Abstract
SDE1/SGS2/RDR6, a putative RNA-dependent RNA polymerase (RdRP) from Arabidopsis thaliana, has previously been found to be indispensable for maintaining the posttranscriptional silencing of transgenes, but it is seemingly redundant for antiviral defense. To elucidate the antiviral role of this RdRP in a different host plant and to evaluate whether plant growth conditions affect its role, we down-regulated expression of the Nicotiana benthamiana homolog, NbRDR6, and examined the plants for altered susceptibility to various viruses at different growth temperatures. The results we describe here clearly show that plants with reduced expression of NbRDR6 were more susceptible to all viruses tested and that this effect was more pronounced at higher growth temperatures. Diminished expression of NbRDR6 also permitted efficient multiplication of tobacco mosaic virus in the shoot apices, leading to serious disruption with microRNA-mediated developmental regulation. Based on these results, we propose that NbRDR6 participates in the antiviral RNA silencing pathway that is stimulated by rising temperatures but suppressed by virus-encoded silencing suppressors. The relative strengths of these two factors, along with other plant defense components, critically influence the outcome of virus infections.
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Claims 3
Claim · model-written summary
Reduced NbRDR6 expression increased susceptibility to all tested viruses, especially at higher growth temperatures.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Treatment
- reduced expression of NbRDR6e0
- Gene
- NbRDR6e0
- Outcome
- susceptible to all viruses testede0
- Direction
- more susceptiblee0
- Qualifier
- higher growth temperaturese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“plants with reduced expression of NbRDR6 were more susceptible to all viruses tested and that this effect was more pronounced at higher growth temperatures”
Claim · model-written summary
Diminished NbRDR6 expression allowed efficient tobacco mosaic virus multiplication in shoot apices.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Organ / stage
- shoot apicese0
- Treatment
- Diminished expression of NbRDR6e0
- Interactor
- tobacco mosaic virus
Tobacco mosaic viruse0pathogen - Gene
- NbRDR6e0
- Outcome
- multiplication of tobacco mosaic viruse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“Diminished expression of NbRDR6 also permitted efficient multiplication of tobacco mosaic virus in the shoot apices”
Claim · model-written summary
Tobacco mosaic virus multiplication in shoot apices led to serious disruption of microRNA-mediated developmental regulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Organ / stage
- shoot apicese0
- Interactor
- tobacco mosaic virusTobacco mosaic viruse0pathogen
- Outcome
- serious disruption with microRNA-mediated developmental regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“multiplication of tobacco mosaic virus in the shoot apices, leading to serious disruption with microRNA-mediated developmental regulation”
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See all 53 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.