Abstract
Treatment of cell suspension cultures of bean (Phaseolus vulgaris c.v. Immuna) with an elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum resulted in rapid changes in the composition of the bean cell walls. These consisted of (a) increases in phenolic material bound to the cellulosic and hemicellulosic fractions of the wall, (b) loss of material (mainly glucose) from the hemicellulosic fraction and (c) an increase in wall-associated hydroxyproline. The increases in wall-bound phenolics were preceded by (a) rapid decreases in the intracellular levels of free hydroxycinnamic acids and (b) transient increases in the extractable activities of L-phenylalanine ammonia-lyase and cinnamic acid 4-hydroxylase. 4-Hydroxycinnamic acid 3-hydroxylase activity was present at a high level in control cultures and was not induced by elicitor. Changes in the levels of cytochrome P-450, as determined by dot blot assays utilising an anti-(P-450) monoclonal antibody, paralleled the changes in cinnamic acid 4-hydroxylase activity. The accumulation of cell wall hydroxyproline was associated with rapid transient increases in the extractable activities of proline 2-oxoglutarate dioxygenase and a protein arabinosyl transferase. An hydroxyproline-rich acceptor protein of Mr 42 500 was the major protein to incorporate [3H]arabinose following elicitation of the bean cells, and the kinetics of the extent of labelling of this protein paralleled the accumulation of hydroxyproline protein in the endomembrane system. The above metabolic changes associated with cell wall components followed rapid kinetics similar to those involved in the formation of the phytoalexin kievitone in the elicited cultures [Robbins, M. P. et al. (1985) Eur. J. Biochem. 148, 563-569]. It is therefore concluded that increased 5-hydroxy-substituted isoflavonoid biosynthesis, wall-bound phenolic synthesis and synthesis of arabinosylated hydroxyproline-rich protein are all early events which are closely linked to the initial interaction between plant cell and fungal elicitor.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
Fungal elicitor increased phenolic material in bean cell-wall fractions.
- Taxon
- beane0
- Material
- Immunae0
- Treatment
- elicitor preparatione0
- Interactor
- Colletotrichum lindemuthianum
Colletotrichum lindemuthianume0pathogen - Outcome
- phenolic material bound to the cellulosic and hemicellulosic fractions of the walle1
- Direction
- increasese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of cell suspension cultures of bean (Phaseolus vulgaris c.v. Immuna) with an elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum”
e1“increases in phenolic material bound to the cellulosic and hemicellulosic fractions of the wall”
Claim · model-written summary
Elicitor treatment caused loss of mainly glucose material from bean hemicellulose.
- Taxon
- beane0
- Treatment
- elicitor preparatione0
- Interactor
- Colletotrichum lindemuthianumColletotrichum lindemuthianume0pathogen
- Outcome
- material (mainly glucose) from the hemicellulosic fractione1
- Direction
- losse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of cell suspension cultures of bean (Phaseolus vulgaris c.v. Immuna) with an elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum”
e1“loss of material (mainly glucose) from the hemicellulosic fraction”
Claim · model-written summary
Elicitor treatment increased wall-associated hydroxyproline in bean cells.
- Taxon
- beane0
- Treatment
- elicitor preparatione0
- Outcome
- wall-associated hydroxyprolinee1
- Direction
- increasee1
Not reportedComparator
Verbatim
e0“Treatment of cell suspension cultures of bean (Phaseolus vulgaris c.v. Immuna) with an elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum”
e1“an increase in wall-associated hydroxyproline”
Claim · model-written summary
Free intracellular hydroxycinnamic acids rapidly decreased before wall-bound phenolics rose.
Verbatim
e0“bean (Phaseolus vulgaris c.v. Immuna)”
e1“rapid decreases in the intracellular levels of free hydroxycinnamic acids”
Claim · model-written summary
L-phenylalanine ammonia-lyase and cinnamic acid 4-hydroxylase activities rose transiently before wall-bound phenolics increased.
