Paper · 2016 · African Journal of Plant Science

Factors affecting in vitro degree of browning and culture establishment of pomegranate

Singh Pushpraj; R M Patel

7
claims
14
verbatim quotes
26
citations
2
research areas
✓
Open access · cc-by

Abstract

The present study was conducted to identify the most suitable types of nodal explants and browning control treatment for in vitro regeneration of pomegranate. Murashige and Skoog (MS) medium containing 1.0 mg/L BAP + 0.5 mg/L NAA was used commonly for all the treatments tested. Result revealed that the intensity of browning was increased with increased position and the length of explants. Minimum browning intensity was observed in 1st nodal explants having 1.5 cm length. However, explants of 3rd node with 2.5 cm length registered higher establishment (68.5%) and growth of explants. Furthermore, the most effective browning control was observed in subculturing of nodal explants twice, at the first day and third day of inoculation, which also found better in establishment of explants followed by activated charcoal 200 mg/L into the medium. Maximum length of shoots (3.9 cm) was recorded in 1st position of node with 2.5 cm length of explants. Key words: Nodal segments, position, antioxidants, browning, establishment.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

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Claims 7

Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Browning intensity increased with increasing explant position and length.

Taxon
pomegranatePunica granatume0
Treatment
position and the length of explantse1
Outcome
intensity of browninge1
Direction
increasede1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study was conducted to identify the most suitable types of nodal explants and browning control treatment for in vitro regeneration of pomegranate”

✓ located verbatim in the source · abstract, characters 0–157 · show in text ↑
e1

“Result revealed that the intensity of browning was increased with increased position and the length of explants”

✓ located verbatim in the source · abstract, characters 279–390 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Minimum browning intensity was observed in 1st-position nodal explants of 1.5 cm length.

Taxon
pomegranatePunica granatume0
Organ / stage
1st nodal explantse2
Treatment
1.5 cm lengthe2
Outcome
browning intensitye2
Direction
Minimume2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study was conducted to identify the most suitable types of nodal explants and browning control treatment for in vitro regeneration of pomegranate”

✓ located verbatim in the source · abstract, characters 0–157 · show in text ↑
e2

“Minimum browning intensity was observed in 1st nodal explants having 1.5 cm length”

✓ located verbatim in the source · abstract, characters 392–474 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Explants of the 3rd node with 2.5 cm length had higher establishment (68.5%).

Taxon
pomegranatePunica granatume0
Organ / stage
3rd nodee3
Treatment
2.5 cm lengthe3
Outcome
establishmente3
Direction
highere3
Value
68.5%e3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study was conducted to identify the most suitable types of nodal explants and browning control treatment for in vitro regeneration of pomegranate”

✓ located verbatim in the source · abstract, characters 0–157 · show in text ↑
e3

“explants of 3rd node with 2.5 cm length registered higher establishment (68.5%) and growth of explants”

✓ located verbatim in the source · abstract, characters 485–587 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Explants of the 3rd node with 2.5 cm length had higher growth.

Taxon
pomegranatePunica granatume0
Organ / stage
3rd nodee3
Treatment
2.5 cm lengthe3
Outcome
growth of explantse3
Direction
highere3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study was conducted to identify the most suitable types of nodal explants and browning control treatment for in vitro regeneration of pomegranate”

✓ located verbatim in the source · abstract, characters 0–157 · show in text ↑
e3

“explants of 3rd node with 2.5 cm length registered higher establishment (68.5%) and growth of explants”

✓ located verbatim in the source · abstract, characters 485–587 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Subculturing nodal explants twice was the most effective browning control.

Taxon
pomegranatePunica granatume0
Treatment
subculturing of nodal explants twicee4
Outcome
browning controle4
Direction
most effectivee4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study was conducted to identify the most suitable types of nodal explants and browning control treatment for in vitro regeneration of pomegranate”

✓ located verbatim in the source · abstract, characters 0–157 · show in text ↑
e4

“the most effective browning control was observed in subculturing of nodal explants twice, at the first day and third day of inoculation, which also found better in establishment of explants followed by activated charcoal 200 mg/L into the medium”

✓ located verbatim in the source · abstract, characters 602–847 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Subculturing nodal explants twice gave better establishment of explants.

Taxon
pomegranatePunica granatume0
Treatment
subculturing of nodal explants twicee4
Outcome
establishment of explantse4
Direction
bettere4
Qualifier
followed by activated charcoal 200 mg/Le4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study was conducted to identify the most suitable types of nodal explants and browning control treatment for in vitro regeneration of pomegranate”

✓ located verbatim in the source · abstract, characters 0–157 · show in text ↑
e4

“the most effective browning control was observed in subculturing of nodal explants twice, at the first day and third day of inoculation, which also found better in establishment of explants followed by activated charcoal 200 mg/L into the medium”

✓ located verbatim in the source · abstract, characters 602–847 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Maximum shoot length (3.9 cm) was recorded for 1st-position nodes of 2.5 cm explants.

Taxon
pomegranatePunica granatume0
Organ / stage
1st position of nodee5
Treatment
2.5 cm length of explantse5
Outcome
length of shootse5
Direction
Maximume5
Value
3.9 cme5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study was conducted to identify the most suitable types of nodal explants and browning control treatment for in vitro regeneration of pomegranate”

✓ located verbatim in the source · abstract, characters 0–157 · show in text ↑
e5

“Maximum length of shoots (3.9 cm) was recorded in 1st position of node with 2.5 cm length of explants”

✓ located verbatim in the source · abstract, characters 849–950 · show in text ↑

in vitro · growth · Punica granatum (pomegranate)Positive and negative results

25 papers: 24 positive, 1 negative or no effect

23 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. positive
  3. no effect

21 papers more in this group

See all 25 papers →

in vitro · disease · Punica granatum (pomegranate)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.