Abstract
Pinellia ternata is a Chinese traditional medicinal herb, used to cure diseases including insomnia, eclampsia and cervical carcinoma, for hundreds of years. Non-self-recognition in multicellular organisms can initiate the innate immunity to avoid the invasion of pathogens. A design for pathogen independent, heterosis based, fresh resistance can be generated in F1 hybrid was proposed. By library functional screening, we found that P. ternata genes, named as ptHR375 and ptHR941, were identified with the potential to trigger a hypersensitive response in Nicotiana benthamiana. Significant induction of ROS and Callose deposition in N. benthamiana leaves along with activation of pathogenesis-related genes viz.; PR-1a, PR-5, PDF1.2, NPR1, PAL, RBOHB and ERF1 and antioxidant enzymes was observed. After transformation into N. benthamiana, expression of pathogenesis related genes was significantly up-regulated to generate high level of resistance against Phytophthora capsici without affecting the normal seed germination and morphological characters of the transformed N. benthamiana. UPLC-QTOF-MS analysis of ptHR375 transformed N. benthamiana revealed the induction of Oxytetracycline, Cuelure, Allantoin, Diethylstilbestrol and 1,2-Benzisothiazol-3(2H)-one as bioactive compounds. Here we also proved that F1 hybrids, produced by crossing of the ptHR375 and ptHR941 transformed and non-transformed N. benthamiana, show significant high levels of PR-gene expressions and pathogen resistance. Heterologous plant genes can activate disease resistance in another plant species and furthermore, by generating F1 hybrids, fresh pathogen independent plant immunity can be obtained. It is also concluded that ptHR375 and ptHR941 play their role in SA and JA/ET defense pathways to activate the resistance against invading pathogens.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
P. ternata genes ptHR375 and ptHR941 trigger a hypersensitive response in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Treatment
- P. ternata genese1
- Gene
- ptHR375e1
- Outcome
- hypersensitive responsee1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“P. ternata genes, named as ptHR375 and ptHR941, were identified with the potential to trigger a hypersensitive response in Nicotiana benthamiana”
Claim · model-written summary
Expression of P. ternata genes induced a significant increase in ROS and callose deposition in N. benthamiana leaves.
- Taxon
- N. benthamianaNicotiana benthamianae2
- Treatment
- P. ternata genese1
- Outcome
- ROS and Callose depositione2
- Direction
- increaseinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“P. ternata genes, named as ptHR375 and ptHR941, were identified with the potential to trigger a hypersensitive response in Nicotiana benthamiana”
e2“Significant induction of ROS and Callose deposition in N. benthamiana leaves along with activation of pathogenesis-related genes viz.; PR-1a, PR-5, PDF1.2, NPR1, PAL, RBOHB and ERF1 and antioxidant enzymes was observed”
Claim · model-written summary
P. ternata gene expression activated pathogenesis-related genes PR-1a, PR-5, PDF1.2, NPR1, PAL, RBOHB and ERF1 in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae2
- Treatment
- P. ternata genese1
- Gene
- PR-1a, PR-5, PDF1.2, NPR1, PAL, RBOHB and ERF1e2
- Outcome
- activation of pathogenesis-related genese2
- Direction
- increaseinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“P. ternata genes, named as ptHR375 and ptHR941, were identified with the potential to trigger a hypersensitive response in Nicotiana benthamiana”
e2“Significant induction of ROS and Callose deposition in N. benthamiana leaves along with activation of pathogenesis-related genes viz.; PR-1a, PR-5, PDF1.2, NPR1, PAL, RBOHB and ERF1 and antioxidant enzymes was observed”
Claim · model-written summary
P. ternata gene expression activated antioxidant enzymes in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae2
- Treatment
- P. ternata genese1
- Outcome
- antioxidant enzymese2
- Direction
- increaseinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“P. ternata genes, named as ptHR375 and ptHR941, were identified with the potential to trigger a hypersensitive response in Nicotiana benthamiana”
e2“Significant induction of ROS and Callose deposition in N. benthamiana leaves along with activation of pathogenesis-related genes viz.; PR-1a, PR-5, PDF1.2, NPR1, PAL, RBOHB and ERF1 and antioxidant enzymes was observed”
Claim · model-written summary
