Abstract
The browning of cultures is a major obstacle in establishment of explants in perennial fruit crops, which subsequently makes the in vitro techniques more cemplicated. Pomegranate Punica granatum L. has the same problem in establishment of in vitro cultures due to high phenolic contents. Several treatments were tried including use of adsorbents and antioxidants with different explants viz., leaf segment, cotyledon, nodal segment and shoot tip. The activated charcoal (adsorbent) and ascorbic acid (antioxidant) could not alter the extent of browning at various concentrations. The subculturing of explants thrice, at an interval of 24 hrs. controlled browning in all explants used, except cotyledon. The cotyledon explant was deprived of any browning secretion.
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Claims 4
Claim · model-written summary
Activated charcoal did not alter browning at the tested concentrations.
- Taxon
- Pomegranate Punica granatum L.
Punica granatume1 - Treatment
- activated charcoale0
- Outcome
- extent of browninge0
- Direction
- could not altere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activated charcoal (adsorbent) and ascorbic acid (antioxidant) could not alter the extent of browning at various concentrations”
e1“Pomegranate Punica granatum L.”
Claim · model-written summary
Ascorbic acid did not alter browning at the tested concentrations.
- Taxon
- Pomegranate Punica granatum L.Punica granatume1
- Treatment
- ascorbic acide0
- Outcome
- extent of browninge0
- Direction
- could not altere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activated charcoal (adsorbent) and ascorbic acid (antioxidant) could not alter the extent of browning at various concentrations”
e1“Pomegranate Punica granatum L.”
Claim · model-written summary
Three subcultures at 24-hour intervals controlled browning in explants other than cotyledons.
- Taxon
- Pomegranate Punica granatum L.Punica granatume1
- Treatment
- subculturing of explants thrice, at an interval of 24 hrs.e0
- Outcome
- browninge0
- Direction
- controllede0
- Qualifier
- except cotyledone0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The subculturing of explants thrice, at an interval of 24 hrs. controlled browning in all explants used, except cotyledon”
e1“Pomegranate Punica granatum L.”
Claim · model-written summary
Cotyledon explants showed no browning secretion.
- Taxon
- Pomegranate Punica granatum L.Punica granatume1
- Organ / stage
- cotyledon explante0
- Outcome
- deprived of any browning secretione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cotyledon explant was deprived of any browning secretion”
e1“Pomegranate Punica granatum L.”
The same questions in other papers
charcoal · disease · Punica granatum (pomegranate)Positive and negative results
1 in the opposite direction
- positive
in vitro · disease · Punica granatum (pomegranate)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.