Verbatim
e0“bean (Phaseolus vulgaris c.v. Immuna)”
e1“transient increases in the extractable activities of L-phenylalanine ammonia-lyase and cinnamic acid 4-hydroxylase”
Claim · model-written summary
4-Hydroxycinnamic acid 3-hydroxylase activity was high in controls and not induced by elicitor.
Verbatim
e0“bean (Phaseolus vulgaris c.v. Immuna)”
e1“4-Hydroxycinnamic acid 3-hydroxylase activity was present at a high level in control cultures and was not induced by elicitor”
Claim · model-written summary
Cytochrome P-450 levels paralleled cinnamic acid 4-hydroxylase activity.
Not reportedDirection
Verbatim
e0“bean (Phaseolus vulgaris c.v. Immuna)”
e1“Changes in the levels of cytochrome P-450, as determined by dot blot assays utilising an anti-(P-450) monoclonal antibody, paralleled the changes in cinnamic acid 4-hydroxylase activity”
Claim · model-written summary
Wall hydroxyproline accumulation was associated with transient increases in proline 2-oxoglutarate dioxygenase and protein arabinosyl transferase activities.
Verbatim
e0“bean (Phaseolus vulgaris c.v. Immuna)”
e1“The accumulation of cell wall hydroxyproline was associated with rapid transient increases in the extractable activities of proline 2-oxoglutarate dioxygenase and a protein arabinosyl transferase”
Claim · model-written summary
A 42,500-Mr hydroxyproline-rich protein was the main acceptor of labelled arabinose after elicitation.
- Taxon
- beane0
- Gene
- hydroxyproline-rich acceptor proteine1
- Outcome
- major protein to incorporate [3H]arabinosee1
- Value
- Mr 42 500e1
Not reportedDirection
Verbatim
e0“bean (Phaseolus vulgaris c.v. Immuna)”
e1“An hydroxyproline-rich acceptor protein of Mr 42 500 was the major protein to incorporate [3H]arabinose following elicitation of the bean cells”
Claim · model-written summary
Labelling kinetics of the acceptor protein paralleled hydroxyproline protein accumulation in the endomembrane system.
- Taxon
- beane0
- Organ / stage
- endomembrane systeme1
- Outcome
- kinetics of the extent of labelling of this proteine1
Not reportedDirection
Verbatim
e0“bean (Phaseolus vulgaris c.v. Immuna)”
e1“the kinetics of the extent of labelling of this protein paralleled the accumulation of hydroxyproline protein in the endomembrane system”
Claim · model-written summary
The measured cell-wall metabolic changes followed rapid kinetics similar to phytoalexin kievitone formation.
- Taxon
- beane0
- Compound
- phytoalexin kievitonee1
- Outcome
- metabolic changes associated with cell wall componentse1
Not reportedDirection
Verbatim
e0“bean (Phaseolus vulgaris c.v. Immuna)”
e1“The above metabolic changes associated with cell wall components followed rapid kinetics similar to those involved in the formation of the phytoalexin kievitone in the elicited cultures”
Claim · model-written summary
The authors conclude that increased isoflavonoid, wall-bound phenolic, and arabinosylated protein synthesis are early events linked to fungal elicitation.
- Taxon
- beane0
- Outcome
- early events which are closely linked to the initial interaction between plant cell and fungal elicitore1
- Qualifier
- increased 5-hydroxy-substituted isoflavonoid biosynthesis, wall-bound phenolic synthesis and synthesis of arabinosylated hydroxyproline-rich proteine1
Not reportedDirection
Verbatim
e0“bean (Phaseolus vulgaris c.v. Immuna)”
e1“increased 5-hydroxy-substituted isoflavonoid biosynthesis, wall-bound phenolic synthesis and synthesis of arabinosylated hydroxyproline-rich protein are all early events which are closely linked to the initial interaction between plant cell and fungal elicitor”
The same questions in other papers
resistance inducer · Colletotrichum lindemuthianum · Phaseolus vulgaris (common bean)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →resistance inducer · molecular: metabolites · Phaseolus vulgaris (common bean)Positive and negative results
1 paper in the same direction as this paper · 11 in the opposite direction
- positive
- positive
- positive
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.