Abstract
BACKGROUND AND OBJECTIVE: Dragon fruit (Hylocereus spp.), apple (Malus sylvestris Mill.) and tomato (Solanum lycopersicum L.) are high potential sources of antioxidant compounds such as phenolics. The compounds have the capability of protecting cells and tissues against free radicals. Secondary metabolite produced by callus cell culture from plant organs also acts as a source of antioxidants. This study aimed to determine the optimal ratio of sucrose and 2,4-D in Murashige and Skoog (MS) medium for callus induction from different plant organ explants. With all of characteristic, callus can be used further for the development of natural cell regeneration agent. METHODOLOGY: This study was conducted using analytical technique. Suitable explants were obtained. They were developed in various concentrations of combination between MS medium and 2,4-D. Callus growth, including their weight and surface was then measured and analyzed by using one-way analysis of variance (ANOVA). RESULTS: Callus was able to grow from its explants in 5-7 days after induction process. They were clear in color and had friable texture. The highest value of fresh weight of dragon fruit callus was obtained through MS supplemented with 1 μL L-1 2,4-D and 30 g sucrose. However, apple and tomato callus induction and growth maintenance reached optimal medium on MS supplemented with 30 g sucrose and 2 μL L-1 2,4-D. CONCLUSION: Callus of apple, dragon fruit and tomato was maintained upon MS supplemented with 30-40 g sucrose and 1-2 μL L-1 2,4-D for optimum induction and growth. The optimization of growth medium will give advantages for further development of natural cell regeneration agent.
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Claims 8
Claim · model-written summary
Callus grew from explants in 5-7 days.
- Taxon
- Dragon fruit
Selenicereus undatust0 - Outcome
- Callus was able to growe0
- Value
- 5-7 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus was able to grow from its explants in 5-7 days after induction process”
t0“Dragon fruit”
Claim · model-written summary
The callus was clear and friable.
- Taxon
- Dragon fruitSelenicereus undatust0
- Outcome
- clear in color and had friable texturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus was able to grow from its explants in 5-7 days after induction process. They were clear in color and had friable texture.”
t0“Dragon fruit”
Claim · model-written summary
Dragon fruit callus had its highest fresh weight on MS with 1 μL L-1 2,4-D and 30 g sucrose.
- Taxon
- dragon fruitSelenicereus undatuse0
- Treatment
- MS supplemented with 1 μL L-1 2,4-D and 30 g sucrosee0
- Outcome
- fresh weight of dragon fruit calluse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest value of fresh weight of dragon fruit callus was obtained through MS supplemented with 1 μL L-1 2,4-D and 30 g sucrose.”
Claim · model-written summary
Apple callus induction and growth maintenance were optimal on MS with 30 g sucrose and 2 μL L-1 2,4-D.
- Taxon
- apple
Malus domesticae0 - Treatment
- MS supplemented with 30 g sucrose and 2 μL L-1 2,4-De0
- Outcome
- callus induction and growth maintenancee0
- Direction
- optimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, apple and tomato callus induction and growth maintenance reached optimal medium on MS supplemented with 30 g sucrose and 2 μL L-1 2,4-D.”
Claim · model-written summary
Tomato callus induction and growth maintenance were optimal on MS with 30 g sucrose and 2 μL L-1 2,4-D.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- MS supplemented with 30 g sucrose and 2 μL L-1 2,4-De0
- Outcome
- callus induction and growth maintenancee0
- Direction
- optimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, apple and tomato callus induction and growth maintenance reached optimal medium on MS supplemented with 30 g sucrose and 2 μL L-1 2,4-D.”
Claim · model-written summary
The authors report that apple callus was maintained on MS with 30-40 g sucrose and 1-2 μL L-1 2,4-D.
- Taxon
- appleMalus domesticae0
- Treatment
- MS supplemented with 30-40 g sucrose and 1-2 μL L-1 2,4-De0
- Outcome
- maintainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus of apple, dragon fruit and tomato was maintained upon MS supplemented with 30-40 g sucrose and 1-2 μL L-1 2,4-D for optimum induction and growth.”
Claim · model-written summary
The authors report that dragon fruit callus was maintained on MS with 30-40 g sucrose and 1-2 μL L-1 2,4-D.
- Taxon
- dragon fruitSelenicereus undatuse0
- Treatment
- MS supplemented with 30-40 g sucrose and 1-2 μL L-1 2,4-De0
- Outcome
- maintainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus of apple, dragon fruit and tomato was maintained upon MS supplemented with 30-40 g sucrose and 1-2 μL L-1 2,4-D for optimum induction and growth.”
Claim · model-written summary
The authors report that tomato callus was maintained on MS with 30-40 g sucrose and 1-2 μL L-1 2,4-D.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- MS supplemented with 30-40 g sucrose and 1-2 μL L-1 2,4-De0
- Outcome
- maintainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus of apple, dragon fruit and tomato was maintained upon MS supplemented with 30-40 g sucrose and 1-2 μL L-1 2,4-D for optimum induction and growth.”
The same questions in other papers
2,4-D · growth · Malus domestica (apple)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
2,4-D · growth · Solanum lycopersicum (tomato)Positive and negative results
6 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
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11 papers in the same direction as this paper · 4 in the opposite direction
- negative
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See all 16 papers →sucrose · growth · Solanum lycopersicum (tomato)Positive and negative results
14 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
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See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.