Abstract
The conventional method of propagation of pomegranate is time-consuming and tiresome. It does not ensure disease-free and healthy plants. The Present investigation aimed to optimize the conditions of culture medium, shoot proliferation, and rooting for mass production of pomegranate trees (Punica granatum L.) cv. ʻWonderful Cultivarʼ. For sterilization of axillary bud explants and shoot apexes of wonderful pomegranate treatment involving HgCl2 (0.1 %) for 3 min gave better sterilization. An efficient in vitro propagation for wonderful pomegranate using shoot tip and axillary bud explants is described. The best media for the establishment was observed on DKW medium for the first three weeks. After that, explants were subcultured on a WPM medium for proliferation and elongation. ½MS and full MS were used for rooting. The maximum number of shoots (3.9) per explant and shoot length (1.5 to 2.95 cm) were obtained on WPM medium supplemented with 0.8 mg/l B A P + 0.01 mg/L IBA in the medium. The highest rooting number was recorded on ½MS medium containing IBA 1mg/l—(1.4) roots per explant. A significantly higher number of shoots (2.5) and maximum length (2.95cm) of the shoot were recorded in 3 percent sucrose, (3000 lux) light intensity, and pH 5.8. Rooted plantlets were adopted and transferred to the soil successfully.
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Claims 8
Claim · model-written summary
better sterilization was optimized under HgCl2 (0.1 %) for 3 min.
- Taxon
- pomegranate
Punica granatumt0 - Treatment
- HgCl2 (0.1 %) for 3 mine0
- Outcome
- better sterilizatione0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HgCl2 (0.1 %) for 3 min gave better sterilization”
t0“Pomegranate (Punica Granatum L.)”
Claim · model-written summary
establishment was optimized under DKW medium.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- DKW mediume0
- Outcome
- establishmente0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best media for the establishment was observed on DKW medium for the first three weeks”
t0“Pomegranate (Punica Granatum L.)”
Claim · model-written summary
number of shoots was optimized under WPM medium supplemented with 0.8 mg/l B A P + 0.01 mg/L IBA.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- WPM medium supplemented with 0.8 mg/l B A P + 0.01 mg/L IBAe0
- Outcome
- number of shootse0
- Direction
- maximume0
- Value
- 3.9e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum number of shoots (3.9) per explant and shoot length (1.5 to 2.95 cm) were obtained on WPM medium supplemented with 0.8 mg/l B A P + 0.01 mg/L IBA in the medium”
t0“Pomegranate (Punica Granatum L.)”
Claim · model-written summary
shoot length was optimized under WPM medium supplemented with 0.8 mg/l B A P + 0.01 mg/L IBA.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- WPM medium supplemented with 0.8 mg/l B A P + 0.01 mg/L IBAe0
- Outcome
- shoot lengthe0
- Direction
- maximume0
- Value
- 1.5 to 2.95 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum number of shoots (3.9) per explant and shoot length (1.5 to 2.95 cm) were obtained on WPM medium supplemented with 0.8 mg/l B A P + 0.01 mg/L IBA in the medium”
t0“Pomegranate (Punica Granatum L.)”
Claim · model-written summary
rooting number was optimized under ½MS medium containing IBA 1mg/l.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- ½MS medium containing IBA 1mg/le0
- Outcome
- rooting numbere0
- Direction
- higheste0
- Value
- 1.4e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest rooting number was recorded on ½MS medium containing IBA 1mg/l—(1.4) roots per explant”
t0“Pomegranate (Punica Granatum L.)”
Claim · model-written summary
number of shoots was optimized under 3 percent sucrose, (3000 lux) light intensity, and pH 5.8.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- 3 percent sucrose, (3000 lux) light intensity, and pH 5.8e0
- Outcome
- number of shootse0
- Direction
- highere0
- Value
- 2.5e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significantly higher number of shoots (2.5) and maximum length (2.95cm) of the shoot were recorded in 3 percent sucrose, (3000 lux) light intensity, and pH 5.8”
t0“Pomegranate (Punica Granatum L.)”
Claim · model-written summary
length was optimized under 3 percent sucrose, (3000 lux) light intensity, and pH 5.8.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- 3 percent sucrose, (3000 lux) light intensity, and pH 5.8e0
- Outcome
- lengthe0
- Direction
- maximume0
- Value
- 2.95cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significantly higher number of shoots (2.5) and maximum length (2.95cm) of the shoot were recorded in 3 percent sucrose, (3000 lux) light intensity, and pH 5.8”
t0“Pomegranate (Punica Granatum L.)”
Claim · model-written summary
Rooted plantlets were successfully transferred to soil.
- Taxon
- pomegranatePunica granatumt0
- Outcome
- transferred to the soil successfullye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rooted plantlets were adopted and transferred to the soil successfully.”
t0“Pomegranate (Punica Granatum L.)”
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See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.