Abstract
An optimum culture medium for micropropagation of pomegranate, cvs. ‘Malase Yazdi’ and ‘Shirine Shahvar’ is defined and protocols have been developed for in vitro shoot proliferation and rooting of the cultivars. Axillary buds of young shoots were used as explants. The shoots were multiplied in vitro on Murashige and Skoog (MS) supplemented with 0, 1.5, 2, and 2.5 mg.l−1 of N6-benzyladenine (BA). The micropropagated/cloned shoots were rooted on MS and half strength (1/2) of MS containing indole-3-butyric acid (IBA) at three concentrations (0, 0.5, and 1 mg.l−1) and 0.5 mg.l−1 naphthalene acetic acid (NAA). The cultivar ‘Malase Yazdi’ multiplied better than ‘Shirine Shahvar’. In both cultivars, the maximum shoot number with maximum shoot height was produced on medium with 2 mg.l−1 BA. Control medium free from plant growth regulator also supported shoot multiplication. The cultured shoots exhibited the greatest number of leaves on a medium containing 2.5 mg.l−1 BA. The cloned shoots of ‘Malase Yazdi’ rooted the best on half-strength MS medium with 0.5 mg.l−1 IBA while shoots of ‘Shirine Shahvar’, rooted on 1 mg.l−1 IBA and 0.5 mg.l−1 NAA in half MS medium. This method can be used to quickly propagate and conservation of these two pomegranate cultivars for future prospects.
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Claims 7
Claim · model-written summary
Malase Yazdi multiplied better in vitro than Shirine Shahvar.
- Taxon
- Pomegranate
Punica granatumt0
- Material
- ‘Malase Yazdi’e0
- Outcome
- multipliede0
- Direction
- bettere0
- Comparator
- ‘Shirine Shahvar’e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate”
e0“The cultivar ‘Malase Yazdi’ multiplied better than ‘Shirine Shahvar’.”
Claim · model-written summary
Both cultivars produced maximum shoot number and height with 2 mg.l−1 BA.
- Taxon
- Pomegranate
Punica granatumt0
- Treatment
- 2 mg.l−1 BAe0
- Outcome
- maximum shoot number with maximum shoot heighte0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate”
e0“In both cultivars, the maximum shoot number with maximum shoot height was produced on medium with 2 mg.l−1 BA.”
Claim · model-written summary
Growth regulator free control medium supported shoot multiplication.
- Taxon
- Pomegranate
Punica granatumt0
- Treatment
- Control medium free from plant growth regulatore0
- Outcome
- shoot multiplicatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate”
e0“Control medium free from plant growth regulator also supported shoot multiplication.”
Claim · model-written summary
Cultured shoots had the most leaves with 2.5 mg.l−1 BA.
- Taxon
- Pomegranate
Punica granatumt0
- Treatment
- 2.5 mg.l−1 BAe0
- Outcome
- number of leavese0
- Direction
- greateste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate”
e0“The cultured shoots exhibited the greatest number of leaves on a medium containing 2.5 mg.l−1 BA.”
Claim · model-written summary
‘Malase Yazdi’ rooted under half-strength MS medium with 0.5 mg.l−1 IBA.
- Taxon
- Pomegranate
Punica granatumt0
- Material
- ‘Malase Yazdi’e0
- Treatment
- half-strength MS medium with 0.5 mg.l−1 IBAe0
- Outcome
- rootede0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate”
e0“The cloned shoots of ‘Malase Yazdi’ rooted the best on half-strength MS medium with 0.5 mg.l−1 IBA”
Claim · model-written summary
‘Shirine Shahvar’ rooted under 1 mg.l−1 IBA and 0.5 mg.l−1 NAA in half MS medium.
- Taxon
- Pomegranate
Punica granatumt0
- Material
- ‘Shirine Shahvar’e0
- Treatment
- 1 mg.l−1 IBA and 0.5 mg.l−1 NAA in half MS mediume0
- Outcome
- rootede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate”
e0“shoots of ‘Shirine Shahvar’, rooted on 1 mg.l−1 IBA and 0.5 mg.l−1 NAA in half MS medium”
Claim · model-written summary
The authors recommend the protocol for rapid propagation and conservation of the two pomegranate cultivars.
- Taxon
- pomegranate
Punica granatume0
- Outcome
- quickly propagate and conservatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This method can be used to quickly propagate and conservation of these two pomegranate cultivars for future prospects.”
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