Abstract
The hypocotyl,cotyledon,petiole and leaf of five Chinese local alfalfa cultivars were used to study the effect of different basic mediums,different hormone combinations,different proline,sucrose concentrations and active carbon on embryogenic callus and somatic embryos induction and differentiation.The results indicated that MS containing 5.0 mg/L 2,4-D and 1.0 mg/L KT(MSH) was the optimum embryonic callus induction medium.Hypocotyl was the best explant for rapid callus induction.The hypocotyl callus induction rate of Longdong and Daye was close to 100% on MSH.SH medium was the most efficient for inducing somatic embryos,on which,the somatic embryos induction rate of Longdong and Daye was up to 72% and 58% respectively.1/2MS with 10 g/L sucrose was propitious for rooting and seedling of somatic embryos.Proline had no effect on induction and differentiation of somatic embryos,adding active carbon in SH medium can reduce somatic embryos induction rates,the highest somatic embryos induction rate for sucrose was 20 g/L.The establishment of high frequency regeneration system of alfalfa somatic embryos benefits the AtNHX1 gene transformation of alfalfa greatly.
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Claims 12
Claim · model-written summary
MSH was the optimum medium for embryogenic callus induction.
- Taxon
- alfalfa
Medicago sativat0 - Treatment
- MS containing 5.0 mg/L 2,4-D and 1.0 mg/L KT(MSH)e0
- Outcome
- embryonic callus inductione0
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results indicated that MS containing 5.0 mg/L 2,4-D and 1.0 mg/L KT(MSH) was the optimum embryonic callus induction medium.”
t0“alfalfa”
Claim · model-written summary
Hypocotyl was the best explant for rapid callus induction.
- Taxon
- alfalfaMedicago sativat0
- Organ / stage
- Hypocotyle0
- Treatment
- Hypocotyle0
- Outcome
- rapid callus inductione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hypocotyl was the best explant for rapid callus induction.”
t0“alfalfa”
Claim · model-written summary
Longdong hypocotyl callus induction approached 100% on MSH.
- Taxon
- alfalfaMedicago sativat0
- Material
- Longdonge0
- Organ / stage
- hypocotyle0
- Treatment
- MSHe0
- Outcome
- callus induction ratee0
- Value
- close to 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hypocotyl callus induction rate of Longdong and Daye was close to 100% on MSH.”
t0“alfalfa”
Claim · model-written summary
Daye hypocotyl callus induction approached 100% on MSH.
- Taxon
- alfalfaMedicago sativat0
- Material
- Dayee0
- Organ / stage
- hypocotyle0
- Treatment
- MSHe0
- Outcome
- callus induction ratee0
- Value
- close to 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hypocotyl callus induction rate of Longdong and Daye was close to 100% on MSH.”
t0“alfalfa”
Claim · model-written summary
SH medium was most efficient for inducing somatic embryos.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- SH mediume0
- Outcome
- inducing somatic embryose0
- Direction
- most efficiente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SH medium was the most efficient for inducing somatic embryos”
t0“alfalfa”
Claim · model-written summary
Somatic embryo induction on SH medium reached 72% in Longdong.
- Taxon
- alfalfaMedicago sativat0
- Material
- Longdonge0
- Outcome
- somatic embryos induction ratee0
- Value
- 72%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the somatic embryos induction rate of Longdong and Daye was up to 72% and 58% respectively.”
t0“alfalfa”
Claim · model-written summary
Somatic embryo induction on SH medium reached 58% in Daye.
- Taxon
- alfalfaMedicago sativat0
- Material
- Dayee0
- Outcome
- somatic embryos induction ratee0
- Value
- 58%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the somatic embryos induction rate of Longdong and Daye was up to 72% and 58% respectively.”
t0“alfalfa”
Claim · model-written summary
Half-strength MS with 10 g/L sucrose favored rooting and seedling of somatic embryos.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- 1/2MS with 10 g/L sucrosee0
- Outcome
- rooting and seedling of somatic embryose0
- Direction
- propitiouse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1/2MS with 10 g/L sucrose was propitious for rooting and seedling of somatic embryos.”
t0“alfalfa”
Claim · model-written summary
Proline had no effect on somatic embryo induction and differentiation.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- Prolinee0
- Outcome
- induction and differentiation of somatic embryose0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proline had no effect on induction and differentiation of somatic embryos”
t0“alfalfa”
Claim · model-written summary
Adding active carbon to SH medium reduced somatic embryo induction.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- adding active carbon in SH mediume0
- Outcome
- somatic embryos induction ratese0
- Direction
- reducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“adding active carbon in SH medium can reduce somatic embryos induction rates”
t0“alfalfa”
Claim · model-written summary
Somatic embryo induction was highest at 20 g/L sucrose.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- sucrosee0
- Outcome
- somatic embryos induction ratee0
- Direction
- higheste0
- Value
- 20 g/Le0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest somatic embryos induction rate for sucrose was 20 g/L.”
t0“alfalfa”
Claim · model-written summary
The established regeneration system was judged beneficial to AtNHX1 transformation of alfalfa.
- Taxon
- alfalfaMedicago sativae0
- Gene
- AtNHX1e0
- Outcome
- AtNHX1 gene transformation of alfalfae0
- Value
- high frequencye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The establishment of high frequency regeneration system of alfalfa somatic embryos benefits the AtNHX1 gene transformation of alfalfa greatly.”
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See all 28 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.