Abstract
Abstract An increase in ultraviolet (UV) B radiation on the earth's surface is a feature of current global climate changes. It has been reported that alternative oxidase (AOX) may have a protective role against oxidative stress induced by environmental stresses, such as UV‐B. To better understand the characteristic tolerance of plants to UV‐B radiation, the effects of enhanced UV‐B radiation on the activity and expression of AOX in red kidney bean ( Phaseolus vulgaris ) leaves were investigated in the present study. The results show that the total respiration rate and AOX activity in red kidney bean leaves increased significantly during treatment with enhanced UV‐B. However, cytochrome oxidase (COX) activity did not change significantly. The H 2 O 2 content was also markedly increased and reached a maximum of 4.45 mmol·L −1 ·g −1 DW (dry weight) at 24 h of UV‐B treatment, before dropping rapidly. Both alternative pathway content and alternative pathway activity were increased in the presence of exogenous H 2 O 2 . Immunoblotting analysis with anti‐AOX monoclonal antibody revealed that expression of the AOX protein increased in red kidney bean leaves under enhanced UV‐B radiation, reaching a peak at 72 h. In addition, AOX expression in red kidney bean leaves was induced by exogenous H 2 O 2 . These data indicate that the increase in AOX activity in red kidney bean leaves under enhanced UV‐B radiation was mainly due to H 2 O 2 ‐induced AOX expression.
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Claims 7
Claim · model-written summary
UV-B increased total leaf respiration and AOX activity.
- Taxon
- red kidney beane0
- Treatment
- enhanced UV‐Be0
- Outcome
- total respiration rate and AOX activitye0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“the total respiration rate and AOX activity in red kidney bean leaves increased significantly during treatment with enhanced UV‐B.”
Claim · model-written summary
COX activity did not change significantly.
Verbatim
e0“cytochrome oxidase (COX) activity did not change significantly.”
t0“Red Kidney Bean”
Claim · model-written summary
Hydrogen peroxide increased and peaked at 24 hours.
Verbatim
e0“The H 2 O 2 content was also markedly increased and reached a maximum of 4.45 mmol·L −1 ·g −1 DW (dry weight) at 24 h of UV‐B treatment”
t0“Red Kidney Bean”
Claim · model-written summary
Exogenous peroxide increased alternative pathway content and activity.
- Taxon
- red kidney beant0
- Treatment
- exogenous H 2 O 2e0
- Outcome
- alternative pathway content and alternative pathway activitye0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“Both alternative pathway content and alternative pathway activity were increased in the presence of exogenous H 2 O 2 .”
t0“Red Kidney Bean”
Claim · model-written summary
AOX protein expression increased under enhanced UV-B and peaked at 72 hours.
- Taxon
- red kidney beane0
- Treatment
- enhanced UV‐B radiatione0
- Gene
- AOX proteine0
- Outcome
- expression of the AOX proteine0
- Direction
- increasede0
- Qualifier
- 72 he0
Not reportedComparator
Verbatim
e0“expression of the AOX protein increased in red kidney bean leaves under enhanced UV‐B radiation, reaching a peak at 72 h.”
Claim · model-written summary
Exogenous peroxide induced AOX expression.
- Taxon
- red kidney beane0
- Treatment
- exogenous H 2 O 2e0
- Gene
- AOXe0
- Outcome
- AOX expressione0
- Direction
- inducede0
Not reportedComparator
Verbatim
e0“AOX expression in red kidney bean leaves was induced by exogenous H 2 O 2 .”
Claim · model-written summary
Authors attributed the UV-B AOX activity increase mainly to peroxide-induced AOX expression.
Verbatim
e0“the increase in AOX activity in red kidney bean leaves under enhanced UV‐B radiation was mainly due to H 2 O 2 ‐induced AOX expression.”
The same questions in other papers
uv · physiology: respiration · Phaseolus vulgaris (common bean)Positive and negative results
1 in the opposite direction
- negative
uv · molecular: expression · Phaseolus vulgaris (common bean)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.