Abstract
Present study was conducted to standardize a protocol for in vitro plant regeneration in Capsicum annuum L. cv California Wonder. Three different types of explants (Hypocotyl, Cotyledon and Cotyledonary petiole) from 8day-old seedling were used. Various concentrations of BA (2, 3 mgL-1), IBA (0.1, 0.2 mgL-1) and NAA (0.1, 0.2 mgL -1 ) were used for shoot regeneration. The highest number of shoot per explant was obtained from cotyledonary petiole on a MS medium supplemented with 3 mgL-1 BA and 0.2 mgL-1 IBA. Effective in vitro shoot elongation was achieved on half strength MS medium containing 0.1 mgL-1 BA in combination with 0.5 mgL-1 GA3. As well as adding activated charcoal (3 gL -1 ) and citric acid along with ascorbic acid caused eliminate phenolic material and prevent shoot necrosis in sweet pepper. Elongated shoots were rooted on half strength MS medium supplemented with 1 mgL-1 IBA.
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Claims 5
Claim · model-written summary
Cotyledonary petiole explants on MS medium with 3 mgL-1 BA and 0.2 mgL-1 IBA gave the highest number of shoots per explant
- Taxon
- sweet pepper
Capsicum annuumt0 - Organ / stage
- cotyledonary petiolee1
- Treatment
- MS medium supplemented with 3 mgL-1 BA and 0.2 mgL-1 IBAe1
- Outcome
- number of shoot per explante1
- Direction
- higheste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet pepper (Capsicum annuum L.)”
e1“The highest number of shoot per explant was obtained from cotyledonary petiole on a MS medium supplemented with 3 mgL-1 BA and 0.2 mgL-1 IBA”
Claim · model-written summary
Half strength MS medium with 0.1 mgL-1 BA and 0.5 mgL-1 GA3 was effective for in vitro shoot elongation
- Taxon
- sweet pepperCapsicum annuumt0
- Treatment
- half strength MS medium containing 0.1 mgL-1 BA in combination with 0.5 mgL-1 GA3e2
- Outcome
- shoot elongatione2
- Direction
- Effectivee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet pepper (Capsicum annuum L.)”
e2“Effective in vitro shoot elongation was achieved on half strength MS medium containing 0.1 mgL-1 BA in combination with 0.5 mgL-1 GA3”
Claim · model-written summary
Activated charcoal, citric acid and ascorbic acid prevented shoot necrosis in sweet pepper
- Taxon
- sweet pepperCapsicum annuumt0
- Treatment
- activated charcoal (3 gL -1 ) and citric acid along with ascorbic acide3
- Outcome
- shoot necrosise3
- Direction
- prevente3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet pepper (Capsicum annuum L.)”
e3“As well as adding activated charcoal (3 gL -1 ) and citric acid along with ascorbic acid caused eliminate phenolic material and prevent shoot necrosis in sweet pepper”
Claim · model-written summary
Activated charcoal, citric acid and ascorbic acid eliminated phenolic material in sweet pepper cultures
- Taxon
- sweet pepperCapsicum annuumt0
- Treatment
- activated charcoal (3 gL -1 ) and citric acid along with ascorbic acide3
- Outcome
- phenolic materiale3
- Direction
- eliminatee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet pepper (Capsicum annuum L.)”
e3“As well as adding activated charcoal (3 gL -1 ) and citric acid along with ascorbic acid caused eliminate phenolic material and prevent shoot necrosis in sweet pepper”
Claim · model-written summary
Elongated sweet pepper shoots were rooted on half strength MS medium with 1 mgL-1 IBA
- Taxon
- sweet pepperCapsicum annuumt0
- Outcome
- Elongated shoots were rootede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet pepper (Capsicum annuum L.)”
e4“Elongated shoots were rooted on half strength MS medium supplemented with 1 mgL-1 IBA”
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