Abstract
Banana is a staple food and income crop worldwide.Here, we identified the best practices for the initiation of banana (Musa spp.) cultures.The cultivars studied included East African highland bananas (Mshare/Mchare and Nshakara) and plantains (Mzuzu), which are highly demanded by farmers.The following factors were assessed: sterilization techniques for rainy and dry seasons, explant size, effect of benzylaminopurine (BAP) concentration on regeneration of cultures, effect of ascorbic acid on culture browning, and culturing conditions.We found that treatment with ethanol alone (70%) or in combination with sodium hypochlorite (1%) yielded a relatively higher proportion (≥ 96%) of aseptic culture establishment in both rainy and dry seasons.Explant size affected the survival and regeneration of cultures where small explants (˂ 10 mm) like meristem dome showed relatively slower re-growth and high mortality in contrast to large explants (≥ 10 mm).Moreover, culturing in BAP-supplemented Murashige and Skoog medium (MS) significantly enhanced the greening/shoot regeneration percentage at 14 d.However, there was no significant increase in number of green shoots/culture regeneration at BAP concentrations of 5 mg/l, 6 mg/l, and 8 mg/l for Nshakara and 3 mg/l, 5 mg/l, and 6 mg/l for Mzuzu.Browning decreased by 18% when explants were pre-treated with 100 mg/l ascorbic acid for 10 min.Further, growth conditions such as light were a relatively greater determinant for regeneration of initiated banana cultures.Therefore, our findings suggest the use of appropriate sterilization techniques, explant size, growth regulators, and conditions to ensure sufficient production of planting materials.
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Claims 8
Claim · model-written summary
Ethanol alone or with sodium hypochlorite gave at least 96% aseptic establishment.
- Treatment
- ethanol alone (70%) or in combination with sodium hypochlorite (1%)e0
- Outcome
- aseptic culture establishmente0
- Direction
- highere0
- Value
- ≥ 96%e0
MusaNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with ethanol alone (70%) or in combination with sodium hypochlorite (1%) yielded a relatively higher proportion (≥ 96%) of aseptic culture establishment in both rainy and dry seasons”
t0“banana and plantain (Musa spp.) cultures”
Claim · model-written summary
Small explants regrew more slowly and had high mortality compared with large explants.
Verbatim
e0“small explants (˂ 10 mm) like meristem dome showed relatively slower re-growth and high mortality in contrast to large explants (≥ 10 mm)”
t0“banana and plantain”
Claim · model-written summary
BAP medium significantly enhanced greening and shoot regeneration at 14 days.
- Taxon
- bananat0
- Treatment
- BAP-supplemented Murashige and Skoog medium (MS)e0
- Outcome
- greening/shoot regeneration percentagee0
- Direction
- enhancede0
- Qualifier
- at 14 de0
Not reportedComparator
Verbatim
e0“BAP-supplemented Murashige and Skoog medium (MS) significantly enhanced the greening/shoot regeneration percentage at 14 d”
t0“banana and plantain”
Claim · model-written summary
Specified BAP concentrations did not significantly increase green shoots in Nshakara.
- Taxon
- bananat0
- Material
- Nshakarae0
- Treatment
- BAP concentrations of 5 mg/l, 6 mg/l, and 8 mg/le0
- Outcome
- number of green shoots/culture regeneratione0
- Direction
- no significant increasee0
Not reportedComparator
Verbatim
e0“no significant increase in number of green shoots/culture regeneration at BAP concentrations of 5 mg/l, 6 mg/l, and 8 mg/l for Nshakara”
t0“banana and plantain”
Claim · model-written summary
Specified BAP concentrations did not significantly increase green shoots in Mzuzu.
- Taxon
- bananat0
- Material
- Mzuzue0
- Treatment
- 3 mg/l, 5 mg/l, and 6 mg/le0
- Outcome
- number of green shoots/culture regeneratione0
- Direction
- no significant increasee0
Not reportedComparator
Verbatim
e0“no significant increase in number of green shoots/culture regeneration at BAP concentrations of 5 mg/l, 6 mg/l, and 8 mg/l for Nshakara and 3 mg/l, 5 mg/l, and 6 mg/l for Mzuzu”
t0“banana and plantain”
Claim · model-written summary
Ascorbic acid pretreatment reduced browning by 18%.
- Taxon
- bananat0
- Treatment
- 100 mg/l ascorbic acid for 10 mine0
- Outcome
- Browninge0
- Direction
- decreasede0
- Value
- 18%e0
Not reportedComparator
Verbatim
e0“Browning decreased by 18% when explants were pre-treated with 100 mg/l ascorbic acid for 10 min”
t0“banana and plantain”
Claim · model-written summary
Light was a relatively greater determinant of culture regeneration.
Verbatim
e0“growth conditions such as light were a relatively greater determinant for regeneration of initiated banana cultures”
Claim · model-written summary
Authors recommend suitable sterilization, explant size, regulators and conditions for planting material production.
Verbatim
e0“use of appropriate sterilization techniques, explant size, growth regulators, and conditions to ensure sufficient production of planting materials”
t0“banana and plantain”
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