Paper · 2019 · International Journal of Fruit Science

Micropropagation of Arid Zone Fruit Tree, Pomegranate, cvs. ‘Malase Yazdi’ and ‘Shirine Shahvar’

Sara Mulaei; Azam Jafari; Mostafa Shirmardi; et al.

7
claims
13
verbatim quotes
10
citations
3
research areas
✓
Open access

Abstract

An optimum culture medium for micropropagation of pomegranate, cvs. ‘Malase Yazdi’ and ‘Shirine Shahvar’ is defined and protocols have been developed for in vitro shoot proliferation and rooting of the cultivars. Axillary buds of young shoots were used as explants. The shoots were multiplied in vitro on Murashige and Skoog (MS) supplemented with 0, 1.5, 2, and 2.5 mg.l−1 of N6-benzyladenine (BA). The micropropagated/cloned shoots were rooted on MS and half strength (1/2) of MS containing indole-3-butyric acid (IBA) at three concentrations (0, 0.5, and 1 mg.l−1) and 0.5 mg.l−1 naphthalene acetic acid (NAA). The cultivar ‘Malase Yazdi’ multiplied better than ‘Shirine Shahvar’. In both cultivars, the maximum shoot number with maximum shoot height was produced on medium with 2 mg.l−1 BA. Control medium free from plant growth regulator also supported shoot multiplication. The cultured shoots exhibited the greatest number of leaves on a medium containing 2.5 mg.l−1 BA. The cloned shoots of ‘Malase Yazdi’ rooted the best on half-strength MS medium with 0.5 mg.l−1 IBA while shoots of ‘Shirine Shahvar’, rooted on 1 mg.l−1 IBA and 0.5 mg.l−1 NAA in half MS medium. This method can be used to quickly propagate and conservation of these two pomegranate cultivars for future prospects.

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Claims 7

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Malase Yazdi multiplied better in vitro than Shirine Shahvar.

Taxon
PomegranatePunica granatumt0
Material
‘Malase Yazdi’e0
Outcome
multipliede0
Direction
bettere0
Comparator
‘Shirine Shahvar’e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Pomegranate”

✓ located verbatim in the source · title, characters 42–53 · show in text ↑
e0

“The cultivar ‘Malase Yazdi’ multiplied better than ‘Shirine Shahvar’.”

✓ located verbatim in the source · abstract, characters 614–683 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Both cultivars produced maximum shoot number and height with 2 mg.l−1 BA.

Taxon
PomegranatePunica granatumt0
Treatment
2 mg.l−1 BAe0
Outcome
maximum shoot number with maximum shoot heighte0
Direction
maximume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Pomegranate”

✓ located verbatim in the source · title, characters 42–53 · show in text ↑
e0

“In both cultivars, the maximum shoot number with maximum shoot height was produced on medium with 2 mg.l−1 BA.”

✓ located verbatim in the source · abstract, characters 684–794 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Growth regulator free control medium supported shoot multiplication.

Taxon
PomegranatePunica granatumt0
Treatment
Control medium free from plant growth regulatore0
Outcome
shoot multiplicatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Pomegranate”

✓ located verbatim in the source · title, characters 42–53 · show in text ↑
e0

“Control medium free from plant growth regulator also supported shoot multiplication.”

✓ located verbatim in the source · abstract, characters 795–879 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cultured shoots had the most leaves with 2.5 mg.l−1 BA.

Taxon
PomegranatePunica granatumt0
Treatment
2.5 mg.l−1 BAe0
Outcome
number of leavese0
Direction
greateste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Pomegranate”

✓ located verbatim in the source · title, characters 42–53 · show in text ↑
e0

“The cultured shoots exhibited the greatest number of leaves on a medium containing 2.5 mg.l−1 BA.”

✓ located verbatim in the source · abstract, characters 880–977 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

‘Malase Yazdi’ rooted under half-strength MS medium with 0.5 mg.l−1 IBA.

Taxon
PomegranatePunica granatumt0
Material
‘Malase Yazdi’e0
Treatment
half-strength MS medium with 0.5 mg.l−1 IBAe0
Outcome
rootede0
Direction
beste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Pomegranate”

✓ located verbatim in the source · title, characters 42–53 · show in text ↑
e0

“The cloned shoots of ‘Malase Yazdi’ rooted the best on half-strength MS medium with 0.5 mg.l−1 IBA”

✓ located verbatim in the source · abstract, characters 978–1076 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

‘Shirine Shahvar’ rooted under 1 mg.l−1 IBA and 0.5 mg.l−1 NAA in half MS medium.

Taxon
PomegranatePunica granatumt0
Material
‘Shirine Shahvar’e0
Treatment
1 mg.l−1 IBA and 0.5 mg.l−1 NAA in half MS mediume0
Outcome
rootede0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Pomegranate”

✓ located verbatim in the source · title, characters 42–53 · show in text ↑
e0

“shoots of ‘Shirine Shahvar’, rooted on 1 mg.l−1 IBA and 0.5 mg.l−1 NAA in half MS medium”

✓ located verbatim in the source · abstract, characters 1083–1171 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors recommend the protocol for rapid propagation and conservation of the two pomegranate cultivars.

Taxon
pomegranatePunica granatume0
Outcome
quickly propagate and conservatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“This method can be used to quickly propagate and conservation of these two pomegranate cultivars for future prospects.”

✓ located verbatim in the source · abstract, characters 1173–1291 · show in text ↑

N-benzyladenine · growth · Punica granatum (pomegranate)Results agree

24 papers: 24 positive, 0 negative or no effect

23 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

20 papers more in this group

See all 24 papers →

1-naphthaleneacetic acid · growth · Punica granatum (pomegranate)Results agree

25 papers: 25 positive, 0 negative or no effect

24 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

21 papers more in this group

See all 25 papers →

indole-3-butyric acid · growth · Punica granatum (pomegranate)Results agree

38 papers: 38 positive, 0 negative or no effect

37 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

34 papers more in this group

See all 38 papers →

in vitro · growth · Punica granatum (pomegranate)Positive and negative results

25 papers: 24 positive, 1 negative or no effect

23 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. positive
  3. positive

21 papers more in this group

See all 25 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.