Abstract
Kunitz-type (PKPI) and Potato type I (Pin1) protease inhibitors (PIs) are two families of serine proteinase inhibitors often associated to plant storage organs and with well known insecticidal and nematicidal activities. Noteworthy, their ability to limit fungal and bacterial pathogenesis in vivo or to influence plant physiology has not been investigated in detail. To this aim, we generated a set of PVX-based viral constructs to transiently and heterologously express two potato PKPI (PKI1, PKI2) and three potato Pin1 (PPI3A2, PPI3B2, PPI2C4) genes in Nicotiana benthamiana plants, a widely used model for plant-pathogen interaction studies. Interestingly, transgenic plants expressing most of the tested PIs showed to be highly resistant against two economically important necrotrophic fungal pathogens, Botrytis cinerea and Alternaria alternata. Unexpectedly, overexpression of the PKI2 Kunitz-type or of the PPI2C4 and PPI3A2 Potato type I inhibitor genes also lead to a dramatic reduction in the propagation and symptom development produced by the bacterial pathogen Pseudomonas syringae. We further found that localized expression of PPI2C4 and PKI2 in N. benthamiana leaves caused an increase in cell expansion and proliferation which lead to tissue hypertrophy and trichome accumulation. In line with this, the systemic expression of these proteins resulted in plants with enhanced shoot and root biomass. Collectively, our results indicate that PKPI and Pin1 PIs might represent valuable tools to simultaneously increase plant fitness and broad-spectrum resistance towards phytopathogens.
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Claims 7
Claim · model-written summary
Transgenic N. benthamiana expressing most tested PIs was highly resistant to Botrytis cinerea.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- transgenic plants expressing most of the tested PIse1
- Interactor
- Botrytis cinerea
Botrytis cinereae1pathogen - Outcome
- highly resistante1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in Nicotiana benthamiana plants”
e1“transgenic plants expressing most of the tested PIs showed to be highly resistant against two economically important necrotrophic fungal pathogens, Botrytis cinerea and Alternaria alternata”
Claim · model-written summary
Transgenic N. benthamiana expressing most tested PIs was highly resistant to Alternaria alternata.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- transgenic plants expressing most of the tested PIse1
- Interactor
- Alternaria alternata
.jpg?width=60)
Alternaria alternatae1pathogen - Outcome
- highly resistante1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“in Nicotiana benthamiana plants”
e1“transgenic plants expressing most of the tested PIs showed to be highly resistant against two economically important necrotrophic fungal pathogens, Botrytis cinerea and Alternaria alternata”
Claim · model-written summary
Overexpression of PKI2 or of PPI2C4 and PPI3A2 greatly reduced propagation and symptom development of Pseudomonas syringae.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- overexpression of the PKI2 Kunitz-type or of the PPI2C4 and PPI3A2 Potato type I inhibitor genese2
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee2pathogen - Gene
- PKI2e2
- Outcome
- propagation and symptom developmente2
- Direction
- reductione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in Nicotiana benthamiana plants”
e2“overexpression of the PKI2 Kunitz-type or of the PPI2C4 and PPI3A2 Potato type I inhibitor genes also lead to a dramatic reduction in the propagation and symptom development produced by the bacterial pathogen Pseudomonas syringae”
Claim · model-written summary
Localized expression of PPI2C4 and PKI2 in N. benthamiana leaves increased cell expansion and proliferation.
- Taxon
- N. benthamianaNicotiana benthamianae3
- Organ / stage
- leavese3
- Treatment
- localized expression of PPI2C4 and PKI2 in N. benthamiana leavese3
- Outcome
- cell expansion and proliferatione3
- Direction
- increasee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“localized expression of PPI2C4 and PKI2 in N. benthamiana leaves caused an increase in cell expansion and proliferation which lead to tissue hypertrophy and trichome accumulation”
Claim · model-written summary
Localized expression of PPI2C4 and PKI2 in N. benthamiana leaves led to tissue hypertrophy and trichome accumulation.
- Taxon
- N. benthamianaNicotiana benthamianae3
- Organ / stage
- leavese3
- Treatment
- localized expression of PPI2C4 and PKI2 in N. benthamiana leavese3
- Outcome
- tissue hypertrophy and trichome accumulatione3
- Direction
- increaseinferrede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“localized expression of PPI2C4 and PKI2 in N. benthamiana leaves caused an increase in cell expansion and proliferation which lead to tissue hypertrophy and trichome accumulation”
Claim · model-written summary
Systemic expression of the PIs gave plants with enhanced shoot and root biomass.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- systemic expression of these proteinse4
- Outcome
- shoot and root biomasse4
- Direction
- enhancede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in Nicotiana benthamiana plants”
e4“the systemic expression of these proteins resulted in plants with enhanced shoot and root biomass”
Claim · model-written summary
PKPI and Pin1 inhibitors might be valuable tools to increase plant fitness and broad-spectrum resistance.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Outcome
- plant fitness and broad-spectrum resistancee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in Nicotiana benthamiana plants”
e5“PKPI and Pin1 PIs might represent valuable tools to simultaneously increase plant fitness and broad-spectrum resistance towards phytopathogens”
The same questions in other papers
transgenic · growth · Nicotiana benthamianaPositive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- negative
- no effect
- no effect
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.