Abstract
Stomatal movement plays an essential role in plant responses to drought stress, and the actin cytoskeleton and abscisic acid (ABA) are two important components of this process. Little is known about the mechanism underlying actin cytoskeleton remodeling and the dynamic changes occurring during stomatal movement in response to drought stress/ABA signaling. Actin-depolymerizing factors (ADFs) are conserved actin severing/depolymerizing proteins in eukaryotes, and in angiosperms ADFs have evolved actin-bundling activity. Here, we reveal that the transcriptional expression of neofunctionalized Arabidopsis ADF5 was induced by drought stress and ABA treatment. Furthermore, we demonstrated that ADF5 loss-of-function mutations increased water loss from detached leaves, reduced plant survival rates after drought stress, and delayed stomatal closure by regulating actin cytoskeleton remodeling via its F-actin-bundling activity. Biochemical assays revealed that an ABF/AREB transcription factor, DPBF3, could bind to the ADF5 promoter and activate its transcription via the ABA-responsive element core motif ACGT/C. Taken together, our findings indicate that ADF5 participates in drought stress by regulating stomatal closure, and may also serve as a potential downstream target of the drought stress/ABA signaling pathway via members of the ABF/AREB transcription factors family.
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Claims 6
Claim · model-written summary
Transcript expression of Arabidopsis ADF5 was induced by drought stress.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- drought stresse0
- Gene
- Arabidopsis ADF5e0
- Outcome
- transcriptional expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcriptional expression of neofunctionalized Arabidopsis ADF5 was induced by drought stress and ABA treatment”
Claim · model-written summary
Transcript expression of Arabidopsis ADF5 was induced by ABA treatment.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- ABA treatmente0
- Gene
- Arabidopsis ADF5e0
- Outcome
- transcriptional expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcriptional expression of neofunctionalized Arabidopsis ADF5 was induced by drought stress and ABA treatment”
Claim · model-written summary
ADF5 loss-of-function mutations increased water loss from detached leaves in Arabidopsis.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- detached leavese0
- Treatment
- ADF5 loss-of-function mutationse0
- Gene
- ADF5e0
- Outcome
- water loss from detached leavese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis ADF5”
e0“ADF5 loss-of-function mutations increased water loss from detached leaves”
Claim · model-written summary
ADF5 loss-of-function mutations reduced plant survival rates after drought stress in Arabidopsis.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ADF5 loss-of-function mutationse0
- Gene
- ADF5e0
- Outcome
- plant survival rates after drought stresse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis ADF5”
e0“ADF5 loss-of-function mutations increased water loss from detached leaves, reduced plant survival rates after drought stress”
Claim · model-written summary
ADF5 loss-of-function mutations delayed stomatal closure, by regulating actin cytoskeleton remodeling via its F-actin-bundling activity.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ADF5 loss-of-function mutationse0
- Gene
- ADF5e0
- Outcome
- stomatal closuree0
- Direction
- delayede0
- Qualifier
- regulating actin cytoskeleton remodeling via its F-actin-bundling activitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis ADF5”
e0“ADF5 loss-of-function mutations increased water loss from detached leaves, reduced plant survival rates after drought stress, and delayed stomatal closure by regulating actin cytoskeleton remodeling via its F-actin-bundling activity”
Claim · model-written summary
The transcription factor DPBF3 bound the ADF5 promoter and activated its transcription via the ABA-responsive element core motif ACGT/C.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- DPBF3e0
- Outcome
- bind to the ADF5 promoter and activate its transcriptione0
- Qualifier
- ABA-responsive element core motif ACGT/Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis ADF5”
e0“DPBF3, could bind to the ADF5 promoter and activate its transcription via the ABA-responsive element core motif ACGT/C”
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