Abstract
The aim of the study was to evaluate alfalfa material on drought tolerance to distinguish the genotypes, which are resistant to water deficit at early stages of growth. The study was carried out during 2016-2018 in laboratory conditions at the Institute of Irrigated Agriculture of NAAS (Ukraine) using alfalfa seeds of different genotypes were subjected to germination in sucrose osmotic solutions with a pressure of 0.3, 0.5, 0.7 MPa within 7 days in the thermostat at the temperature 20 ± 2ºC. The control variant - purified water. The highest germination rate of alfalfa seeds was observed in the purified water - 82.0-96.1%. The increase of osmotic pressure in sucrose solutions decreased germination rate of seeds. Root length and hypocotyl length reached the highest values in the purified water – 24.1-33.9 and 26.1-34.1 mm, respectively. Sucrose osmotic solution with the pressure of 3.04 bar oppressed the growth of root length and hypocotyl length, which at this variant reached 11.4-19.9 and 13.8-21.9 mm, respectively. Besides, different alfalfa genotypes showed different reaction to the osmotic stress. The highest values of vegetation growth intensity (VI) in the osmotic solution with the pressure of 0.3 MPa were recorded for genotypes MgP and Unitro - 31.7 and 34.1, respectively. The values of the index at 0.5 MPa decreased in the genotypes to 19.4 and 17.6, respectively. Particular attention should be paid to the genotypes Kazachka No.2 and Pr, which are characterized with a gradual decrease in VI with the increase of osmotic pressure from 0.3 MPa to 0.7 MPa in the solution: 20.3, 13.1, 10.5 and 25.1, 19.9, 7.3, respectively. The genotypes Kazachka No.2, Pr, Unitro had the best indices of germination rate, root, and hypocotyl length, VI values, and are recommended as the most prospective in alfalfa breeding for drought tolerance.
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Claims 14
Claim · model-written summary
Alfalfa seed germination was highest in purified water at 82.0-96.1%.
- Taxon
- alfalfa
Medicago sativae0 - Treatment
- purified watere0
- Outcome
- germination ratee0
- Direction
- higheste0
- Value
- 82.0-96.1%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest germination rate of alfalfa seeds was observed in the purified water - 82.0-96.1%.”
Claim · model-written summary
Increasing osmotic pressure in sucrose solutions decreased alfalfa seed germination.
- Taxon
- ALFALFAMedicago sativat0
- Treatment
- increase of osmotic pressure in sucrose solutionse0
- Outcome
- germination rate of seedse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase of osmotic pressure in sucrose solutions decreased germination rate of seeds.”
t0“ALFALFA (Medicago sativa)”
Claim · model-written summary
Alfalfa root length was highest in purified water at 24.1-33.9 mm.
- Taxon
- ALFALFAMedicago sativat0
- Treatment
- purified watere0
- Outcome
- Root lengthe0
- Direction
- higheste0
- Value
- 24.1-33.9e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root length and hypocotyl length reached the highest values in the purified water – 24.1-33.9 and 26.1-34.1 mm, respectively.”
t0“ALFALFA (Medicago sativa)”
Claim · model-written summary
Alfalfa hypocotyl length was highest in purified water at 26.1-34.1 mm.
- Taxon
- ALFALFAMedicago sativat0
- Treatment
- purified watere0
- Outcome
- hypocotyl lengthe0
- Direction
- higheste0
- Value
- 26.1-34.1 mme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root length and hypocotyl length reached the highest values in the purified water – 24.1-33.9 and 26.1-34.1 mm, respectively.”
t0“ALFALFA (Medicago sativa)”
Claim · model-written summary
At 3.04 bar sucrose osmotic pressure, root length was suppressed to 11.4-19.9 mm.
- Taxon
- ALFALFAMedicago sativat0
- Treatment
- Sucrose osmotic solution with the pressure of 3.04 bare0
- Outcome
- root lengthe0
- Direction
- oppressede0
- Value
- 11.4-19.9e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose osmotic solution with the pressure of 3.04 bar oppressed the growth of root length and hypocotyl length, which at this variant reached 11.4-19.9 and 13.8-21.9 mm, respectively.”
t0“ALFALFA (Medicago sativa)”
Claim · model-written summary
At 3.04 bar sucrose osmotic pressure, hypocotyl length was suppressed to 13.8-21.9 mm.
- Taxon
- ALFALFAMedicago sativat0
- Treatment
- Sucrose osmotic solution with the pressure of 3.04 bare0
- Outcome
- hypocotyl lengthe0
- Direction
- oppressede0
- Value
- 13.8-21.9 mme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose osmotic solution with the pressure of 3.04 bar oppressed the growth of root length and hypocotyl length, which at this variant reached 11.4-19.9 and 13.8-21.9 mm, respectively.”
t0“ALFALFA (Medicago sativa)”
Claim · model-written summary
Alfalfa genotypes differed in response to osmotic stress.
