Abstract
The present research was aiming to study In-Silico the effect of Glycyrrhizic Acid ammonium salt (GAS) and Salicylic acid (SA) on the coat protein of potato leafroll virus (PLRV). In addition, in-vitro studying the effect of (GAS NPs) and Salicylic acid (SA NPs) nanoparticles at concentrations 0.15, 0.30, 1.25 and 2.5 mM, respectively, to control, decline or reduce the presence of PLRV in potato plants Solanum tuberosum L. selena. (GAS NPs) and (SA NPs) were applied in the MS medium at concentrations 0.15, 0.30, 1.25 and 2.5 mM, respectively. Results revealed that, enhancement or decline the PLRV according to the initiation of specific pathways. The expression level of Kinase 3 gene increased significantly due to the two used concentrations of GAS NPs. While the expression of callose gene was upregulated significantly in response to treatment of PLRV infected plant with (GAS NPs) with concentration (0.30 mM). Treatment with (SA NPs) caused upregulation significance only of callose gene at (2.5 mM) concentration. The molecular modeling results of used compounds (glycyrrhizic acid ammonium salt and salicylic acid) showed highest score of binding and the best rms define value with a very good binding mode and perfect interactions with amino acids of the three subunits (A, B and C) forming the protein coat of leaf roll virus. Glycyrrhizic acid ammonium salt and salicylic acid nanoparticles could be perfect solution to produce potato plant free virus in-vitro. Further larger studies are needed to investigate the role of the studied compounds in vivo.
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Claims 7
Claim · model-written summary
GAS nanoparticles significantly increased Kinase 3 expression at two concentrations.
- Taxon
- potato plants Solanum tuberosum L. selena
Solanum tuberosume1 - Treatment
- GAS NPse0
- Gene
- Kinase 3 genee0
- Outcome
- expression level of Kinase 3 genee0
- Direction
- increasede0
- Qualifier
- two used concentrationse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression level of Kinase 3 gene increased significantly due to the two used concentrations of GAS NPs”
e1“potato plants Solanum tuberosum L. selena”
Claim · model-written summary
0.30 mM GAS nanoparticles significantly upregulated callose expression in PLRV-infected potato.
- Taxon
- potato plants Solanum tuberosum L. selenaSolanum tuberosume1
- Treatment
- GAS NPse0
- Interactor
- potato leafroll virus (PLRV)
Potato leafroll viruse2pathogen - Gene
- callose genee0
- Outcome
- expression of callose genee0
- Direction
- upregulatede0
- Value
- 0.30 mMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of callose gene was upregulated significantly in response to treatment of PLRV infected plant with (GAS NPs) with concentration (0.30 mM)”
e1“potato plants Solanum tuberosum L. selena”
e2“potato leafroll virus (PLRV)”
Claim · model-written summary
2.5 mM SA nanoparticles significantly upregulated callose expression.
- Taxon
- potato plants Solanum tuberosum L. selenaSolanum tuberosume1
- Treatment
- SA NPse0
- Gene
- callose genee0
- Outcome
- callose genee0
- Direction
- upregulatione0
- Value
- 2.5 mMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment with (SA NPs) caused upregulation significance only of callose gene at (2.5 mM) concentration”
e1“potato plants Solanum tuberosum L. selena”
Claim · model-written summary
Glycyrrhizic acid ammonium salt showed a high binding score and favorable modeled interaction with PLRV coat protein.
- Taxon
- potato plants Solanum tuberosum L. selenaSolanum tuberosume1
- Interactor
- leaf roll viruse0pathogen
- Compound
- glycyrrhizic acid ammonium salte0
- Outcome
- highest score of bindinge0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glycyrrhizic acid ammonium salt and salicylic acid) showed highest score of binding and the best rms define value with a very good binding mode and perfect interactions with amino acids of the three subunits (A, B and C) forming the protein coat of leaf roll virus”
e1“potato plants Solanum tuberosum L. selena”
Claim · model-written summary
Salicylic acid showed a high binding score and favorable modeled interaction with PLRV coat protein.
- Taxon
- potato plants Solanum tuberosum L. selenaSolanum tuberosume1
- Interactor
- leaf roll viruse0pathogen
- Compound
- salicylic acide0
- Outcome
- highest score of bindinge0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glycyrrhizic acid ammonium salt and salicylic acid) showed highest score of binding and the best rms define value with a very good binding mode and perfect interactions with amino acids of the three subunits (A, B and C) forming the protein coat of leaf roll virus”
e1“potato plants Solanum tuberosum L. selena”
Claim · model-written summary
Authors suggest GAS and SA nanoparticles could produce virus-free potato plants in vitro.
- Taxon
- potato plantSolanum tuberosume0
- Outcome
- produce potato plant free virus in-vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glycyrrhizic acid ammonium salt and salicylic acid nanoparticles could be perfect solution to produce potato plant free virus in-vitro”
Claim · model-written summary
Authors call for larger in vivo studies of the compounds.
- Taxon
- potato plants Solanum tuberosum L. selenaSolanum tuberosume1
- Outcome
- larger studies are needed to investigate the role of the studied compounds in vivoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Further larger studies are needed to investigate the role of the studied compounds in vivo”
e1“potato plants Solanum tuberosum L. selena”
The same questions in other papers
salicylic acid · molecular: expression · Solanum tuberosum (potato)Positive and negative results
14 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
12 papers more in this group
See all 16 papers →nanoparticle · molecular: expression · Solanum tuberosum (potato)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.