Abstract
Degenerate oligonucleotide primers were designed according to the Arabidopsis RPS2 and tobacco N and flax L6, and two open-reading NBS resistance gene analogs were finally characterized, which were named as RNEAU-1 and RNEAU-2 respectively from the RNA of soybean cultivar Suinong 10, resistance to phytophthora sojae. The nucleotide sequence of each analog was both 513bp in length that code 171 amino acids. Gene specific primers were designed according to the sequence of RNEAU-1, and a full length soybean resistance-related gene SR1 was obtained through the 5'RACE and 3'RACE methods. The nucleotide sequence of SR1 was 3574bp in length, which included a 3411-bp complete open reading frame encoding SR1 protein of 1137 amino acids, a 72-bp 5'non-translated region (5'NTR), a 68-bp3'non-translated region (3'NTR) and 20-bp poly (A) tails. The deduced 1137-amino acids SR1 protein consisted of Toll/Interleukin receptor (TIR) domain, nucleotide binding site (NBS) domain, leucine-rich repeats (LRR) domain, hydrophobic domain and conserved domain2, which were the conserved domains of plant resistance genes. The SR1 gene and the SR1-related sequences were presented as 2--4 members in soybean genome according to the result of southern blotting. The SR1 gene was a consitutive gene with low abundance in soybean genome. The transcripts of SR1 gene were detected in roots, stem and leaves of soybean. The gene appeared not to be induced by phytophthora sojae inoculation and salicylic acid treatment. Due to these features SR1 gene was a member of class I TIR-NBS-LRR type resistance gene. The gene has been submitted to the GenBank database, and the accession number is AY193892. A PCR reaction was done with the genome DNA of Suinong10 as template and a 3972 bp transcription nucleotide sequence was acquired which consisted of 5 exons and 4 introns.
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Claims 12
Claim · model-written summary
Two soybean NBS resistance gene analogs, RNEAU-1 and RNEAU-2, were characterized.
- Taxon
- soybean
Glycine maxe0 - Material
- Suinong 10e0
- Gene
- RNEAU-1 and RNEAU-2e0
- Outcome
- two open-reading NBS resistance gene analogse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two open-reading NBS resistance gene analogs were finally characterized, which were named as RNEAU-1 and RNEAU-2 respectively from the RNA of soybean cultivar Suinong 10”
Claim · model-written summary
Each analog was 513 bp long and encoded 171 amino acids.
- Taxon
- soybeanGlycine maxt0
- Outcome
- nucleotide sequence of each analoge0
- Value
- 513bpe0
- Qualifier
- 171 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The nucleotide sequence of each analog was both 513bp in length that code 171 amino acids.”
t0“soybean”
Claim · model-written summary
A full-length soybean resistance-related gene SR1 was obtained.
- Taxon
- soybeanGlycine maxe0
- Gene
- SR1e0
- Outcome
- full length soybean resistance-related gene SR1e0
- Value
- full lengthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a full length soybean resistance-related gene SR1 was obtained through the 5'RACE and 3'RACE methods”
Claim · model-written summary
SR1 was 3574 bp long, with a 3411-bp open reading frame encoding a 1137-amino-acid protein and untranslated regions and a poly(A) tail.
- Taxon
- soybeanGlycine maxt0
- Gene
- SR1e0
- Outcome
- nucleotide sequence of SR1e0
- Value
- 3574bpe0
- Qualifier
- 3411-bp complete open reading framee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The nucleotide sequence of SR1 was 3574bp in length, which included a 3411-bp complete open reading frame encoding SR1 protein of 1137 amino acids, a 72-bp 5'non-translated region (5'NTR), a 68-bp3'non-translated region (3'NTR) and 20-bp poly (A) tails.”
t0“soybean”
Claim · model-written summary
SR1 protein contained TIR, NBS, LRR, hydrophobic, and conserved domain2 regions.
- Taxon
- soybeanGlycine maxt0
- Gene
- SR1 proteine0
- Outcome
- Toll/Interleukin receptor (TIR) domain, nucleotide binding site (NBS) domain, leucine-rich repeats (LRR) domain, hydrophobic domain and conserved domain2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deduced 1137-amino acids SR1 protein consisted of Toll/Interleukin receptor (TIR) domain, nucleotide binding site (NBS) domain, leucine-rich repeats (LRR) domain, hydrophobic domain and conserved domain2”
t0“soybean”
Claim · model-written summary
SR1 and related sequences occurred as 2--4 members in the soybean genome.
- Taxon
- soybeanGlycine maxe0
- Gene
- SR1 genee0
- Outcome
- SR1-related sequencese0
- Value
- 2--4 memberse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The SR1 gene and the SR1-related sequences were presented as 2--4 members in soybean genome according to the result of southern blotting.”
Claim · model-written summary
SR1 was constitutively expressed at low abundance.
- Taxon
- soybeanGlycine maxe0
- Gene
- SR1 genee0
- Outcome
- consitutive gene with low abundancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The SR1 gene was a consitutive gene with low abundance in soybean genome.”
Claim · model-written summary
SR1 transcripts were detected in soybean roots, stems, and leaves.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- roots, stem and leavese0
- Gene
- SR1 genee0
- Outcome
- transcripts of SR1 gene were detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcripts of SR1 gene were detected in roots, stem and leaves of soybean.”
Claim · model-written summary
Phytophthora sojae inoculation did not appear to induce SR1.
- Taxon
- soybeanGlycine maxt0
- Treatment
- phytophthora sojae inoculatione0
- Interactor
- phytophthora sojaePhytophthora sojaee0pathogen
- Gene
- genee0
- Outcome
- gene appeared not to be inducede0
- Direction
- not to be inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gene appeared not to be induced by phytophthora sojae inoculation”
t0“soybean”
Claim · model-written summary
Salicylic acid treatment did not appear to induce SR1.
- Taxon
- soybeanGlycine maxt0
- Treatment
- salicylic acid treatmente0
- Outcome
- gene appeared not to be inducede0
- Direction
- not to be inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gene appeared not to be induced by phytophthora sojae inoculation and salicylic acid treatment.”
t0“soybean”
Claim · model-written summary
SR1 was classified as a class I TIR-NBS-LRR resistance gene.
- Taxon
- soybeanGlycine maxt0
- Gene
- SR1 genee0
- Outcome
- class I TIR-NBS-LRR type resistance genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SR1 gene was a member of class I TIR-NBS-LRR type resistance gene.”
t0“soybean”
Claim · model-written summary
A genomic SR1 sequence of 3972 bp with five exons and four introns was obtained from Suinong10.
- Taxon
- soybeanGlycine maxt0
- Material
- Suinong10e0
- Outcome
- transcription nucleotide sequencee0
- Value
- 3972 bpe0
- Qualifier
- 5 exons and 4 intronse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the genome DNA of Suinong10 as template and a 3972 bp transcription nucleotide sequence was acquired which consisted of 5 exons and 4 introns.”
t0“soybean”
The same questions in other papers
salicylic acid · molecular: expression · Glycine max (soybean)Positive and negative results
1 paper in the same direction as this paper · 9 in the opposite direction
- positive
- positive
- positive
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See all 11 papers →inoculation · Phytophthora sojae · Glycine max (soybean)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.