Abstract
Ascorbate is one of the most abundant soluble antioxidants in the plant. Multiple functions of ascorbate in photo protection have been proposed, including scavenging of reactive oxygen species generated by oxygen photoreduction and photorespiration. There is still unclear information relation to LED light with Ascorbate biosynthesis and metabolism, yellowing, chlorophyll content, and ethylene production in broccoli florets. The effect of light-emitting diodes (LED) light on ascorbate (AsA) biosynthesis and metabolism in broccoli (Brassica oleracea L. var. Italica) cultivar “Ryokurei” were studied using red (660 nm), blue (470 nm) and white LED lights as the light source and also no light treatment as the control. Gene expression involved in the biosynthesis and metabolism of AsA, AsA content, color, chlorophyll content and ethylene production rate on the postharvest broccoli were observed in 4 days. The result showed that after two days, red light treatment significantly (p < 0,05) delayed the decrease of ascorbate content. The result was supported by observations using Real-Time Quantitative RT-PCR showed that red light treatment can suppress mRNA level of BO-APX1, BO-APX2, and BO-sAPX on the third day. Observation of BO-GLDH mRNA level was increased in the third-day exposure of red LED light. Therefore red LED light showed up-regulated AsA biosynthesis transcriptional level. Enzymes which possibility responsible for AsA metabolism and biosynthesis in a row were Ascorbate Peroxide (APX) and L-Galactono-1,4-Lactone Dehydrogenase (GLDH). The regulation of this gene expression might contribute to the suppression of AsA reduction by red LED light treatment in broccoli. Red LED also showed suppression of yellowing and decline the chlorophyll content in postharvest broccoli florets. Keywords: ascorbate, LED; broccoli; gene expression; real-time quantitative RT-PCR.
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Claims 5
Claim · model-written summary
Red light treatment significantly delayed the decrease of ascorbate content in broccoli compared with no light.
- Taxon
- broccoli
Brassica oleracea var. italicae0 - Treatment
- red light treatmente1
- Outcome
- ascorbate contente1
- Direction
- delayed the decreasee1
- Comparator
- no light treatmente2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in broccoli (Brassica oleracea L. var. Italica)”
e1“red light treatment significantly (p < 0,05) delayed the decrease of ascorbate content”
e2“also no light treatment as the control”
Claim · model-written summary
Red light treatment can suppress mRNA levels of BO-APX1, BO-APX2 and BO-sAPX on the third day.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- red light treatmente3
- Gene
- BO-APX1, BO-APX2, and BO-sAPXe3
- Outcome
- mRNA levele3
- Direction
- suppresse3
- Comparator
- no light treatmente2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in broccoli (Brassica oleracea L. var. Italica)”
e2“also no light treatment as the control”
e3“red light treatment can suppress mRNA level of BO-APX1, BO-APX2, and BO-sAPX on the third day”
Claim · model-written summary
BO-GLDH mRNA level increased on the third day of red LED light exposure in broccoli.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- red LED lighte4
- Gene
- BO-GLDHe4
- Outcome
- mRNA levele4
- Direction
- increasede4
- Comparator
- no light treatmente2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in broccoli (Brassica oleracea L. var. Italica)”
e2“also no light treatment as the control”
e4“Observation of BO-GLDH mRNA level was increased in the third-day exposure of red LED light”
Claim · model-written summary
Red LED light up-regulated the transcriptional level of ascorbate biosynthesis in broccoli.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- red LED lighte5
- Outcome
- AsA biosynthesis transcriptional levele5
- Direction
- up-regulatede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in broccoli (Brassica oleracea L. var. Italica)”
e5“Therefore red LED light showed up-regulated AsA biosynthesis transcriptional level”
Claim · model-written summary
Red LED light suppressed yellowing and chlorophyll content decline in postharvest broccoli florets.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Organ / stage
- floretse6
- Treatment
- Red LEDe6
- Outcome
- yellowing and decline the chlorophyll contente6
- Direction
- suppressione6
- Comparator
- no light treatmente2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in broccoli (Brassica oleracea L. var. Italica)”
e2“also no light treatment as the control”
e6“Red LED also showed suppression of yellowing and decline the chlorophyll content in postharvest broccoli florets”
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