Abstract
The application of modern biotechnology for improvement of pepper productivity requires an efficient in vitro plant regeneration protocol. In this study an experiment was carried out to examine the effects of different combinations of plant growth regulators on the in vitro micro propagation of Capsicum annuum L. thus optimizing the protocol for in vitro micro propagation involving culturing of nodal segments in MS media. Among the different concentrations of cytokinins BAP, KIN, Zea and TDZ tested. The best response was observed on medium containing 2 mg/l BAP and 0.5 mg/l IBA, especially for number of buds per explant and percentage of rooting. The elongated shoots were rooted on medium containing IBA (0.5 mg/l). Plantlets were transplanted to soil and acclimatized in the greenhouse showing normal development and growing to maturity bearing normal fruits with seeds.
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Claims 2
Claim · model-written summary
Medium with 2 mg/l BAP and 0.5 mg/l IBA gave the best response, for number of buds per explant and percentage of rooting, among the cytokinin concentrations tested in in vitro propagation of Capsicum annuum.
- Taxon
- Capsicum annuum L.
Capsicum annuume1 - Organ / stage
- nodal segmentse3
- Treatment
- medium containing 2 mg/l BAP and 0.5 mg/l IBAe0
- Outcome
- number of buds per explant and percentage of rootinge0
- Direction
- beste0
- Comparator
- different concentrations of cytokinins BAP, KIN, Zea and TDZe2
- Setting
- in vitroe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best response was observed on medium containing 2 mg/l BAP and 0.5 mg/l IBA, especially for number of buds per explant and percentage of rooting”
e1“in vitro micro propagation of Capsicum annuum L.”
e2“Among the different concentrations of cytokinins BAP, KIN, Zea and TDZ tested”
e3“culturing of nodal segments in MS media”
Claim · model-written summary
Plantlets transplanted to soil and acclimatised in the greenhouse showed normal development and grew to maturity bearing normal fruits with seeds.
- Taxon
- Capsicum annuum L.Capsicum annuume1
- Outcome
- normal development and growing to maturity bearing normal fruits with seedse0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plantlets were transplanted to soil and acclimatized in the greenhouse showing normal development and growing to maturity bearing normal fruits with seeds”
e1“in vitro micro propagation of Capsicum annuum L.”
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