Abstract
Changes in the activity levels of mRNAs encoding phenylalanine ammonia-lyase and chalcone synthase, two characteristic enzymes of phenylpropanoid biosynthesis, in elicitor-treated cells of dwarf French bean (Phaseolus vulgaris L.) have been investigated by immunoprecipitation of [35S]methionine-labelled enzyme subunits synthesised in vitro in an mRNA-dependent rabbit reticulocyte lysate translation system. Elicitor heat-released from cell walls of Colletotrichum lindemuthianum, the causal agent of anthracnose disease of bean, causes marked rapid increases in the polysomal activities of the mRNAs encoding the two enzymes concomitant with the phase of rapid increase in enzyme activity at the onset of phaseollin accumulation during the phytoalexin defence response. Increased polysomal mRNA activities encoding the two enzymes can be observed 30 min after elicitor treatment. The patterns of induction of the mRNA activities are broadly similar with respect to time and elicitor concentration although small but distinct differences between the enzymes were observed in the elicitor concentration giving maximum induction. There is a close correlation between the induction of polysomal mRNA activity and the induction of enzyme synthesis in vivo by elicitor treatment with respect to both the kinetics of induction and the dependence on elicitor concentration. The data indicate that elicitor stimulation of phenylalanine ammonia-lyase and chalcone synthase synthesis in vivo is largely a result of increased polysomal activity of the mRNAs encoding these enzymes. Similar patterns of induction of polysomal mRNA activity are observed with elicitor preparations from a variety of sources. The marked increases in polysomal mRNA activities encoding phenylalanine ammonia-lyase and chalcone synthase are increases as a proportion of total cellular mRNA activity, indicating that elicitor does not increase these polysomal mRNA activities by stimulation of selective recruitment from the total pool of cellular mRNA.
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Claims 9
Claim · model-written summary
Fungal elicitor rapidly increased polysomal mRNA activities encoding phenylalanine ammonia-lyase and chalcone synthase.
- Taxon
- dwarf French bean
Phaseolus vulgarise1 - Treatment
- Elicitor heat-released from cell walls of Colletotrichum lindemuthianume0
- Interactor
- Colletotrichum lindemuthianum
Colletotrichum lindemuthianume0pathogen - Gene
- phenylalanine ammonia-lyase and chalcone synthasee1
- Outcome
- polysomal activities of the mRNAse0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elicitor heat-released from cell walls of Colletotrichum lindemuthianum, the causal agent of anthracnose disease of bean, causes marked rapid increases in the polysomal activities of the mRNAs encoding the two enzymes”
e1“phenylalanine ammonia-lyase and chalcone synthase, two characteristic enzymes of phenylpropanoid biosynthesis, in elicitor-treated cells of dwarf French bean (Phaseolus vulgaris L.)”
Claim · model-written summary
Increased polysomal mRNA activities were observable 30 minutes after elicitor treatment.
- Taxon
- dwarf French beanPhaseolus vulgarise1
- Outcome
- polysomal mRNA activitiese0
- Direction
- Increasede0
- Qualifier
- 30 min after elicitor treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increased polysomal mRNA activities encoding the two enzymes can be observed 30 min after elicitor treatment”
e1“dwarf French bean (Phaseolus vulgaris L.)”
Claim · model-written summary
The two enzyme mRNA induction patterns were broadly similar over time and elicitor concentration.
- Taxon
- dwarf French beanPhaseolus vulgarise1
- Outcome
- patterns of induction of the mRNA activitiese0
- Qualifier
- time and elicitor concentratione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The patterns of induction of the mRNA activities are broadly similar with respect to time and elicitor concentration”
e1“dwarf French bean (Phaseolus vulgaris L.)”
Claim · model-written summary
The enzymes differed slightly in elicitor concentration for maximum induction.
- Taxon
- dwarf French beanPhaseolus vulgarise1
- Outcome
- differences between the enzymese0
- Qualifier
- elicitor concentration giving maximum inductione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“small but distinct differences between the enzymes were observed in the elicitor concentration giving maximum induction”
e1“dwarf French bean (Phaseolus vulgaris L.)”
Claim · model-written summary
Polysomal mRNA activity induction correlated closely with enzyme synthesis induction over kinetics and elicitor concentration.
- Taxon
- dwarf French beanPhaseolus vulgarise1
- Treatment
- elicitor treatmente0
- Outcome
- induction of enzyme synthesis in vivoe0
- Qualifier
- close correlation between the induction of polysomal mRNA activity and the induction of enzyme synthesise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There is a close correlation between the induction of polysomal mRNA activity and the induction of enzyme synthesis in vivo by elicitor treatment with respect to both the kinetics of induction and the dependence on elicitor concentration”
e1“dwarf French bean (Phaseolus vulgaris L.)”
Claim · model-written summary
The authors inferred that increased polysomal mRNA activity largely explains elicitor-stimulated enzyme synthesis.
- Taxon
- dwarf French beanPhaseolus vulgarise1
- Treatment
- elicitor stimulatione0
- Outcome
- phenylalanine ammonia-lyase and chalcone synthase synthesis in vivoe0
- Qualifier
- increased polysomal activity of the mRNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The data indicate that elicitor stimulation of phenylalanine ammonia-lyase and chalcone synthase synthesis in vivo is largely a result of increased polysomal activity of the mRNAs encoding these enzymes”
e1“dwarf French bean (Phaseolus vulgaris L.)”
Claim · model-written summary
Elicitors from various sources produced similar polysomal mRNA induction patterns.
- Taxon
- dwarf French beanPhaseolus vulgarise1
- Treatment
- elicitor preparations from a variety of sourcese0
- Outcome
- patterns of induction of polysomal mRNA activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similar patterns of induction of polysomal mRNA activity are observed with elicitor preparations from a variety of sources”
e1“dwarf French bean (Phaseolus vulgaris L.)”
Claim · model-written summary
The induced enzyme-encoding polysomal mRNA activities rose as a proportion of total cellular mRNA activity.
- Taxon
- dwarf French beanPhaseolus vulgarise1
- Gene
- phenylalanine ammonia-lyase and chalcone synthasee0
- Outcome
- polysomal mRNA activitiese0
- Direction
- increasese0
- Qualifier
- as a proportion of total cellular mRNA activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The marked increases in polysomal mRNA activities encoding phenylalanine ammonia-lyase and chalcone synthase are increases as a proportion of total cellular mRNA activity”
e1“dwarf French bean (Phaseolus vulgaris L.)”
Claim · model-written summary
The authors inferred that selective recruitment from total cellular mRNA does not explain the induced polysomal activities.
- Taxon
- dwarf French beanPhaseolus vulgarise1
- Outcome
- polysomal mRNA activitiese0
- Qualifier
- selective recruitment from the total pool of cellular mRNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elicitor does not increase these polysomal mRNA activities by stimulation of selective recruitment from the total pool of cellular mRNA”
e1“dwarf French bean (Phaseolus vulgaris L.)”
The same questions in other papers
Colletotrichum lindemuthianum · molecular: metabolites · Phaseolus vulgaris (common bean)Results agree
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- positive
- positive
- positive
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7 papers in the same direction as this paper · 3 in the opposite direction
- negative
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See all 11 papers →resistance inducer · molecular: metabolites · Phaseolus vulgaris (common bean)Positive and negative results
10 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- positive
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.