Abstract
The present study was to investigate the best plant growth regulators for shoot proliferation and multiplication; and to determine the optimum concentrations of phytohormones for shoot tip culture of banana cv. Agishwar by observing the effect of different culture conditions during induction. A micro- propagation protocol for banana ( Musa sp.) cv. Agnishwar was established by using shoot tip culture. Shoot tips obtained by removing leaf sheaths from sucker were cultured aseptically in MS (Murashige and Skoog) medium supplemented with different concentrations of cytokines viz. 6-benzylaminopurine (BAP), kinetin (kin), N 6 - (2-isopentyl) adenine (2iP) for multiplication of shoot and auxins viz. Indole-3- butyric acid (IBA), α α α α-naphthalene acetic acid (NAA) for induction of root. Maximum multiplication (95%) was obtained in MS medium containing 4.0 mg/1 BAP. The highest average number of shoots for each explant (5.9) was found in MS medium fortified with 4.0 mg/l BAP while maximum elongation of shoot (4.9cm) was observed in MS medium having 5.0 mg/l BAP. IBA at a concentration of 1.0 mg/l was found most suitable for rooting of shoot. The rooted shoots were acclimatized and successfully transferred to plastic pots. After hardening, they were transferred to the main field and the survival rate was around 90%.This protocol might be used for the massive in vitro production of the plantlets of banana cv. Agnishwar.
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Claims 6
Claim · model-written summary
Shoot multiplication of 95% was obtained in MS medium containing 4.0 mg/L BAP.
- Taxon
- bananat0
- Material
- cv. Agnishwart0
- Treatment
- 4.0 mg/1 BAPe0
- Outcome
- multiplicatione0
- Direction
- Maximume0
- Value
- 95%e0
Not reportedComparator
Verbatim
t0“Micro propagation of banana ( Musa sp.) cv. Agnishwar”
e0“Maximum multiplication (95%) was obtained in MS medium containing 4.0 mg/1 BAP”
Claim · model-written summary
4.0 mg/L BAP gave the highest average number of shoots per explant (5.9).
- Taxon
- bananat0
- Material
- cv. Agnishwart0
- Treatment
- 4.0 mg/l BAPe1
- Outcome
- average number of shoots for each explante1
- Direction
- higheste1
- Value
- 5.9e1
Not reportedComparator
Verbatim
t0“Micro propagation of banana ( Musa sp.) cv. Agnishwar”
e1“The highest average number of shoots for each explant (5.9) was found in MS medium fortified with 4.0 mg/l BAP”
Claim · model-written summary
5.0 mg/L BAP gave the maximum shoot elongation (4.9 cm).
- Taxon
- bananat0
- Material
- cv. Agnishwart0
- Treatment
- 5.0 mg/l BAPe2
- Outcome
- elongation of shoote2
- Direction
- maximume2
- Value
- 4.9cme2
Not reportedComparator
Verbatim
t0“Micro propagation of banana ( Musa sp.) cv. Agnishwar”
e2“while maximum elongation of shoot (4.9cm) was observed in MS medium having 5.0 mg/l BAP”
Claim · model-written summary
IBA at 1.0 mg/L was the most suitable auxin for rooting of shoots.
- Taxon
- bananat0
- Material
- cv. Agnishwart0
- Treatment
- IBA at a concentration of 1.0 mg/le3
- Outcome
- rooting of shoote3
- Direction
- most suitablee3
Not reportedComparator
Verbatim
t0“Micro propagation of banana ( Musa sp.) cv. Agnishwar”
e3“IBA at a concentration of 1.0 mg/l was found most suitable for rooting of shoot”
Claim · model-written summary
Acclimatized banana plantlets transferred to the main field had a survival rate of around 90%.
Verbatim
t0“Micro propagation of banana ( Musa sp.) cv. Agnishwar”
e4“After hardening, they were transferred to the main field and the survival rate was around 90%”
Claim · model-written summary
The authors suggest the protocol might be used for massive in vitro production of banana plantlets.
Verbatim
t0“Micro propagation of banana ( Musa sp.) cv. Agnishwar”
e5“This protocol might be used for the massive in vitro production of the plantlets of banana cv. Agnishwar”
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