Abstract
A collection of cDNAs was isolated from a λgt11 potato ( Solanum tuberosum L.) tuber library by using a soybean lipoxygenase (LOX; linoleate:oxygen oxidoreductase, EC 1.13.11.12) sequence as a hybridization probe. These cDNA clones were classified by restriction patterns and sizes and two clones, plox 1 and plox 2, were selected for sequencing and further analysis. These clones were found to be virtually identical and contain full‐length cDNAs having a high degree of homology with other cloned LOX genes. plox 1 contains a 2 806‐bp insert, with an open reading frame that encodes a protein of 861 amino acids (96.9 kDa), consistent with molecular mass estimates of the principal tuber LOX deduced from SDS‐PAGE. The deduced sequence contains iron‐binding domains that are conserved among LOXs. Restriction analysis of potato genomic DNA confirmed that potato LOX is comprised of a gene family with at least three members. The plox 1 sequence hybridized to a transcript of 2.8 kb in gel blot analyses of total RNA extracted from potato tuber. Treatment of potato disks and leaves with methyl jasmonate or jasmonic acid increased LOX enzyme activity, as well as abundance of LOX mRNA, consistent with jasmonate's reported effect on LOX expression in plants. The fungal elicitor arachidonic acid (AA) strongly induced LOX enzyme activity in leaves and induced LOX mRNA transcripts in potato leaves and tuber disks. Infection of potato leaves with Phytophthora infestans induced LOX activity as well as LOX mRNA accumulation in both incompatible and compatible interaction types. LOX expression was compared with that of a wound‐inducible proteinase inhibitor (PINII) and a pathogenesis‐related protein (P4). Jasmonates induced PINII but not P4 transcript accumulation in potato leaves. In contrast, AA induced P4 but not PINII transcript accumulation in potato leaves. Infection with P. infestans resulted in a gene expression pattern similar to that observed with AA.
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Claims 15
Claim · model-written summary
The plox 1 and plox 2 cDNA clones were virtually identical and full length, with high homology to other LOX genes.
- Taxon
- potato
Solanum tuberosumt0 - Gene
- plox 1e1
- Outcome
- virtually identical and contain full‐length cDNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These clones were found to be virtually identical and contain full‐length cDNAs having a high degree of homology with other cloned LOX genes.”
t0“potato tuber lipoxygenase cDNAs and lipoxygenase expression in potato tubers and leaves”
e1“two clones, plox 1 and plox 2”
Claim · model-written summary
plox 1 contains a 2 806-bp insert and encodes an 861-amino-acid, 96.9-kDa protein.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- plox 1e0
- Outcome
- open reading frame that encodes a proteine0
- Value
- 861 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plox 1 contains a 2 806‐bp insert, with an open reading frame that encodes a protein of 861 amino acids (96.9 kDa)”
t0“potato tuber lipoxygenase cDNAs”
Claim · model-written summary
The deduced LOX sequence contains conserved iron-binding domains.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- LOXse0
- Outcome
- iron‐binding domainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deduced sequence contains iron‐binding domains that are conserved among LOXs.”
t0“potato tuber lipoxygenase cDNAs”
Claim · model-written summary
Potato LOX comprises a gene family with at least three members.
- Taxon
- potatoSolanum tuberosume0
- Gene
- LOXe0
- Outcome
- gene familye0
- Value
- at least three memberse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potato LOX is comprised of a gene family with at least three members.”
Claim · model-written summary
plox 1 hybridized to a 2.8 kb transcript in potato tuber RNA.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plox 1 sequence hybridized to a transcript of 2.8 kb in gel blot analyses of total RNA extracted from potato tuber.”
Claim · model-written summary
Methyl jasmonate increased LOX activity and LOX mRNA abundance in potato disks and leaves.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- disks and leavese0
- Treatment
- methyl jasmonatee0
- Outcome
- LOX enzyme activity, as well as abundance of LOX mRNAe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of potato disks and leaves with methyl jasmonate or jasmonic acid increased LOX enzyme activity, as well as abundance of LOX mRNA”
Claim · model-written summary
Jasmonic acid increased LOX activity and LOX mRNA abundance in potato disks and leaves.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- disks and leavese0
- Treatment
- jasmonic acide0
- Outcome
- LOX enzyme activity, as well as abundance of LOX mRNAe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of potato disks and leaves with methyl jasmonate or jasmonic acid increased LOX enzyme activity, as well as abundance of LOX mRNA”
Claim · model-written summary
Arachidonic acid strongly induced LOX activity in potato leaves.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- leavese0
- Treatment
- arachidonic acide0
- Outcome
- LOX enzyme activitye0
- Direction
- strongly inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fungal elicitor arachidonic acid (AA) strongly induced LOX enzyme activity in leaves and induced LOX mRNA transcripts in potato leaves and tuber disks.”
Claim · model-written summary
Arachidonic acid induced LOX mRNA in potato leaves and tuber disks.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- leaves and tuber diskse0
- Treatment
- arachidonic acide0
- Outcome
- LOX mRNA transcriptse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fungal elicitor arachidonic acid (AA) strongly induced LOX enzyme activity in leaves and induced LOX mRNA transcripts in potato leaves and tuber disks.”
Claim · model-written summary
Phytophthora infestans infection induced potato leaf LOX activity and mRNA in both interaction types.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- Infection of potato leaves with Phytophthora infestanse0
- Interactor
- Phytophthora infestans
Phytophthora infestanse0pathogen - Outcome
- LOX activity as well as LOX mRNA accumulatione0
- Direction
- inducede0
- Qualifier
- both incompatible and compatible interaction typese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infection of potato leaves with Phytophthora infestans induced LOX activity as well as LOX mRNA accumulation in both incompatible and compatible interaction types.”
Claim · model-written summary
Jasmonates induced PINII but not P4 transcript accumulation in potato leaves.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jasmonates induced PINII but not P4 transcript accumulation in potato leaves.”
Claim · model-written summary
Jasmonates did not induce P4 transcripts in potato leaves.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- Jasmonatese0
- Gene
- P4e0
- Outcome
- P4 transcript accumulatione0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jasmonates induced PINII but not P4 transcript accumulation in potato leaves.”
Claim · model-written summary
Arachidonic acid induced P4 transcripts in potato leaves.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AA induced P4 but not PINII transcript accumulation in potato leaves.”
Claim · model-written summary
Arachidonic acid did not induce PINII transcripts in potato leaves.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- AAe0
- Gene
- PINIIe0
- Outcome
- PINII transcript accumulatione0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AA induced P4 but not PINII transcript accumulation in potato leaves.”
Claim · model-written summary
P. infestans infection produced a gene expression pattern similar to arachidonic acid treatment.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Outcome
- gene expression pattern similar to that observed with AAe0
- Comparator
- AAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infection with P. infestans resulted in a gene expression pattern similar to that observed with AA.”
t0“potato tuber lipoxygenase cDNAs”
The same questions in other papers
(-)-methyl jasmonate · molecular: expression · Solanum tuberosum (potato)Positive and negative results
6 papers in the same direction as this paper · 2 in the opposite direction
- positive
- no effect
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See all 9 papers →jasmonic acid · molecular: expression · Solanum tuberosum (potato)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.