Abstract
Drought is a major abiotic stress that limits wheat production worldwide. To ensure food security for the rapidly increasing world population, improving wheat yield under drought stress is urgent and relevant. In this study, an RNA-seq analysis was conducted to study the effect of drought on wheat transcriptome changes during reproductive stages under field conditions. Our results indicated that drought stress during early reproductive periods had a more severe impact on wheat development, gene expression and yield than drought stress during flowering. In total, 115,656 wheat genes were detected, including 309 differentially expressed genes (DEGs) which responded to drought at various developmental stages. These DEGs were involved in many critical processes including floral development, photosynthetic activity and stomatal movement. At early developmental stages, the proteins of drought-responsive DEGs were mainly located in the nucleus, peroxisome, mitochondria, plasma membrane and chloroplast, indicating that these organelles play critical roles in drought tolerance in wheat. Furthermore, the validation of five DEGs confirmed their responsiveness to drought under different genetic backgrounds. Functional verification of DEGs of interest will occur in our subsequent research. Collectively, the results of this study not only advanced our understanding of wheat transcriptome changes under drought stress during early reproductive stages but also provided useful targets to manipulate drought tolerance in wheat at different development stages.
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Claims 8
Claim · model-written summary
Early reproductive drought had a more severe effect on wheat development than drought during flowering.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- drought stress during early reproductive periodse0
- Outcome
- wheat developmente0
- Direction
- more severe impacte0
- Comparator
- drought stress during floweringe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“drought stress during early reproductive periods had a more severe impact on wheat development, gene expression and yield than drought stress during flowering.”
Claim · model-written summary
Early reproductive drought had a more severe effect on wheat gene expression than drought during flowering.
- Taxon
- wheatTriticum aestivume0
- Treatment
- drought stress during early reproductive periodse0
- Outcome
- gene expressione0
- Direction
- more severe impacte0
- Comparator
- drought stress during floweringe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“drought stress during early reproductive periods had a more severe impact on wheat development, gene expression and yield than drought stress during flowering.”
Claim · model-written summary
Early reproductive drought had a more severe effect on wheat yield than drought during flowering.
- Taxon
- wheatTriticum aestivume0
- Treatment
- drought stress during early reproductive periodse0
- Outcome
- yielde0
- Direction
- more severe impacte0
- Comparator
- drought stress during floweringe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“drought stress during early reproductive periods had a more severe impact on wheat development, gene expression and yield than drought stress during flowering.”
Claim · model-written summary
RNA sequencing detected 115,656 wheat genes.
- Taxon
- wheatTriticum aestivume0
- Outcome
- genes were detectede0
- Value
- 115,656e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In total, 115,656 wheat genes were detected”
Claim · model-written summary
The analysis identified 309 genes differentially expressed in response to drought across developmental stages.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- droughte0
- Outcome
- differentially expressed genes (DEGs)e0
- Value
- 309e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“309 differentially expressed genes (DEGs) which responded to drought at various developmental stages.”
t0“Wheat”
Claim · model-written summary
Drought responsive DEGs were involved in floral development, photosynthetic activity, and stomatal movement.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- floral development, photosynthetic activity and stomatal movemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These DEGs were involved in many critical processes including floral development, photosynthetic activity and stomatal movement.”
t0“Wheat”
Claim · model-written summary
At early developmental stages, proteins of drought responsive DEGs were mainly located in five named organelles.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- early developmental stagese0
- Outcome
- mainly located in the nucleus, peroxisome, mitochondria, plasma membrane and chloroplaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At early developmental stages, the proteins of drought-responsive DEGs were mainly located in the nucleus, peroxisome, mitochondria, plasma membrane and chloroplast”
t0“Wheat”
Claim · model-written summary
Validation of five DEGs confirmed drought responsiveness across genetic backgrounds.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- responsiveness to droughte0
- Value
- five DEGse0
- Qualifier
- under different genetic backgroundse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the validation of five DEGs confirmed their responsiveness to drought under different genetic backgrounds.”
t0“Wheat”
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