- Taxon
- alfalfaMedicago sativae0
- Outcome
- reaction to the osmotic stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Besides, different alfalfa genotypes showed different reaction to the osmotic stress.”
Claim · model-written summary
MgP had a vegetation growth intensity of 31.7 at 0.3 MPa.
- Taxon
- ALFALFAMedicago sativat0
- Material
- MgPe0
- Treatment
- osmotic solution with the pressure of 0.3 MPae0
- Outcome
- vegetation growth intensitye0
- Value
- 31.7e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest values of vegetation growth intensity (VI) in the osmotic solution with the pressure of 0.3 MPa were recorded for genotypes MgP and Unitro - 31.7 and 34.1, respectively.”
t0“ALFALFA (Medicago sativa)”
Claim · model-written summary
Unitro had a vegetation growth intensity of 34.1 at 0.3 MPa.
- Taxon
- ALFALFAMedicago sativat0
- Material
- Unitroe0
- Treatment
- osmotic solution with the pressure of 0.3 MPae0
- Outcome
- vegetation growth intensitye0
- Value
- 34.1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest values of vegetation growth intensity (VI) in the osmotic solution with the pressure of 0.3 MPa were recorded for genotypes MgP and Unitro - 31.7 and 34.1, respectively.”
t0“ALFALFA (Medicago sativa)”
Claim · model-written summary
MgP vegetation growth intensity decreased to 19.4 at 0.5 MPa.
- Taxon
- ALFALFAMedicago sativat0
- Material
- MgPe1
- Treatment
- 0.5 MPae0
- Outcome
- indexe0
- Direction
- decreasede0
- Value
- 19.4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The values of the index at 0.5 MPa decreased in the genotypes to 19.4 and 17.6, respectively.”
e1“genotypes MgP and Unitro - 31.7 and 34.1, respectively.”
t0“ALFALFA (Medicago sativa)”
Claim · model-written summary
Unitro vegetation growth intensity decreased to 17.6 at 0.5 MPa.
- Taxon
- ALFALFAMedicago sativat0
- Material
- Unitroe1
- Treatment
- 0.5 MPae0
- Outcome
- indexe0
- Direction
- decreasede0
- Value
- 17.6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The values of the index at 0.5 MPa decreased in the genotypes to 19.4 and 17.6, respectively.”
e1“genotypes MgP and Unitro - 31.7 and 34.1, respectively.”
t0“ALFALFA (Medicago sativa)”
Claim · model-written summary
Kazachka No.2 vegetation growth intensity fell gradually across 0.3 to 0.7 MPa, with values 20.3, 13.1 and 10.5.
- Taxon
- alfalfaMedicago sativae0
- Material
- Kazachka No.2e1
- Treatment
- increase of osmotic pressure from 0.3 MPa to 0.7 MPae1
- Outcome
- VIe1
- Direction
- gradual decreasee1
- Value
- 20.3, 13.1, 10.5e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genotypes Kazachka No.2, Pr, Unitro had the best indices of germination rate, root, and hypocotyl length, VI values, and are recommended as the most prospective in alfalfa breeding for drought tolerance.”
e1“Particular attention should be paid to the genotypes Kazachka No.2 and Pr, which are characterized with a gradual decrease in VI with the increase of osmotic pressure from 0.3 MPa to 0.7 MPa in the solution: 20.3, 13.1, 10.5 and 25.1, 19.9, 7.3, respectively.”
Claim · model-written summary
Pr vegetation growth intensity fell across 0.3 to 0.7 MPa, with values 25.1, 19.9 and 7.3.
- Taxon
- alfalfaMedicago sativae0
- Material
- Pre1
- Treatment
- increase of osmotic pressure from 0.3 MPa to 0.7 MPae1
- Outcome
- VIe1
- Direction
- gradual decreasee1
- Value
- 25.1, 19.9, 7.3e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genotypes Kazachka No.2, Pr, Unitro had the best indices of germination rate, root, and hypocotyl length, VI values, and are recommended as the most prospective in alfalfa breeding for drought tolerance.”
e1“Particular attention should be paid to the genotypes Kazachka No.2 and Pr, which are characterized with a gradual decrease in VI with the increase of osmotic pressure from 0.3 MPa to 0.7 MPa in the solution: 20.3, 13.1, 10.5 and 25.1, 19.9, 7.3, respectively.”
Claim · model-written summary
Kazachka No.2, Pr and Unitro were recommended as promising breeding material for drought tolerance.
- Taxon
- alfalfaMedicago sativae0
- Outcome
- alfalfa breeding for drought tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genotypes Kazachka No.2, Pr, Unitro had the best indices of germination rate, root, and hypocotyl length, VI values, and are recommended as the most prospective in alfalfa breeding for drought tolerance.”
The same questions in other papers
sucrose · growth · Medicago sativa (alfalfa)Positive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
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- positive
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