Transformation with the P. ternata genes generated a high level of resistance against Phytophthora capsici in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae3
- Treatment
- transformation into N. benthamianae3
- Interactor
- Phytophthora capsici
.jpg?width=60)
Phytophthora capsicie3pathogen - Outcome
- resistance against Phytophthora capsicie3
- Direction
- generate high levele3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e3“After transformation into N. benthamiana, expression of pathogenesis related genes was significantly up-regulated to generate high level of resistance against Phytophthora capsici without affecting the normal seed germination and morphological characters of the transformed N. benthamiana”
Claim · model-written summary
Transformation with the P. ternata genes did not affect normal seed germination or morphological characters of N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae3
- Treatment
- transformation into N. benthamianae3
- Outcome
- normal seed germination and morphological characterse3
- Direction
- without affectinge3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“After transformation into N. benthamiana, expression of pathogenesis related genes was significantly up-regulated to generate high level of resistance against Phytophthora capsici without affecting the normal seed germination and morphological characters of the transformed N. benthamiana”
Claim · model-written summary
UPLC-QTOF-MS of ptHR375-transformed N. benthamiana showed induction of Oxytetracycline, Cuelure and Allantoin.
- Taxon
- N. benthamianaNicotiana benthamianae4
- Treatment
- ptHR375 transformede4
- Compound
- Oxytetracycline, Cuelure, Allantoine4
- Outcome
- inductione4
- Direction
- inductione4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“UPLC-QTOF-MS analysis of ptHR375 transformed N. benthamiana revealed the induction of Oxytetracycline, Cuelure, Allantoin, Diethylstilbestrol and 1,2-Benzisothiazol-3(2H)-one as bioactive compounds”
Claim · model-written summary
UPLC-QTOF-MS of ptHR375-transformed N. benthamiana showed induction of Diethylstilbestrol and 1,2-Benzisothiazol-3(2H)-one.
- Taxon
- N. benthamianaNicotiana benthamianae4
- Treatment
- ptHR375 transformede4
- Compound
- Diethylstilbestrol and 1,2-Benzisothiazol-3(2H)-onee4
- Outcome
- inductione4
- Direction
- inductione4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“UPLC-QTOF-MS analysis of ptHR375 transformed N. benthamiana revealed the induction of Oxytetracycline, Cuelure, Allantoin, Diethylstilbestrol and 1,2-Benzisothiazol-3(2H)-one as bioactive compounds”
Claim · model-written summary
F1 hybrids of transformed and non-transformed N. benthamiana showed significantly high levels of PR-gene expression and pathogen resistance.
- Taxon
- N. benthamianaNicotiana benthamianae5
- Material
- F1 hybridse5
- Treatment
- crossing of the ptHR375 and ptHR941 transformed and non-transformed N. benthamianae5
- Outcome
- PR-gene expressions and pathogen resistancee5
- Direction
- high levelse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“F1 hybrids, produced by crossing of the ptHR375 and ptHR941 transformed and non-transformed N. benthamiana, show significant high levels of PR-gene expressions and pathogen resistance”
Claim · model-written summary
ptHR375 and ptHR941 are concluded to act through SA and JA/ET defence pathways to activate resistance against invading pathogens.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- ptHR375 and ptHR941e6
- Outcome
- resistance against invading pathogense6
- Qualifier
- SA and JA/ET defense pathwayse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e6“ptHR375 and ptHR941 play their role in SA and JA/ET defense pathways to activate the resistance against invading pathogens”
The same questions in other papers
transgenic · growth · Nicotiana benthamianaPositive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
2 papers more in this group
See all 6 papers →transgenic · Phytophthora capsici · Nicotiana benthamianaPositive and negative results
4 papers in the same direction as this paper · 11 in the opposite direction
- negative
- negative
- negative
12 papers more in this group
See all 16 papers →transgenic · disease · Nicotiana benthamianaPositive and negative results
25 papers in the same direction as this paper · 27 in the opposite direction
- negative
- negative
- negative
49 papers more in this group
See all 53 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.