Abstract
The barley variety,LSY(CK)and its transgenic line overexpressing Trxs(LSY-11-1-1,T)were employed to measure the Fv/Fm value,contents of H2O2 and malonaldehyde(MDA),and the antioxidant enzyme activity under ultraviolet B(UV-B) radiation stress with hydroponic method.The results showed that the Fv/Fm value in leaves of barley seedling treated with UV-B radiation was significantly lower than that in the samples untreated(P0.01),and the content of H2O2 and MDA was significantly increased by UV-B radiation(P0.01).Activities of superoxide dismutase(SOD),guaiacol peroxidase(POD),glutathione peroxides(GSH-PX) and catalase(CAT) give a rise to different degrees under the UV-B stress.In comparison with UVB-CK,the Fv/Fm value of UVB-T was significantly higher,but the content of H2O2 and MDA of UVB-T was significantly lower than UVB-CK(P0.01).Activities of antioxidant enzymes given above in UVB-T leaves were generally higher than UVB-CK.Moreover,the activity change tendency of CAT in UVB-T and UVB-CK was different,as there are two activity peaks in the former,but only one in the latter.
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Claims 10
Claim · model-written summary
UV-B reduced barley leaf Fv/Fm versus untreated samples.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- UV-B radiatione0
- Outcome
- Fv/Fm valuee0
- Direction
- lowere0
- Value
- P0.01e0
- Comparator
- samples untreatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“the Fv/Fm value in leaves of barley seedling treated with UV-B radiation was significantly lower than that in the samples untreated(P0.01)”
Claim · model-written summary
UV-B increased barley H2O2 content.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- UV-B radiatione0
- Outcome
- H2O2e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the content of H2O2 and MDA was significantly increased by UV-B radiation(P0.01)”
t0“barley”
Claim · model-written summary
UV-B increased barley MDA content.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- UV-B radiatione0
- Outcome
- MDAe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the content of H2O2 and MDA was significantly increased by UV-B radiation(P0.01)”
t0“barley”
Claim · model-written summary
UV-B raised activities of SOD, POD and GSH-PX.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- UV-B stresse0
- Outcome
- superoxide dismutase(SOD),guaiacol peroxidase(POD),glutathione peroxides(GSH-PX)e0
- Direction
- risee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activities of superoxide dismutase(SOD),guaiacol peroxidase(POD),glutathione peroxides(GSH-PX) and catalase(CAT) give a rise to different degrees under the UV-B stress.”
t0“barley”
Claim · model-written summary
UV-B raised CAT activity.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- UV-B stresse0
- Outcome
- catalase(CAT)e0
- Direction
- risee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activities of superoxide dismutase(SOD),guaiacol peroxidase(POD),glutathione peroxides(GSH-PX) and catalase(CAT) give a rise to different degrees under the UV-B stress.”
t0“barley”
Claim · model-written summary
Under UV-B, the Trxs line had higher Fv/Fm than control.
- Taxon
- barleyHordeum vulgaret0
- Material
- UVB-Te0
- Treatment
- UVB-Te0
- Outcome
- Fv/Fm valuee0
- Direction
- highere0
- Comparator
- UVB-CKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In comparison with UVB-CK,the Fv/Fm value of UVB-T was significantly higher”
t0“barley”
Claim · model-written summary
Under UV-B, the Trxs line had lower H2O2 content than control.
- Taxon
- barleyHordeum vulgaret0
- Material
- UVB-Te0
- Treatment
- UVB-Te0
- Outcome
- H2O2e0
- Direction
- lowere0
- Comparator
- UVB-CKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the content of H2O2 and MDA of UVB-T was significantly lower than UVB-CK(P0.01)”
t0“barley”
Claim · model-written summary
Under UV-B, the Trxs line had lower MDA content than control.
- Taxon
- barleyHordeum vulgaret0
- Material
- UVB-Te0
- Treatment
- UVB-Te0
- Outcome
- MDAe0
- Direction
- lowere0
- Comparator
- UVB-CKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the content of H2O2 and MDA of UVB-T was significantly lower than UVB-CK(P0.01)”
t0“barley”
Claim · model-written summary
Antioxidant enzyme activities in UVB-T leaves were generally higher than UVB-CK.
- Taxon
- barleyHordeum vulgaret0
- Material
- UVB-Te0
- Treatment
- UVB-Te0
- Outcome
- Activities of antioxidant enzymese0
- Direction
- highere0
- Comparator
- UVB-CKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activities of antioxidant enzymes given above in UVB-T leaves were generally higher than UVB-CK.”
t0“barley”
Claim · model-written summary
CAT activity had two peaks in UVB-T and one in UVB-CK.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- activity peakse0
- Value
- two activity peakse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there are two activity peaks in the former,but only one in the latter.”
t0“barley”
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