Abstract
BRASSINAZOLE RESISTANT 1 (BZR1), the critical regulator of brassinosteroid (BR) response, participates in various BR-mediated developmental processes. However, the roles of BZR1 in stress tolerance are less clear. Here, we found that BZR1-like protein in tomato controls BR response and is involved in thermotolerance by regulating the FERONIA (FER) homologs. The CRISPR-bzr1 mutant showed reduced growth and was not responsive to 24-epibrassinolide (EBR) with regard to the promotion of plant growth. Mutation in BZR1 impaired the induction of RESPIRATORY BURST OXIDASE HOMOLOG1 (RBOH1), production of H2O2 in the apoplast and heat tolerance. Exogenous H2O2 recovered the heat tolerance of the tomato bzr1 mutant. Overexpression of BZR1 enhanced the production of apoplastic H2O2 and heat stress responses. However, silencing of RBOH1 abolished the BZR1-mediated heat tolerance. Further analysis showed that BZR1 bound to the promoters of FERONIA2 (FER2) and FER3 and induced their expression. Silencing of FER2/3 suppressed BZR1-dependent BR signaling for the induction of RBOH1 transcripts, accumulation of apoplastic H2O2 and heat tolerance. These results indicate that BZR1 regulates heat stress responses in tomato through RBOH1-dependent reactive oxygen species (ROS) signaling, which is at least partially mediated by FER2 and FER3.
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Claims 14
Claim · model-written summary
CRISPR-bzr1 mutation reduced growth.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- CRISPR-bzr1 mutante0
- Outcome
- growthe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The CRISPR-bzr1 mutant showed reduced growth”
t0“tomato”
Claim · model-written summary
CRISPR-bzr1 plants did not respond to EBR growth promotion.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- 24-epibrassinolide (EBR)e0
- Outcome
- promotion of plant growthe0
- Direction
- not responsivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was not responsive to 24-epibrassinolide (EBR) with regard to the promotion of plant growth”
t0“tomato”
Claim · model-written summary
BZR1 mutation impaired RBOH1 induction.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Mutation in BZR1e0
- Outcome
- induction of RESPIRATORY BURST OXIDASE HOMOLOG1 (RBOH1)e0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation in BZR1 impaired the induction of RESPIRATORY BURST OXIDASE HOMOLOG1 (RBOH1), production of H2O2 in the apoplast and heat tolerance.”
t0“tomato”
Claim · model-written summary
BZR1 mutation impaired apoplastic H2O2 production.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Mutation in BZR1e0
- Outcome
- production of H2O2 in the apoplaste0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation in BZR1 impaired the induction of RESPIRATORY BURST OXIDASE HOMOLOG1 (RBOH1), production of H2O2 in the apoplast and heat tolerance.”
t0“tomato”
Claim · model-written summary
BZR1 mutation impaired heat tolerance.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Mutation in BZR1e0
- Outcome
- heat tolerancee0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation in BZR1 impaired the induction of RESPIRATORY BURST OXIDASE HOMOLOG1 (RBOH1), production of H2O2 in the apoplast and heat tolerance.”
t0“tomato”
Claim · model-written summary
Exogenous H2O2 restored heat tolerance in bzr1 tomato.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Exogenous H2O2e0
- Outcome
- heat tolerancee0
- Direction
- recoverede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous H2O2 recovered the heat tolerance of the tomato bzr1 mutant.”
t0“tomato”
Claim · model-written summary
BZR1 overexpression enhanced apoplastic H2O2 production.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Overexpression of BZR1e0
- Outcome
- production of apoplastic H2O2e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of BZR1 enhanced the production of apoplastic H2O2 and heat stress responses.”
t0“tomato”
Claim · model-written summary
BZR1 overexpression enhanced heat stress responses.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Overexpression of BZR1e0
- Outcome
- heat stress responsese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of BZR1 enhanced the production of apoplastic H2O2 and heat stress responses.”
t0“tomato”
Claim · model-written summary
RBOH1 silencing abolished BZR1-mediated heat tolerance.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- silencing of RBOH1e0
- Outcome
- BZR1-mediated heat tolerancee0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of RBOH1 abolished the BZR1-mediated heat tolerance”
t0“tomato”
Claim · model-written summary
BZR1 bound FER2 and FER3 promoters.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- BZR1e0
- Outcome
- promoters of FERONIA2 (FER2) and FER3e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BZR1 bound to the promoters of FERONIA2 (FER2) and FER3 and induced their expression.”
t0“tomato”
Claim · model-written summary
BZR1 induced FER2 and FER3 expression.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- BZR1e0
- Outcome
- their expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BZR1 bound to the promoters of FERONIA2 (FER2) and FER3 and induced their expression.”
t0“tomato”
Claim · model-written summary
FER2/3 silencing suppressed RBOH1 transcript induction.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Silencing of FER2/3e0
- Outcome
- induction of RBOH1 transcriptse0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of FER2/3 suppressed BZR1-dependent BR signaling for the induction of RBOH1 transcripts, accumulation of apoplastic H2O2 and heat tolerance.”
t0“tomato”
Claim · model-written summary
FER2/3 silencing suppressed apoplastic H2O2 accumulation.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Silencing of FER2/3e0
- Outcome
- accumulation of apoplastic H2O2e0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of FER2/3 suppressed BZR1-dependent BR signaling for the induction of RBOH1 transcripts, accumulation of apoplastic H2O2 and heat tolerance.”
t0“tomato”
Claim · model-written summary
FER2/3 silencing suppressed heat tolerance.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Silencing of FER2/3e0
- Outcome
- heat tolerancee0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of FER2/3 suppressed BZR1-dependent BR signaling for the induction of RBOH1 transcripts, accumulation of apoplastic H2O2 and heat tolerance.”
t0“tomato”
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