Abstract
An investigation has been conducted on Fast Protocol for in vitro cloning of Banana (Musa acuminata) cv. Amritpani at the Biotechnology-cum-Tissue Culture Center, OUAT, Bhubaneswar, during the year 2014-16. This has helped to determine the best media compositions for callus formation, shoot initiation, shoot proliferation and rooting of banana cv. Amritpani, so as to get optimum results with a minimized cost of productions. MS medium supplemented by 7.0 mg/l + 0.5 mg/l IAA, significantly enhance the callus production, MS medium fortified by 5.0 mg/l + 0.5 mg/l IAA was found to be most potent combination and produced a significantly higher number of multiple shoots per clump (29.77), after 30 days of culturing. The same combination recorded significantly higher number of shoots/explants. i.e., 30.03 and 12.33 in 1st and 2nd sub-culturing respectively. MS medium fortified with 1.0 mg/l IBA was found to be ideal and produced maximum number of roots/plants (5.08) and average root length (5.30 cm). This protocol might be used for the massive in vitro production of plantlets of banana cv. Amritpani.
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Claims 6
Claim · model-written summary
The stated MS medium significantly enhanced callus production.
- Taxon
- Bananat0
- Treatment
- MS medium supplemented by 7.0 mg/l + 0.5 mg/l IAAe0
- Outcome
- callus productione0
- Direction
- enhancee0
Not reportedComparator
Verbatim
e0“MS medium supplemented by 7.0 mg/l + 0.5 mg/l IAA, significantly enhance the callus production”
t0“Banana”
Claim · model-written summary
The 5.0 mg/l plus IAA medium produced significantly more multiple shoots per clump after 30 days.
- Taxon
- Bananat0
- Treatment
- MS medium fortified by 5.0 mg/l + 0.5 mg/l IAAe0
- Outcome
- number of multiple shoots per clumpe0
- Direction
- highere0
- Value
- 29.77e0
- Qualifier
- after 30 days of culturinge0
Not reportedComparator
Verbatim
e0“MS medium fortified by 5.0 mg/l + 0.5 mg/l IAA was found to be most potent combination and produced a significantly higher number of multiple shoots per clump (29.77), after 30 days of culturing.”
t0“Banana”
Claim · model-written summary
The same combination yielded 30.03 shoots per explant in the first subculture.
- Taxon
- Bananat0
- Treatment
- same combinatione0
- Outcome
- number of shoots/explantse0
- Direction
- highere0
- Value
- 30.03e0
- Qualifier
- 1ste0
Not reportedComparator
Verbatim
e0“The same combination recorded significantly higher number of shoots/explants. i.e., 30.03 and 12.33 in 1st and 2nd sub-culturing respectively.”
t0“Banana”
Claim · model-written summary
The same combination yielded 12.33 shoots per explant in the second subculture.
- Taxon
- Bananat0
- Treatment
- same combinatione0
- Outcome
- number of shoots/explantse0
- Direction
- highere0
- Value
- 12.33e0
- Qualifier
- 2nde0
Not reportedComparator
Verbatim
e0“The same combination recorded significantly higher number of shoots/explants. i.e., 30.03 and 12.33 in 1st and 2nd sub-culturing respectively.”
t0“Banana”
Claim · model-written summary
The IBA medium produced a maximum of 5.08 roots per plant.
- Taxon
- Bananat0
- Treatment
- MS medium fortified with 1.0 mg/l IBAe0
- Outcome
- number of roots/plantse0
- Direction
- maximume0
- Value
- 5.08e0
Not reportedComparator
Verbatim
e0“MS medium fortified with 1.0 mg/l IBA was found to be ideal and produced maximum number of roots/plants (5.08) and average root length (5.30 cm).”
t0“Banana”
Claim · model-written summary
The IBA medium produced an average root length of 5.30 cm.
- Taxon
- Bananat0
- Treatment
- MS medium fortified with 1.0 mg/l IBAe0
- Outcome
- average root lengthe0
- Value
- 5.30 cme0
Not reportedComparatorDirection
Verbatim
e0“MS medium fortified with 1.0 mg/l IBA was found to be ideal and produced maximum number of roots/plants (5.08) and average root length (5.30 cm).”
t0“Banana”
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