Abstract
Cucumber is an important horticultural vegetable crop with considerable potential for proanthocyanidin extraction. Flavonoids play crucial roles in plant defense and growth regulation, with MYB transcription factors and miRNAs being key regulators of their biosynthesis. In this study, we identified a differentially expressed miRNA , Cs-miR858 , in cucumber cultivars with contrasting flavonoid contents and root rot resistance. Cs-miR858 was found to negatively regulate the accumulation of Proanthocyanidin (PA) by targeting CsMYB60 , a key regulator of flavonoid biosynthesis. Overexpression of Cs-miR858 in cucumber via transient and stable transformation reduced PA content by downregulating structural genes such as CsLAR and increased susceptibility to Fusarium solani ( F. solani) . Conversely, silencing Cs-miR858 promoted PA biosynthesis and enhanced resistance. Molecular validation demonstrated that Cs-miR858 directly targets the coding sequence of CsMYB60 . Additionally, heterologous expression of Cs-miR858 in Arabidopsis inhibited anthocyanin and PA accumulation by targeting CsMYB60 . Furthermore, we found that CsBBX22 bound to the Cs-miR858 promoter and induced its expression, reducing PA accumulation in cucumber. Our findings unveil a miRNA-MYB regulatory module in which Cs-miR858 fine-tunes flavonoid metabolism by suppressing CsMYB60 , thereby modulating cucumber's resistance to F. solani . This study provides a theoretical basis for breeding new cucumber cultivars with high flavonoid content, strong disease resistance, and pronounced medicinal value.
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Claims 10
Claim · model-written summary
Cs-miR858 was differentially expressed in cucumber cultivars with contrasting flavonoid contents and root rot resistance.
- Taxon
- cucumber
Cucumis sativuse1 - Gene
- Cs-miR858e1
- Outcome
- differentially expressed miRNAe1
- Qualifier
- contrasting flavonoid contents and root rot resistancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we identified a differentially expressed miRNA , Cs-miR858 , in cucumber cultivars with contrasting flavonoid contents and root rot resistance”
Claim · model-written summary
Cs-miR858 negatively regulates proanthocyanidin accumulation by targeting CsMYB60.
- Taxon
- cucumberCucumis sativust0
- Gene
- Cs-miR858e2
- Outcome
- accumulation of Proanthocyanidin (PA)e2
- Qualifier
- by targeting CsMYB60e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Fusarium solani in cucumber”
e2“Cs-miR858 was found to negatively regulate the accumulation of Proanthocyanidin (PA) by targeting CsMYB60”
Claim · model-written summary
Overexpression of Cs-miR858 in cucumber reduced proanthocyanidin content by downregulating CsLAR.
- Taxon
- cucumberCucumis sativuse3
- Treatment
- Overexpression of Cs-miR858e3
- Gene
- CsLARe3
- Outcome
- PA contente3
- Direction
- reducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Overexpression of Cs-miR858 in cucumber via transient and stable transformation reduced PA content by downregulating structural genes such as CsLAR and increased susceptibility to Fusarium solani ( F. solani) .”
Claim · model-written summary
Overexpression of Cs-miR858 in cucumber increased susceptibility to Fusarium solani.
- Taxon
- cucumberCucumis sativuse3
- Treatment
- Overexpression of Cs-miR858e3
- Interactor
- Fusarium solani
_Sacc._5393379.jpg?width=60)
Fusarium solanie3pathogen - Outcome
- susceptibility to Fusarium solanie3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Sacc._5393379.jpg?width=500)
Verbatim
e3“Overexpression of Cs-miR858 in cucumber via transient and stable transformation reduced PA content by downregulating structural genes such as CsLAR and increased susceptibility to Fusarium solani ( F. solani) .”
Claim · model-written summary
Silencing Cs-miR858 promoted proanthocyanidin biosynthesis in cucumber.
- Taxon
- cucumberCucumis sativust0
- Treatment
- silencing Cs-miR858e4
- Outcome
- PA biosynthesise4
- Direction
- promotede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Fusarium solani in cucumber”
e4“Conversely, silencing Cs-miR858 promoted PA biosynthesis and enhanced resistance”
Claim · model-written summary
Silencing Cs-miR858 enhanced resistance to Fusarium solani in cucumber.
- Taxon
- cucumberCucumis sativust0
- Treatment
- silencing Cs-miR858e4
- Interactor
- Fusarium solaniFusarium solanie3pathogen
- Outcome
- resistancee4
- Direction
- enhancede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Fusarium solani in cucumber”
e3“Overexpression of Cs-miR858 in cucumber via transient and stable transformation reduced PA content by downregulating structural genes such as CsLAR and increased susceptibility to Fusarium solani ( F. solani) .”
e4“Conversely, silencing Cs-miR858 promoted PA biosynthesis and enhanced resistance”
Claim · model-written summary
Cs-miR858 directly targets the coding sequence of CsMYB60.
- Taxon
- cucumberCucumis sativust0
- Gene
- CsMYB60e5
- Outcome
- coding sequence of CsMYB60e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Fusarium solani in cucumber”
e5“Molecular validation demonstrated that Cs-miR858 directly targets the coding sequence of CsMYB60”
Claim · model-written summary
Heterologous Cs-miR858 expression in Arabidopsis inhibited anthocyanin and proanthocyanidin accumulation by targeting CsMYB60.
- Taxon
- Arabidopsis
Arabidopsise6 - Treatment
- heterologous expression of Cs-miR858e6
- Gene
- CsMYB60e6
- Outcome
- anthocyanin and PA accumulatione6
- Direction
- inhibitede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“heterologous expression of Cs-miR858 in Arabidopsis inhibited anthocyanin and PA accumulation by targeting CsMYB60”
Claim · model-written summary
CsBBX22 bound the Cs-miR858 promoter and induced its expression in cucumber.
- Taxon
- cucumberCucumis sativuse7
- Gene
- CsBBX22e7
- Outcome
- induced its expressione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“CsBBX22 bound to the Cs-miR858 promoter and induced its expression, reducing PA accumulation in cucumber”
Claim · model-written summary
CsBBX22-induced Cs-miR858 expression reduces proanthocyanidin accumulation in cucumber.
- Taxon
- cucumberCucumis sativuse7
- Gene
- CsBBX22e7
- Outcome
- PA accumulatione7
- Direction
- reducinge7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“CsBBX22 bound to the Cs-miR858 promoter and induced its expression, reducing PA accumulation in cucumber”
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.