Abstract
Calmodulin (CaM) is a multifunctional Ca2+-binding protein in plant cells. It plays important roles in regulating the growth, development and disease resistance in plants. A full-length cDNA sequence coding for CaM in common bean was cloned based on expressed sequence tags from common bean. Sequence analysis showed that the isolated fragment was 713 bp, it con-tained an open reading frame (ORF) of 453 bp encoding 150 amino acids with a theoretical molecular weight of 17.16 kD, desig-nated PvCaM1 (GenBank accession number JN418801). Online ScanProsite tool analysis showed that PvCaM1 had four Ca2+-binding domains with the function of combining with free Ca2+. Homology analysis indicated that PvCaM1 gene was simi-lar to CaM genes in other plant species including Lotus japonicus (CAB63264.3), watermelon (BAI52955.1), Populus (ADC80735.1) and castor bean (XP_002533357.1).Phylogenetic analysis based on the amino acids sequence of PvCaM1 with other nine species showed that the protein encoded by this gene had the closest relationship with the CaM in Lotus japonicus and watermelon, the homology were 77% and 76%, respectively. Real time-PCR analysis indicated that the expression level of PvCaM1 in the interactions between the resistant cultivars and Fusarium wilt pathogen FOP-DM01 (Fusarium oxysporum f. sp. phaseoli) increased significantly and reached the peak at 96 h, however, the susceptible one touched the bottom under the same conditions. The expression level of PvCaM1 in 260205 leaves was higher than that in BRB-130 at all different time points. PvCaM1 expressed differently in the leaves, stems and roots, the expression level in leaves was higher than that in roots and stems. Transcriptional level of PvCaM1 was up-regulated by exogenous abscisic acid, methyl jasmonate and ethephon, but not increased significantly by salicylic acid and 3-indoleacetic acid. These results suggested that PvCaM1 is probably involved in the abscisic acid, jasmonic acid and ethylene-regulated resistant response pathways, but not closely related to the salicylic acid and 3-indoleacetic acid path-way. The study indicated that the function of PvCaM1 should be closely related to the resistant response pathways against Fusa-rium wilt pathogen FOP-DM01 with involvement of abscisic acid, jasmonic acid and ethylene in common bean.
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Claims 13
Claim · model-written summary
A full-length PvCaM1 cDNA fragment of 713 bp was cloned from common bean, containing a 453 bp open reading frame encoding 150 amino acids.
- Taxon
- Common Bean
Phaseolus vulgarist0 - Gene
- PvCaM1e0
- Outcome
- open reading frame (ORF) of 453 bp encoding 150 amino acidse0
- Value
- 713 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean (Phaseolus vulgaris L.)”
e0“Sequence analysis showed that the isolated fragment was 713 bp, it con-tained an open reading frame (ORF) of 453 bp encoding 150 amino acids with a theoretical molecular weight of 17.16 kD, desig-nated PvCaM1”
Claim · model-written summary
PvCaM1 has four Ca2+-binding domains.
- Taxon
- Common BeanPhaseolus vulgarist0
- Gene
- PvCaM1e0
- Outcome
- four Ca2+-binding domainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean (Phaseolus vulgaris L.)”
e0“PvCaM1 had four Ca2+-binding domains”
Claim · model-written summary
PvCaM1 is similar to CaM genes of Lotus japonicus, watermelon, Populus and castor bean.
- Taxon
- Common BeanPhaseolus vulgarist0
- Gene
- PvCaM1e0
- Outcome
- simi-lar to CaM genes in other plant speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean (Phaseolus vulgaris L.)”
e0“PvCaM1 gene was simi-lar to CaM genes in other plant species including Lotus japonicus (CAB63264.3), watermelon (BAI52955.1), Populus (ADC80735.1) and castor bean (XP_002533357.1)”
Claim · model-written summary
The PvCaM1 protein is most closely related to the CaM of Lotus japonicus, with 77% homology.
- Taxon
- Common BeanPhaseolus vulgarist0
- Outcome
- homologye0
- Value
- 77%e0
- Comparator
- Lotus japonicuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean (Phaseolus vulgaris L.)”
e0“the protein encoded by this gene had the closest relationship with the CaM in Lotus japonicus and watermelon, the homology were 77% and 76%, respectively”
Claim · model-written summary
The PvCaM1 protein is also closely related to the CaM of watermelon, with 76% homology.
- Taxon
- Common BeanPhaseolus vulgarist0
- Outcome
- homologye0
- Value
- 76%e0
- Comparator
- watermelone0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean (Phaseolus vulgaris L.)”
e0“the protein encoded by this gene had the closest relationship with the CaM in Lotus japonicus and watermelon, the homology were 77% and 76%, respectively”
Claim · model-written summary
PvCaM1 expression in resistant cultivars infected with Fusarium oxysporum f. sp. phaseoli FOP-DM01 increased significantly and peaked at 96 h.
