Abstract
Treatment of cell suspension cultures of Phaseolus vulgaris c.v. Immuna with an elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum resulted in rapid accumulation of the prenylated 5-hydroxyisoflavanone phytoalexin kievitone followed by later accumulation of the pterocarpan-derived phytoalexin phaseollin. Kievitone formation was preceded by rapid transient increases in the extractable activities of the enzymes L-phenylalanine ammonia-lyase and chalcone synthase. The extractable activities of 15 enzymes were measured in the cell cultures during the period of kievitone accumulation. The results suggest a highly selective induction of enzymes associated directly with the phytoalexin pathway. No induction of enzymes of pathways diverging from or providing substrates for the phenylpropanoid----isoflavonoid pathway was observed. The increase in glutamate dehydrogenase activity in control cultures was prevented by elicitor application. A comparison of enzyme activities in control and Colletotrichum-infected bean hypocotyls provided further evidence of the selective induction of enzymes of phytoalexin synthesis, although peroxidase, glutamate dehydrogenase and glutamate synthase activities were higher in infected than in healthy hypocotyls. It is concluded that the major enzymic changes occurring in elicitor-treated bean cells are probably those directly associated with defence mechanisms such as the formation of isoflavonoid phytoalexins (this paper) or accumulation of phenolic compounds and hydroxyproline-protein in the cell walls [Bolwell, G. P. et al. (1985) Eur. J. Biochem. 148, 571-578].
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
The elicitor preparation from Colletotrichum lindemuthianum caused rapid accumulation of the phytoalexin kievitone in Phaseolus vulgaris cell cultures.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarise0 - Material
- Immunae0
- Treatment
- elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianume0
- Interactor
- Colletotrichum lindemuthianum
Colletotrichum lindemuthianume0pathogen - Outcome
- rapid accumulation of the prenylated 5-hydroxyisoflavanone phytoalexin kievitonee0
- Direction
- increaseinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of cell suspension cultures of Phaseolus vulgaris c.v. Immuna with an elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum resulted in rapid accumulation of the prenylated 5-hydroxyisoflavanone phytoalexin kievitone followed by later accumulation of the pterocarpan-derived phytoalexin phaseollin”
Claim · model-written summary
The elicitor preparation from Colletotrichum lindemuthianum caused later accumulation of the phytoalexin phaseollin in Phaseolus vulgaris cell cultures.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Material
- Immunae0
- Treatment
- elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianume0
- Interactor
- Colletotrichum lindemuthianumColletotrichum lindemuthianume0pathogen
- Outcome
- later accumulation of the pterocarpan-derived phytoalexin phaseolline0
- Direction
- increaseinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of cell suspension cultures of Phaseolus vulgaris c.v. Immuna with an elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum resulted in rapid accumulation of the prenylated 5-hydroxyisoflavanone phytoalexin kievitone followed by later accumulation of the pterocarpan-derived phytoalexin phaseollin”
Claim · model-written summary
Rapid transient increases in the activities of L-phenylalanine ammonia-lyase and chalcone synthase preceded kievitone formation in elicitor-treated bean cells.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Treatment
- elicitore0
- Gene
- L-phenylalanine ammonia-lyase and chalcone synthasee1
- Outcome
- extractable activities of the enzymese1
- Direction
- increasese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of cell suspension cultures of Phaseolus vulgaris c.v. Immuna with an elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum resulted in rapid accumulation of the prenylated 5-hydroxyisoflavanone phytoalexin kievitone followed by later accumulation of the pterocarpan-derived phytoalexin phaseollin”
e1“Kievitone formation was preceded by rapid transient increases in the extractable activities of the enzymes L-phenylalanine ammonia-lyase and chalcone synthase”
Claim · model-written summary
Elicitor treatment selectively induces enzymes associated directly with the phytoalexin pathway in bean cells.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Treatment
- elicitore0
- Outcome
- induction of enzymes associated directly with the phytoalexin pathwaye2
- Direction
- increaseinferrede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of cell suspension cultures of Phaseolus vulgaris c.v. Immuna with an elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum resulted in rapid accumulation of the prenylated 5-hydroxyisoflavanone phytoalexin kievitone followed by later accumulation of the pterocarpan-derived phytoalexin phaseollin”
e2“The results suggest a highly selective induction of enzymes associated directly with the phytoalexin pathway”
Claim · model-written summary
Elicitor application prevented the increase in glutamate dehydrogenase activity seen in control cultures of Phaseolus vulgaris.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Treatment
- elicitor applicatione4
- Outcome
- glutamate dehydrogenase activitye4
- Direction
- lowerinferrede4
- Comparator
- control culturese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of cell suspension cultures of Phaseolus vulgaris c.v. Immuna with an elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum resulted in rapid accumulation of the prenylated 5-hydroxyisoflavanone phytoalexin kievitone followed by later accumulation of the pterocarpan-derived phytoalexin phaseollin”
e4“The increase in glutamate dehydrogenase activity in control cultures was prevented by elicitor application”
Claim · model-written summary
Peroxidase, glutamate dehydrogenase and glutamate synthase activities were higher in Colletotrichum-infected bean hypocotyls than in healthy hypocotyls.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Treatment
- Colletotrichum-infectede6
- Interactor
- ColletotrichumColletotrichume6pathogen
- Outcome
- peroxidase, glutamate dehydrogenase and glutamate synthase activitiese5
- Direction
- highere5
- Comparator
- healthy hypocotylse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of cell suspension cultures of Phaseolus vulgaris c.v. Immuna with an elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum resulted in rapid accumulation of the prenylated 5-hydroxyisoflavanone phytoalexin kievitone followed by later accumulation of the pterocarpan-derived phytoalexin phaseollin”
e5“peroxidase, glutamate dehydrogenase and glutamate synthase activities were higher in infected than in healthy hypocotyls”
e6“A comparison of enzyme activities in control and Colletotrichum-infected bean hypocotyls”
Claim · model-written summary
The major enzymic changes in elicitor-treated bean cells are probably associated with defence mechanisms.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Outcome
- major enzymic changese7
- Qualifier
- defence mechanismse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of cell suspension cultures of Phaseolus vulgaris c.v. Immuna with an elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum resulted in rapid accumulation of the prenylated 5-hydroxyisoflavanone phytoalexin kievitone followed by later accumulation of the pterocarpan-derived phytoalexin phaseollin”
e7“It is concluded that the major enzymic changes occurring in elicitor-treated bean cells are probably those directly associated with defence mechanisms”
The same questions in other papers
Colletotrichum lindemuthianum · molecular: metabolites · Phaseolus vulgaris (common bean)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
heat · molecular: metabolites · Phaseolus vulgaris (common bean)Positive and negative results
7 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- no effect
7 papers more in this group
See all 11 papers →resistance inducer · molecular: metabolites · Phaseolus vulgaris (common bean)Positive and negative results
10 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
9 papers more in this group
See all 13 papers →resistance inducer · physiology: enzyme activity · Phaseolus vulgaris (common bean)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.