Abstract
Banana micropropagation is commercially established by the use of shoot tips obtained from intact plant on shoot multiplication medium under optimal culture conditions. However, this is somewhat associated with tissue browning, which may be associated with a reduced efficiency of shoot multiplication process. An increase or decrease in incubation temperature from 27 ºC, change the texture of the media from semisolid state, addition of ascorbic acid and substitution of optimal concentration of benzyl amino purine (5 mg/l BAP) with 2-isopentyl adenine (4 mg/l 2ip), decreased shoot multiplication and growth, even if they reduced tissue browning. Our results indicated that the chemical composition of the MS medium containing 5 mg/l BAP was ideal for banana shoot multiplication. Addition of some natural ingredients rich in many important components (yeast extract, coconut endosperm milk or moringa leaf extract) was unsuitable for in vitro multiplication of banana. The observed exception was that growth parameters of shoots formed on MS medium with 4 mg/l 2ip were better than those formed on MS with 5 mg/l BAP. In addition, shoot multiplication on 2-isopentenyl adenine (2ip) containing media showed root formation, which facilitated the adaptation and transfer of plants to soil.
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Claims 11
Claim · model-written summary
Incubation temperatures above or below 27 ºC reduced banana shoot multiplication and growth.
Verbatim
t0“Banana cv. Grand Naine”
e0“An increase or decrease in incubation temperature from 27 ºC, change the texture of the media from semisolid state, addition of ascorbic acid and substitution of optimal concentration of benzyl amino purine (5 mg/l BAP) with 2-isopentyl adenine (4 mg/l 2ip), decreased shoot multiplication and growth, even if they reduced tissue browning”
Claim · model-written summary
Changing the medium from semisolid reduced banana shoot multiplication and growth.
Verbatim
t0“Banana cv. Grand Naine”
e0“change the texture of the media from semisolid state, addition of ascorbic acid and substitution of optimal concentration of benzyl amino purine (5 mg/l BAP) with 2-isopentyl adenine (4 mg/l 2ip), decreased shoot multiplication and growth”
Claim · model-written summary
Adding ascorbic acid reduced banana shoot multiplication and growth.
- Taxon
- Bananat0
- Treatment
- addition of ascorbic acide0
- Outcome
- shoot multiplication and growthe0
- Direction
- decreasede0
Not reportedComparator
Verbatim
t0“Banana cv. Grand Naine”
e0“addition of ascorbic acid and substitution of optimal concentration of benzyl amino purine (5 mg/l BAP) with 2-isopentyl adenine (4 mg/l 2ip), decreased shoot multiplication and growth”
Claim · model-written summary
Replacing 5 mg/l BAP with 4 mg/l 2ip reduced banana shoot multiplication.
Verbatim
t0“Banana cv. Grand Naine”
e0“substitution of optimal concentration of benzyl amino purine (5 mg/l BAP) with 2-isopentyl adenine (4 mg/l 2ip), decreased shoot multiplication and growth”
Claim · model-written summary
The treatments that reduced multiplication and growth also reduced banana tissue browning.
Verbatim
t0“Banana cv. Grand Naine”
e0“decreased shoot multiplication and growth, even if they reduced tissue browning”
Claim · model-written summary
MS medium containing 5 mg/l BAP was judged ideal for banana shoot multiplication.
Verbatim
t0“Banana cv. Grand Naine”
e0“the chemical composition of the MS medium containing 5 mg/l BAP was ideal for banana shoot multiplication”
Claim · model-written summary
Yeast extract was unsuitable for in vitro banana multiplication.
Verbatim
t0“Banana cv. Grand Naine”
e0“Addition of some natural ingredients rich in many important components (yeast extract, coconut endosperm milk or moringa leaf extract) was unsuitable for in vitro multiplication of banana”
Claim · model-written summary
Coconut endosperm milk was unsuitable for in vitro banana multiplication.
Verbatim
e0“Addition of some natural ingredients rich in many important components (yeast extract, coconut endosperm milk or moringa leaf extract) was unsuitable for in vitro multiplication of banana”
Claim · model-written summary
Moringa leaf extract was unsuitable for in vitro banana multiplication.
Verbatim
e0“Addition of some natural ingredients rich in many important components (yeast extract, coconut endosperm milk or moringa leaf extract) was unsuitable for in vitro multiplication of banana”
Claim · model-written summary
Banana shoot growth parameters were better on MS medium with 4 mg/l 2ip than with 5 mg/l BAP.
Verbatim
t0“Banana cv. Grand Naine”
e0“growth parameters of shoots formed on MS medium with 4 mg/l 2ip were better than those formed on MS with 5 mg/l BAP”
Claim · model-written summary
Banana shoot multiplication on 2ip media produced roots that facilitated adaptation and transfer to soil.
Not reportedDirection
Verbatim
t0“Banana cv. Grand Naine”
e0“shoot multiplication on 2-isopentenyl adenine (2ip) containing media showed root formation, which facilitated the adaptation and transfer of plants to soil”
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See all 68 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.