- Taxon
- Common BeanPhaseolus vulgarist0
- Material
- resistant cultivarse0
- Treatment
- Fusarium wilt pathogen FOP-DM01e0
- Interactor
- Fusarium oxysporum f. sp. phaseoli
Fusarium oxysporum f. sp. phaseolie0pathogen - Gene
- PvCaM1e0
- Outcome
- expression level of PvCaM1e0
- Direction
- increased significantlye0
- Qualifier
- reached the peak at 96 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean (Phaseolus vulgaris L.)”
e0“the expression level of PvCaM1 in the interactions between the resistant cultivars and Fusarium wilt pathogen FOP-DM01 (Fusarium oxysporum f. sp. phaseoli) increased significantly and reached the peak at 96 h”
Claim · model-written summary
In the susceptible cultivar under the same pathogen conditions PvCaM1 expression touched the bottom.
- Taxon
- Common BeanPhaseolus vulgarist0
- Material
- susceptible onee0
- Treatment
- Fusarium wilt pathogen FOP-DM01e0
- Interactor
- Fusarium oxysporum f. sp. phaseoliFusarium oxysporum f. sp. phaseolie0pathogen
- Gene
- PvCaM1e0
- Outcome
- expression level of PvCaM1e0
- Direction
- touched the bottome0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean (Phaseolus vulgaris L.)”
e0“the expression level of PvCaM1 in the interactions between the resistant cultivars and Fusarium wilt pathogen FOP-DM01 (Fusarium oxysporum f. sp. phaseoli) increased significantly and reached the peak at 96 h, however, the susceptible one touched the bottom under the same conditions”
Claim · model-written summary
PvCaM1 expression in 260205 leaves was higher than in BRB-130 at all time points.
- Taxon
- Common BeanPhaseolus vulgarist0
- Material
- 260205e0
- Organ / stage
- leavese0
- Gene
- PvCaM1e0
- Outcome
- expression level of PvCaM1e0
- Direction
- highere0
- Comparator
- BRB-130e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean (Phaseolus vulgaris L.)”
e0“The expression level of PvCaM1 in 260205 leaves was higher than that in BRB-130 at all different time points”
Claim · model-written summary
PvCaM1 expression was higher in leaves than in roots and stems.
- Taxon
- Common BeanPhaseolus vulgarist0
- Organ / stage
- leavese0
- Gene
- PvCaM1e0
- Outcome
- expression levele0
- Direction
- highere0
- Comparator
- roots and stemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean (Phaseolus vulgaris L.)”
e0“PvCaM1 expressed differently in the leaves, stems and roots, the expression level in leaves was higher than that in roots and stems”
Claim · model-written summary
PvCaM1 transcript level was up-regulated by exogenous abscisic acid, methyl jasmonate and ethephon.
- Taxon
- Common BeanPhaseolus vulgarist0
- Treatment
- exogenous abscisic acid, methyl jasmonate and ethephone0
- Gene
- PvCaM1e0
- Outcome
- Transcriptional level of PvCaM1e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean (Phaseolus vulgaris L.)”
e0“Transcriptional level of PvCaM1 was up-regulated by exogenous abscisic acid, methyl jasmonate and ethephon”
Claim · model-written summary
PvCaM1 transcript level was not increased significantly by salicylic acid and 3-indoleacetic acid.
- Taxon
- Common BeanPhaseolus vulgarist0
- Treatment
- salicylic acid and 3-indoleacetic acide0
- Gene
- PvCaM1e0
- Outcome
- Transcriptional level of PvCaM1e0
- Direction
- not increased significantlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean (Phaseolus vulgaris L.)”
e0“Transcriptional level of PvCaM1 was up-regulated by exogenous abscisic acid, methyl jasmonate and ethephon, but not increased significantly by salicylic acid and 3-indoleacetic acid”
Claim · model-written summary
The authors suggest PvCaM1 is probably involved in abscisic acid, jasmonic acid and ethylene-regulated resistant response pathways.
- Taxon
- Common BeanPhaseolus vulgarist0
- Gene
- PvCaM1e0
- Compound
- abscisic acid, jasmonic acid and ethylenee0
- Outcome
- resistant response pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean (Phaseolus vulgaris L.)”
e0“PvCaM1 is probably involved in the abscisic acid, jasmonic acid and ethylene-regulated resistant response pathways”
Claim · model-written summary
The authors suggest PvCaM1 is not closely related to the salicylic acid and 3-indoleacetic acid pathway.
- Taxon
- Common BeanPhaseolus vulgarist0
- Gene
- PvCaM1e0
- Compound
- salicylic acid and 3-indoleacetic acide0
- Outcome
- resistant response pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean (Phaseolus vulgaris L.)”
e0“PvCaM1 is probably involved in the abscisic acid, jasmonic acid and ethylene-regulated resistant response pathways, but not closely related to the salicylic acid and 3-indoleacetic acid path-way”
The same questions in other papers
(-)-methyl jasmonate · molecular: expression · Phaseolus vulgaris (common bean)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →salicylic acid · molecular: expression · Phaseolus vulgaris (common bean)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.