Abstract
The present investigation has been concentrated on the establishment of an efficacious method for in vitro regeneration of a Musa cultivar Gopi of Tripura. Focus has been greatly anchored to the sterilization procedure for assuring successful aseptic culture establishment of this cultivar. The culture protocol for shoot induction and proliferation comprised of four different treatments in basal MS medium fortified with 6-Benzylaminopurine of varying concentrations. The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l-1 for shoot bud induction and multiplication. Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l-1). Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid. The best rooting was manifested in treatment with IBA at concentration of 2mg l-1. The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India.
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Claims 4
Claim · model-written summary
BAP at 8 mg/l in MS medium gave the optimum response for shoot bud induction and multiplication in Musa cultivar Gopi.
- Material
- Gopie1
- Organ / stage
- shoot bude0
- Treatment
- BAP in MS medium at concentration of 8mg l-1e0
- Outcome
- shoot bud induction and multiplicatione0
- Direction
- optimum responsee0
MusaNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l-1 for shoot bud induction and multiplication”
e1“in vitro regeneration of a Musa cultivar Gopi of Tripura”
Claim · model-written summary
Shoot growth and multiplication of Musa cultivar Gopi was achieved through repeated subcultures on medium with 4 mg/l BAP.
- Material
- Gopie1
- Treatment
- repeated subcultures in media containing lower concentration (4 mg l-1)e2
- Outcome
- shoot growth and multiplicatione2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in vitro regeneration of a Musa cultivar Gopi of Tripura”
e2“Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l-1)”
Claim · model-written summary
Root initiation in Musa cultivar Gopi was stimulated by Indole butyric acid at two concentrations.
- Material
- Gopie1
- Organ / stage
- Roote3
- Treatment
- Indole butyric acide3
- Outcome
- Root initiatione3
- Direction
- stimulatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in vitro regeneration of a Musa cultivar Gopi of Tripura”
e3“Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid”
Claim · model-written summary
IBA at 2 mg/l gave the best rooting of Musa cultivar Gopi.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in vitro regeneration of a Musa cultivar Gopi of Tripura”
e4“The best rooting was manifested in treatment with IBA at concentration of 2mg l-1”
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