Abstract
In the present study experiments were carried out for effective control of microbial contaminants and to control the excess polyphenol exudation from the cut ends of the explants of pomegranate Cv Bhagwa under tissue culture conditions. Explants from juvenile (5-6 months old polyhouse grown plants) and mature (6-7 years old trees) plants of Cv Bhagwa were used for the study. Overall explants from juvenile plants showed less contamination compared to the explants obtained from field. Explants from juvenile plants treated with bavistin at 1000 mg/l for 15 minutes and mercuric chloride at 1000 mg/l for 10 minutes showed less fungal and bacterial contamination in cultures bottles than other treatments. Similarly explants from mature plants treated with bavistin at 1500 mg/l for 25 minutes and mercuric chloride at 1000 mg/l for 10 minutes showed less fungal and bacterial contamination in cultures bottles than other treatments. Combined Inclusion of PVP, ascorbic acid or activated charcoal in the media controlled phenol exudation but not completely. Explants were also kept in dark for 1 week to control phenolics. The sub culturing of explants thrice, at an interval of 24 h, controlled browning effectively in most of the explants.
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Claims 5
Claim · model-written summary
Explants from juvenile plants showed less contamination than explants obtained from the field.
- Taxon
- pomegranate
Punica granatumt0 - Material
- Cv. Bhagwat0
- Treatment
- juvenile plantse1
- Outcome
- contaminatione1
- Direction
- lesse1
- Comparator
- explants obtained from fielde1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of Pomegranate Cv. Bhagwa”
e1“Overall explants from juvenile plants showed less contamination compared to the explants obtained from field”
Claim · model-written summary
Bavistin at 1000 mg/l for 15 minutes plus mercuric chloride at 1000 mg/l for 10 minutes gave less fungal and bacterial contamination in juvenile-plant explants than other treatments.
- Taxon
- pomegranatePunica granatumt0
- Organ / stage
- explants from juvenile plantse2
- Treatment
- bavistin at 1000 mg/l for 15 minutes and mercuric chloride at 1000 mg/l for 10 minutese2
- Outcome
- fungal and bacterial contaminatione2
- Direction
- lesse2
- Comparator
- other treatmentse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of Pomegranate Cv. Bhagwa”
e2“Explants from juvenile plants treated with bavistin at 1000 mg/l for 15 minutes and mercuric chloride at 1000 mg/l for 10 minutes showed less fungal and bacterial contamination in cultures bottles than other treatments”
Claim · model-written summary
Bavistin at 1500 mg/l for 25 minutes plus mercuric chloride at 1000 mg/l for 10 minutes gave less fungal and bacterial contamination in mature-plant explants than other treatments.
- Taxon
- pomegranatePunica granatumt0
- Organ / stage
- explants from mature plantse3
- Treatment
- bavistin at 1500 mg/l for 25 minutes and mercuric chloride at 1000 mg/l for 10 minutese3
- Outcome
- fungal and bacterial contaminatione3
- Direction
- lesse3
- Comparator
- other treatmentse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of Pomegranate Cv. Bhagwa”
e3“Similarly explants from mature plants treated with bavistin at 1500 mg/l for 25 minutes and mercuric chloride at 1000 mg/l for 10 minutes showed less fungal and bacterial contamination in cultures bottles than other treatments”
Claim · model-written summary
Combined PVP, ascorbic acid or activated charcoal in the media controlled phenol exudation, but not completely.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- PVP, ascorbic acid or activated charcoale4
- Outcome
- phenol exudatione4
- Direction
- controllede4
- Qualifier
- but not completelye4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of Pomegranate Cv. Bhagwa”
e4“Combined Inclusion of PVP, ascorbic acid or activated charcoal in the media controlled phenol exudation but not completely”
Claim · model-written summary
Subculturing explants three times at 24 h intervals controlled browning effectively in most explants.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- sub culturing of explants thrice, at an interval of 24 he5
- Outcome
- browninge5
- Direction
- controllede5
- Qualifier
- effectively in most of the explantse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of Pomegranate Cv. Bhagwa”
e5“The sub culturing of explants thrice, at an interval of 24 h, controlled browning effectively in most of the explants”
The same questions in other papers
ascorbic acid · quality: phytochemicals · Punica granatum (pomegranate)Results agree
1 paper in the same direction as this paper
- positive
in vitro · disease · Punica granatum (pomegranate)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.