Abstract
Soybean cyst nematode (SCN, Heterodera glycines), one of the most devastating soybean pathogens, causes a significant yield loss in soybean production. One of the most effective ways to manage SCN is to grow resistant cultivars. Therefore, comparative study using resistant and susceptible soybean cultivars provides a powerful tool to identify new genes involved in soybean SCN resistance. In the present study, a transcriptome analysis was carried out using both the resistant (PI88788) and susceptible (Williams 82) soybean cultivars to characterize the responses to nematode infection. Various defense-related genes and different pathways involved in nematode resistance were recognized as being highly expressed in resistant cultivar. Promoter-GUS analysis was conducted to monitor the spatial expression pattern of the genes highly induced by nematode infection. Two nematode-inducible promoters for Glyma.05g147000 (encoding caffeoyl-CoA O-methyltransferase) and Glyma.06g036700 (encoding cupredoxin superfamily protein) were characterized, and the promoters could efficiently drive the expression of known nematode resistance genes (α-SNAP Rhg1 HC or GmSHMT) to affect soybean SCN resistance. Interestingly, expression of the cupredoxin family genes was upregulated not only by SCN, but also by jasmonic acid treatment. DNA sequence analysis identified that a conserved motif (GGTGCATG) with high similarity to SCNbox1 and GC-rich element is enriched in their promoter regions, suggesting its potential to serve as a nematode-responsive regulatory element. Overexpression of Glyma.06g036700 significantly enhanced soybean resistance to cyst nematode. Overall, our findings not only highlight the essential role of cupredoxin family genes in SCN resistance, but also offer potential functional tools to develop nematode resistance in crops.
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Claims 9
Claim · model-written summary
Defense-related genes were highly expressed in the resistant soybean cultivar compared with the susceptible cultivar during nematode infection.
- Taxon
- soybean
Glycine maxe1 - Material
- resistant (PI88788)e1
- Interactor
- Soybean cyst nematode
Heterodera glycinese0pathogen - Outcome
- defense-related genese2
- Direction
- highly expressede2
- Comparator
- susceptible (Williams 82)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean cyst nematode (SCN, Heterodera glycines), one of the most devastating soybean pathogens”
e1“a transcriptome analysis was carried out using both the resistant (PI88788) and susceptible (Williams 82) soybean cultivars”
e2“Various defense-related genes and different pathways involved in nematode resistance were recognized as being highly expressed in resistant cultivar”
Claim · model-written summary
The promoter of Glyma.05g147000 is nematode-inducible.
- Taxon
- soybeanGlycine maxe1
- Gene
- Glyma.05g147000e3
- Outcome
- nematode-inducible promoterse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a transcriptome analysis was carried out using both the resistant (PI88788) and susceptible (Williams 82) soybean cultivars”
e3“Two nematode-inducible promoters for Glyma.05g147000 (encoding caffeoyl-CoA O-methyltransferase) and Glyma.06g036700 (encoding cupredoxin superfamily protein) were characterized”
Claim · model-written summary
The promoter of Glyma.06g036700 is nematode-inducible.
- Taxon
- soybeanGlycine maxe1
- Gene
- Glyma.06g036700e3
- Outcome
- nematode-inducible promoterse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a transcriptome analysis was carried out using both the resistant (PI88788) and susceptible (Williams 82) soybean cultivars”
e3“Two nematode-inducible promoters for Glyma.05g147000 (encoding caffeoyl-CoA O-methyltransferase) and Glyma.06g036700 (encoding cupredoxin superfamily protein) were characterized”
Claim · model-written summary
The two promoters could drive expression of known nematode resistance genes, affecting soybean SCN resistance.
- Taxon
- soybeanGlycine maxe1
- Interactor
- SCNe4pathogen
- Gene
- α-SNAP Rhg1 HC or GmSHMTe4
- Outcome
- expression of known nematode resistance genese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a transcriptome analysis was carried out using both the resistant (PI88788) and susceptible (Williams 82) soybean cultivars”
e4“the promoters could efficiently drive the expression of known nematode resistance genes (α-SNAP Rhg1 HC or GmSHMT) to affect soybean SCN resistance”
Claim · model-written summary
Nematode infection upregulated expression of the cupredoxin family genes.
- Taxon
- soybeanGlycine maxe1
- Treatment
- SCNe5
- Interactor
- SCNe5pathogen
- Outcome
- expression of the cupredoxin family genese5
- Direction
- upregulatede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a transcriptome analysis was carried out using both the resistant (PI88788) and susceptible (Williams 82) soybean cultivars”
e5“expression of the cupredoxin family genes was upregulated not only by SCN, but also by jasmonic acid treatment”
Claim · model-written summary
Jasmonic acid treatment upregulated expression of the cupredoxin family genes.
- Taxon
- soybeanGlycine maxe1
- Treatment
- jasmonic acid treatmente5
- Outcome
- expression of the cupredoxin family genese5
- Direction
- upregulatede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a transcriptome analysis was carried out using both the resistant (PI88788) and susceptible (Williams 82) soybean cultivars”
e5“expression of the cupredoxin family genes was upregulated not only by SCN, but also by jasmonic acid treatment”
Claim · model-written summary
A conserved motif (GGTGCATG) is enriched in the promoter regions of the cupredoxin genes.
- Taxon
- soybeanGlycine maxe1
- Outcome
- enriched in their promoter regionse6
- Qualifier
- GGTGCATGe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a transcriptome analysis was carried out using both the resistant (PI88788) and susceptible (Williams 82) soybean cultivars”
e6“DNA sequence analysis identified that a conserved motif (GGTGCATG) with high similarity to SCNbox1 and GC-rich element is enriched in their promoter regions”
Claim · model-written summary
Overexpression of Glyma.06g036700 significantly enhanced soybean resistance to cyst nematode.
- Taxon
- soybeanGlycine maxe7
- Treatment
- Overexpression of Glyma.06g036700e7
- Interactor
- cyst nematodee7pathogen
- Gene
- Glyma.06g036700e7
- Outcome
- resistance to cyst nematodee7
- Direction
- enhancede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Overexpression of Glyma.06g036700 significantly enhanced soybean resistance to cyst nematode”
Claim · model-written summary
Cupredoxin family genes play an essential role in soybean SCN resistance.
- Taxon
- soybeanGlycine maxe1
- Interactor
- SCNe2pathogen
- Gene
- cupredoxin family genese2
- Outcome
- SCN resistancee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a transcriptome analysis was carried out using both the resistant (PI88788) and susceptible (Williams 82) soybean cultivars”
e2“our findings not only highlight the essential role of cupredoxin family genes in SCN resistance”
The same questions in other papers
jasmonic acid · molecular: expression · Glycine max (soybean)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.