Abstract
The leaves of the two varieties of Medicago sativa L.cv.Xinjiang Daye and Medicago varia Martyn.cv.Xinmu No.1 were cultured in four kinds of induction medium to study the induction of callus,effects of CH on enrichment,and differentiation callus in differentiation media with KT.The results were as follows: ①MS+2,4-D 2.00 mg/L+NAA 1.00 mg/L+KT 1.00 mg/L was the better induction medium,the callus ratio of Medicago varia Martyn.cv.Xinmu No.1 and Medicago sativa L.cv.Xinjiang Daye separately were 100%,95.5% respectively;②Adding CH 0.15 g/L in enrichment medium was benefit to the growth of Medicago varia Martyn.cv.Xinmu No.1,CH 1.00 g/L was propitious to the growth of Medicago sativa L.cv.Xinjiang Daye;③With KT 4.00 mg/L in differentiation medium,the differentiation rate of Medicago varia Martyn.cv.Xinmu No.1 was 25.0% and Medicago sativa L.cv.Xinjiang Daye was 13.2%.
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Claims 6
Claim · model-written summary
MS+2,4-D+NAA+KT medium was the better callus induction medium for Medicago varia Xinmu No.1, with a callus ratio of 100%.
Verbatim
e0“The leaves of the two varieties of Medicago sativa L.cv.Xinjiang Daye and Medicago varia Martyn.cv.Xinmu No.1 were cultured in four kinds of induction medium”
e1“MS+2,4-D 2.00 mg/L+NAA 1.00 mg/L+KT 1.00 mg/L was the better induction medium,the callus ratio of Medicago varia Martyn.cv.Xinmu No.1 and Medicago sativa L.cv.Xinjiang Daye separately were 100%,95.5% respectively”
Claim · model-written summary
MS+2,4-D+NAA+KT medium was the better callus induction medium for Medicago sativa Xinjiang Daye, with a callus ratio of 95.5%.
- Taxon
- Medicago sativa L.cv.Xinjiang Daye
Medicago sativae1 - Material
- Xinjiang Dayee1
- Organ / stage
- leavese0
- Treatment
- MS+2,4-D 2.00 mg/L+NAA 1.00 mg/L+KT 1.00 mg/Le1
- Outcome
- callus ratioe1
- Direction
- bettere1
- Value
- 95.5%e1
- Comparator
- four kinds of induction mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The leaves of the two varieties of Medicago sativa L.cv.Xinjiang Daye and Medicago varia Martyn.cv.Xinmu No.1 were cultured in four kinds of induction medium”
e1“MS+2,4-D 2.00 mg/L+NAA 1.00 mg/L+KT 1.00 mg/L was the better induction medium,the callus ratio of Medicago varia Martyn.cv.Xinmu No.1 and Medicago sativa L.cv.Xinjiang Daye separately were 100%,95.5% respectively”
Claim · model-written summary
CH at 0.15 g/L in enrichment medium benefited growth of Medicago varia Xinmu No.1 callus.
- Taxon
- Medicago varia Martyn.cv.Xinmu No.1e2
- Material
- Xinmu No.1e2
- Treatment
- CH 0.15 g/Le2
- Outcome
- growthe2
- Direction
- benefite2
Not reportedComparator
Verbatim
e2“Adding CH 0.15 g/L in enrichment medium was benefit to the growth of Medicago varia Martyn.cv.Xinmu No.1”
Claim · model-written summary
CH at 1.00 g/L was propitious to growth of Medicago sativa Xinjiang Daye callus.
- Taxon
- Medicago sativa L.cv.Xinjiang DayeMedicago sativae3
- Material
- Xinjiang Dayee3
- Treatment
- CH 1.00 g/Le3
- Outcome
- growthe3
- Direction
- propitiouse3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“CH 1.00 g/L was propitious to the growth of Medicago sativa L.cv.Xinjiang Daye”
Claim · model-written summary
Differentiation rate of Medicago varia Xinmu No.1 was 25.0% on differentiation medium with KT 4.00 mg/L.
Verbatim
e4“With KT 4.00 mg/L in differentiation medium,the differentiation rate of Medicago varia Martyn.cv.Xinmu No.1 was 25.0% and Medicago sativa L.cv.Xinjiang Daye was 13.2%”
Claim · model-written summary
Differentiation rate of Medicago sativa Xinjiang Daye was 13.2% on differentiation medium with KT 4.00 mg/L.
- Taxon
- Medicago sativa L.cv.Xinjiang DayeMedicago sativae4
- Material
- Xinjiang Dayee4
- Treatment
- KT 4.00 mg/Le4
- Outcome
- differentiation ratee4
- Value
- 13.2%e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“With KT 4.00 mg/L in differentiation medium,the differentiation rate of Medicago varia Martyn.cv.Xinmu No.1 was 25.0% and Medicago sativa L.cv.Xinjiang Daye was 13.2%”
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