Abstract
This paper reports on the identification and characterization of a new ATP-binding cassette (ABC) transporter which was identified as a salicylic acid-induced gene from soybean (Glycine max cv. Williams 82) in a subtractive suppression hybridization approach. A fragment of an ABC-transporter gene was used to isolate a full-length cDNA clone for this gene with a length of 4750 bp. The encoded protein has a length of 1447 amino acids and is composed of two similar repeat units typical of full-size ABC transporters. The sequence displays a close relationship to plant pleiotropic drug resistance (PDR)-type transporters and, on a homology basis, clusters together with the Arabidopsis thaliana PDR12 gene, suggesting GmPDR12 as a name for the gene isolated from soybean. GmPDR12 is rapidly responsive to salicylic acid and methyl jasmonate. The mRNA starts to accumulate 30 min after the addition of the signalling compounds. Salicylic acid is required for the execution of the hypersensitive reaction in soybean cell suspension cultures inoculated with Pseudomonas syringae pv. glycinea. It has been demonstrated previously that salicylic acid can be substituted by a variety of functional analogues of salicylic acid. All of these compounds lead to a strong and rapid transcriptional activation of GmPDR12, suggesting a shared signalling pathway.
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Claims 7
Claim · model-written summary
A full-length soybean ABC transporter cDNA clone was isolated with a length of 4750 bp.
- Taxon
- soybean
Glycine maxt0 - Gene
- ABC-transporter genee0
- Outcome
- full-length cDNA clonee0
- Value
- 4750 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A fragment of an ABC-transporter gene was used to isolate a full-length cDNA clone for this gene with a length of 4750 bp”
t0“from soybean”
Claim · model-written summary
The encoded ABC transporter is 1447 amino acids long and has two similar repeat units.
- Taxon
- soybeanGlycine maxt0
- Gene
- encoded proteine0
- Outcome
- lengthe0
- Value
- 1447 amino acidse0
- Qualifier
- two similar repeat unitse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The encoded protein has a length of 1447 amino acids and is composed of two similar repeat units typical of full-size ABC transporters”
t0“from soybean”
Claim · model-written summary
The soybean sequence clustered with Arabidopsis PDR12 and was proposed to be named GmPDR12.
- Taxon
- soybeanGlycine maxe0
- Gene
- GmPDR12e0
- Outcome
- clusters together with the Arabidopsis thaliana PDR12 genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The sequence displays a close relationship to plant pleiotropic drug resistance (PDR)-type transporters and, on a homology basis, clusters together with the Arabidopsis thaliana PDR12 gene, suggesting GmPDR12 as a name for the gene isolated from soybean”
Claim · model-written summary
GmPDR12 mRNA accumulated within 30 minutes after salicylic acid application.
- Taxon
- soybeanGlycine maxt0
- Treatment
- salicylic acide0
- Gene
- GmPDR12e0
- Outcome
- mRNAe0
- Direction
- accumulatee0
- Value
- 30 mine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmPDR12 is rapidly responsive to salicylic acid and methyl jasmonate. The mRNA starts to accumulate 30 min after the addition of the signalling compounds”
t0“from soybean”
Claim · model-written summary
GmPDR12 mRNA accumulated within 30 minutes after methyl jasmonate application.
- Taxon
- soybeanGlycine maxt0
- Treatment
- methyl jasmonatee0
- Gene
- GmPDR12e0
- Outcome
- mRNAe0
- Direction
- accumulatee0
- Value
- 30 mine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmPDR12 is rapidly responsive to salicylic acid and methyl jasmonate. The mRNA starts to accumulate 30 min after the addition of the signalling compounds”
t0“from soybean”
Claim · model-written summary
Salicylic acid functional analogues strongly and rapidly activated GmPDR12 transcription.
- Taxon
- soybeanGlycine maxt0
- Treatment
- functional analogues of salicylic acide1
- Gene
- GmPDR12e0
- Outcome
- transcriptional activatione0
- Direction
- strong and rapide0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All of these compounds lead to a strong and rapid transcriptional activation of GmPDR12”
e1“functional analogues of salicylic acid”
t0“from soybean”
Claim · model-written summary
The authors infer a shared signaling pathway from the similar activation by salicylic acid analogues.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmPDR12e0
- Outcome
- shared signalling pathwaye0
- Qualifier
- suggestinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All of these compounds lead to a strong and rapid transcriptional activation of GmPDR12, suggesting a shared signalling pathway”
t0“from soybean”
The same questions in other papers
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- no effect
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See all 10 papers →salicylic acid · molecular: expression · Glycine max (soybean)Positive and negative results
8 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.