Abstract
Chalcone isomerase, an enzyme involved in the formation of flavonoid-derived compounds in plants, has been purified nearly 600-fold from cell suspension cultures of dwarf French bean (Phaseolus vulgaris L.). Chromatofocussing yielded a single form of the enzyme of apparent pI 5.0. This preparation was used to raise rabbit anti-(chalcone isomerase) serum. Changes in the rate of synthesis of chalcone isomerase have been investigated by indirect immunoprecipitation of enzyme labelled in vivo with [35S]methionine in elicitor-treated cultures of P. vulgaris. Elicitor, heat-released from cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum, the causal agent of anthracnose disease of bean, causes increased synthesis of the isomerase, with maximum synthetic rate occurring 11-12 h after exposure to elicitor. Immune blotting studies indicate that the elicitor-mediated increase in extractable activity of the isomerase is associated with increased appearance of immunodetactable isomerase protein of Mr 27 000. However, the maximum level of immunodetectable isomerase was attained approximately 6 h earlier than maximum extractable activity. Furthermore, a 2.8-fold increase in enzyme activity above basal levels at 12 h after elicitor-treatment was associated with a corresponding 5.8-fold increase in immunodetectable enzyme. It is concluded that elicitor induces the synthesis of both active and inactive chalcone isomerase of Mr 27 000, and that some activation of inactive enzyme occurs during the elicitor-mediated increase in isomerase activity. The presence of a pool of inactive chalcone isomerase in bean cell cultures has recently been suggested on the basis of density labelling experiments utilising 2H from 2H2O [Dixon et al. (1983) Planta (Berl.) 159, 561-569].
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Claims 9
Claim · model-written summary
Chalcone isomerase was purified nearly 600-fold from dwarf French bean cell cultures.
- Taxon
- dwarf French bean
Phaseolus vulgarise0 - Outcome
- Chalcone isomerasee0
- Value
- nearly 600-folde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chalcone isomerase, an enzyme involved in the formation of flavonoid-derived compounds in plants, has been purified nearly 600-fold from cell suspension cultures of dwarf French bean (Phaseolus vulgaris L.)”
Claim · model-written summary
Chromatofocussing yielded one enzyme form with apparent pI 5.0.
Verbatim
e0“Chromatofocussing yielded a single form of the enzyme of apparent pI 5.0”
t0“bean cells”
Claim · model-written summary
Elicitor increased isomerase synthesis, with its maximum rate 11-12 h after exposure.
- Taxon
- beane0
- Treatment
- Elicitore0
- Interactor
- Colletotrichum lindemuthianum
Colletotrichum lindemuthianume0pathogen - Gene
- isomerasee0
- Outcome
- synthesis of the isomerasee0
- Direction
- increasede0
- Value
- 11-12 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elicitor, heat-released from cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum, the causal agent of anthracnose disease of bean, causes increased synthesis of the isomerase, with maximum synthetic rate occurring 11-12 h after exposure to elicitor”
Claim · model-written summary
The elicitor-associated increase in extractable isomerase activity accompanied increased immunodetectable protein of Mr 27 000.
Verbatim
e0“the elicitor-mediated increase in extractable activity of the isomerase is associated with increased appearance of immunodetactable isomerase protein of Mr 27 000”
t0“bean cells”
Claim · model-written summary
Maximum immunodetectable isomerase occurred approximately 6 h before maximum extractable activity.
Verbatim
e0“the maximum level of immunodetectable isomerase was attained approximately 6 h earlier than maximum extractable activity”
t0“bean cells”
Claim · model-written summary
At 12 h after elicitor treatment, enzyme activity was 2.8-fold above basal levels.
Verbatim
e0“a 2.8-fold increase in enzyme activity above basal levels at 12 h after elicitor-treatment”
t0“bean cells”
Claim · model-written summary
At 12 h after elicitor treatment, immunodetectable enzyme increased 5.8-fold.
- Taxon
- beant0
- Treatment
- elicitor-treatmente0
- Outcome
- immunodetectable enzymee0
- Direction
- increasee0
- Value
- 5.8-folde0
Not reportedComparator
Verbatim
e0“at 12 h after elicitor-treatment was associated with a corresponding 5.8-fold increase in immunodetectable enzyme”
t0“bean cells”
Claim · model-written summary
The authors conclude that elicitor induces active and inactive chalcone isomerase.
Verbatim
e0“elicitor induces the synthesis of both active and inactive chalcone isomerase of Mr 27 000”
t0“bean cells”
Claim · model-written summary
The authors infer activation of inactive enzyme during the elicitor-mediated activity increase.
Verbatim
e0“some activation of inactive enzyme occurs during the elicitor-mediated increase in isomerase activity”
t0“bean cells”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.