Abstract
SUMMARY Pathogens secrete a large number of effectors that manipulate host processes to create an environment conducive to pathogen colonization. However, the underlying mechanisms by which Plasmopara viticola effectors manipulate host plant cells remain largely unclear. In this study, we reported that RXLR31154 , a P. viticola RXLR effector, was highly expressed during the early stages of P. viticola infection. In our study, stable expression of RXLR31154 in grapevine ( Vitis vinifera ) and Nicotiana benthamiana promoted leaf colonization by P . viticola and Phytophthora capsici , respectively. By yeast two‐hybrid screening, the 23‐kDa oxygen‐evolving enhancer 2 (VpOEE2 or VpPsbP), encoded by the PsbP gene, in V itis piasezkii accession Liuba‐8 was identified as a host target of RXLR31154. Overexpression of VpPsbP enhanced susceptibility to P. viticola in grapevine and P. capsici in N. benthamiana , and silencing of NbPsbPs , the homologs of PsbP in N. benthamiana , reduced P. capcisi colonization, indicating that PsbP is a susceptibility factor. RXLR31154 and VpPsbP protein were co‐localized in the chloroplast. Moreover, VpPsbP reduced H 2 O 2 accumulation and activated the 1 O 2 signaling pathway in grapevine. RXLR31154 could stabilize PsbP. Together, our data revealed that RXLR31154 reduces H 2 O 2 accumulation and activates the 1 O 2 signaling pathway through stabilizing PsbP, thereby promoting disease.
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Claims 12
Claim · model-written summary
RXLR31154 was highly expressed early in P. viticola infection.
- Interactor
- P. viticola
Plasmopara viticolae0pathogen - Gene
- RXLR31154e0
- Outcome
- RXLR31154e0
- Direction
- highly expressede0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RXLR31154 , a P. viticola RXLR effector, was highly expressed during the early stages of P. viticola infection”
t0“grapevine”
Claim · model-written summary
Stable RXLR31154 expression promoted P. viticola leaf colonization in grapevine.
- Treatment
- stable expression of RXLR31154e0
- Interactor
- P . viticolaPlasmopara viticolae0pathogen
- Gene
- RXLR31154e0
- Outcome
- leaf colonization by P . viticolae0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stable expression of RXLR31154 in grapevine ( Vitis vinifera ) and Nicotiana benthamiana promoted leaf colonization by P . viticola and Phytophthora capsici , respectively”
Claim · model-written summary
Stable RXLR31154 expression promoted Phytophthora capsici leaf colonization in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- stable expression of RXLR31154e0
- Interactor
- Phytophthora capsici
.jpg?width=60)
Phytophthora capsicie0pathogen - Gene
- RXLR31154e0
- Outcome
- leaf colonization by P . viticola and Phytophthora capsicie0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“stable expression of RXLR31154 in grapevine ( Vitis vinifera ) and Nicotiana benthamiana promoted leaf colonization by P . viticola and Phytophthora capsici , respectively”
Claim · model-written summary
VpPsbP in Vitis piasezkii was identified as a host target of RXLR31154.
Verbatim
e0“the 23‐kDa oxygen‐evolving enhancer 2 (VpOEE2 or VpPsbP), encoded by the PsbP gene, in V itis piasezkii accession Liuba‐8 was identified as a host target of RXLR31154”
Claim · model-written summary
VpPsbP overexpression enhanced P. viticola susceptibility in grapevine.
- Treatment
- Overexpression of VpPsbPe0
- Interactor
- P. viticolaPlasmopara viticolae0pathogen
- Gene
- VpPsbPe0
- Outcome
- susceptibility to P. viticolae0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of VpPsbP enhanced susceptibility to P. viticola in grapevine and P. capsici in N. benthamiana”
Claim · model-written summary
VpPsbP overexpression enhanced P. capsici susceptibility in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- Overexpression of VpPsbPe0
- Interactor
- P. capsiciPhytophthora capsicie0pathogen
- Gene
- VpPsbPe0
- Outcome
- susceptibility to P. viticola in grapevine and P. capsicie0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of VpPsbP enhanced susceptibility to P. viticola in grapevine and P. capsici in N. benthamiana”
Claim · model-written summary
NbPsbPs silencing reduced P. capcisi colonization in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- silencing of NbPsbPse0
- Interactor
- P. capcisie0pathogen
- Gene
- NbPsbPse0
- Outcome
- P. capcisi colonizatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of NbPsbPs , the homologs of PsbP in N. benthamiana , reduced P. capcisi colonization”
Claim · model-written summary
RXLR31154 and VpPsbP colocalized in chloroplasts.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RXLR31154 and VpPsbP protein were co‐localized in the chloroplast”
t0“grapevine”
Claim · model-written summary
VpPsbP reduced H2O2 accumulation in grapevine.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VpPsbP reduced H 2 O 2 accumulation and activated the 1 O 2 signaling pathway in grapevine”
Claim · model-written summary
VpPsbP activated 1O2 signaling in grapevine.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VpPsbP reduced H 2 O 2 accumulation and activated the 1 O 2 signaling pathway in grapevine”
Claim · model-written summary
RXLR31154 stabilized PsbP.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RXLR31154 could stabilize PsbP”
t0“grapevine”
Claim · model-written summary
RXLR31154 promoted disease through PsbP stabilization and altered ROS signaling.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RXLR31154 reduces H 2 O 2 accumulation and activates the 1 O 2 signaling pathway through stabilizing PsbP, thereby promoting disease”
t0“grapevine”
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See all 143 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.