Abstract
A complete cDNA of potato Phytophthora infestans-induced hypersensitive response-related protein gene ( POTHR- f) was cloned using rapid amplification of cDNA ends (RACE) strategy according to a fragment sequence which we had cloned using suppression subtractive hybridization (SSH) technique. The potato POTHR-1 gene encodes a protein of 225 amino acids, which shares 81% identity with tobacco hin1 gene-enoded protein (harpin-induced protein). Southern blot revealed that there are two to three copies of POTHR-1 in potato genome. The POTHR-1 gene expression in potato leaves showed that its transcripts accumulated remarkably in leaves after 36 h inoculation with P. infestans. Mechanical wounding and jasmonic acid (JA) could induce the POTHR-1 gene expression and osmotic stress just induce a slight accumulation of POTHR-1 gene mRNA, while salicylic acid (SA) had no detectable function on the induction accumulation of POTHR-1 gene transcripts. The potato POTHR-1 gene may preferentially associate with hypersensitive response (HR) or biotic cell death during interaction between host and pathogen.
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Claims 10
Claim · model-written summary
A complete potato POTHR cDNA was cloned.
- Taxon
- potato
Solanum tuberosume0 - Gene
- POTHR- fe0
- Outcome
- complete cDNAe0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A complete cDNA of potato Phytophthora infestans-induced hypersensitive response-related protein gene ( POTHR- f) was cloned”
Claim · model-written summary
Potato POTHR-1 encodes a 225-amino-acid protein.
- Taxon
- potatoSolanum tuberosume0
- Gene
- POTHR-1e0
- Outcome
- proteine0
- Value
- 225 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The potato POTHR-1 gene encodes a protein of 225 amino acids”
Claim · model-written summary
The POTHR-1 protein shares 81% identity with the tobacco hin1 encoded protein.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- hin1e0
- Outcome
- identitye0
- Value
- 81%e0
- Comparator
- tobacco hin1 gene-enoded proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shares 81% identity with tobacco hin1 gene-enoded protein”
t0“Potato”
Claim · model-written summary
The potato genome contains two to three POTHR-1 copies.
- Taxon
- potatoSolanum tuberosume0
- Gene
- POTHR-1e0
- Outcome
- copies of POTHR-1e0
- Value
- two to three copiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there are two to three copies of POTHR-1 in potato genome”
Claim · model-written summary
POTHR-1 transcripts accumulated markedly in potato leaves 36 h after P. infestans inoculation.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- inoculation with P. infestanse0
- Interactor
- P. infestans
Phytophthora infestanse0pathogen - Gene
- POTHR-1t0
- Outcome
- transcriptse0
- Direction
- accumulated remarkablye0
- Qualifier
- 36 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcripts accumulated remarkably in leaves after 36 h inoculation with P. infestans”
t0“Potato POTHR-1”
Claim · model-written summary
Mechanical wounding induced POTHR-1 expression.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Mechanical woundinge0
- Gene
- POTHR-1e0
- Outcome
- POTHR-1 gene expressione0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mechanical wounding and jasmonic acid (JA) could induce the POTHR-1 gene expression”
t0“Potato”
Claim · model-written summary
Jasmonic acid induced POTHR-1 expression.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- jasmonic acid (JA)e0
- Gene
- POTHR-1e0
- Outcome
- POTHR-1 gene expressione0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“jasmonic acid (JA) could induce the POTHR-1 gene expression”
t0“Potato”
Claim · model-written summary
Osmotic stress induced only slight POTHR-1 mRNA accumulation.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- osmotic stresse0
- Gene
- POTHR-1e0
- Outcome
- POTHR-1 gene mRNAe0
- Direction
- accumulatione0
- Qualifier
- slighte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“osmotic stress just induce a slight accumulation of POTHR-1 gene mRNA”
t0“Potato”
Claim · model-written summary
Salicylic acid had no detectable induction of POTHR-1 transcripts.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- salicylic acid (SA)e0
- Gene
- POTHR-1e0
- Outcome
- POTHR-1 gene transcriptse0
- Direction
- no detectablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salicylic acid (SA) had no detectable function on the induction accumulation of POTHR-1 gene transcripts”
t0“Potato”
Claim · model-written summary
POTHR-1 may preferentially associate with hypersensitive response or biotic cell death in potato pathogen interaction.
- Taxon
- potatoSolanum tuberosume0
- Gene
- POTHR-1e0
- Outcome
- hypersensitive response (HR) or biotic cell deathe0
- Qualifier
- may preferentiallye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The potato POTHR-1 gene may preferentially associate with hypersensitive response (HR) or biotic cell death during interaction between host and pathogen”
The same questions in other papers
salicylic acid · molecular: expression · Solanum tuberosum (potato)Positive and negative results
15 in the opposite direction
- positive
- positive
- positive
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.