbZIP19 overexpression Arabidopsis lines showed increased resistance to S. sclerotiorum and Pseudomonas syringae”
Claim · model-written summary
Overexpression upregulated hormone-induced marker gene expression.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- GmbZIP19 overexpressione1
- Outcome
- ABA-, JA-, ETH- (ethephon-)and SA-induced marker genes expressione0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“associated with upregulated ABA-, JA-, ETH- (ethephon-)and SA-induced marker genes expression”
e1“GmbZIP19 overexpression Arabidopsis lines”
Claim · model-written summary
Overexpressing Arabidopsis lines were sensitive to salt stress.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- GmbZIP19 overexpressione1
- Outcome
- sensitivity to salte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but exhibited sensitivity to salt and drought stresses”
e1“GmbZIP19 overexpression Arabidopsis lines”
Claim · model-written summary
Overexpressing Arabidopsis lines were sensitive to drought stress.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- GmbZIP19 overexpressione1
- Outcome
- sensitivity to salt and drought stressese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but exhibited sensitivity to salt and drought stresses”
e1“GmbZIP19 overexpression Arabidopsis lines”
Claim · model-written summary
Overexpression was associated with disrupted stomatal closure.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- GmbZIP19 overexpressione1
- Outcome
- stomatal closuree0
- Direction
- destroyede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in association with destroyed stomatal closure and downregulated the salt and drought stresses marker genes’ expression”
e1“GmbZIP19 overexpression Arabidopsis lines”
Claim · model-written summary
GmbZIP19 bound promoters of hormone-induced marker genes in soybean.
- Taxon
- soybeanGlycine maxe0
- Gene
- GmbZIP19e0
- Outcome
- bound to promoters of ABA-, JA-, ETH-, and SA-induced marker genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmbZIP19 bound to promoters of ABA-, JA-, ETH-, and SA-induced marker genes in soybean”
Claim · model-written summary
Authors interpret GmbZIP19 as a positive regulator of pathogen resistance and negative regulator of abiotic stress tolerance.
- Taxon
- soybeanGlycine maxe1
- Gene
- GmbZIP19e0
- Outcome
- pathogen resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmbZIP19 is a positive regulator of pathogen resistance and a negative regulator of salt and drought stress tolerance”
e1“we isolated a bZIP gene, GmbZIP19, from soybean”
The same questions in other papers
salicylic acid · molecular: expression · Glycine max (soybean)Positive and negative results
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- no effect
- negative
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See all 11 papers →(+)-abscisic acid · molecular: expression · Glycine max (soybean)Positive and negative results
17 papers in the same direction as this paper · 8 in the opposite direction · 1 reporting no difference from an alternative
- positive
- no effect
- no effect
23 papers more in this group
See all 27 papers →jasmonic acid · molecular: expression · Glycine max (soybean)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.